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Japanese pondcrayfish reovirus gene diagnosis reagent kit

A technology for reovirus and gene diagnosis, which is applied in the field of gene diagnosis kits for Macrobrachium japonica reovirus, and can solve the problem of lack of virus detection technology and the like

Inactive Publication Date: 2009-08-19
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are currently no patents or reports on this virus detection technology

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  • Japanese pondcrayfish reovirus gene diagnosis reagent kit
  • Japanese pondcrayfish reovirus gene diagnosis reagent kit
  • Japanese pondcrayfish reovirus gene diagnosis reagent kit

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Embodiment Construction

[0022] 1. The composition of several reagents in this kit

[0023] 1. Reagent A solution and reagent B solution are commonly used reagents.

[0024] 2. Reagent C solution

[0025] Reagent C solution is a reverse transcription reaction solution containing Macrobrachium reovirus RNA, and the composition of each tube is:

[0026] 4 μl 5× RNA reverse transcription buffer,

[0027] 2 μl 10 mM MgCl 2 ,

[0028] 8 μl RNase Free H 2 O,

[0029] 1μl 10mM dNTP Mixture,

[0030] 1 μl 1u RNsin,

[0031] 1 μl 1u M-MLV Reverse Transcriptase,

[0032] 1 μl of 10 pM MnRV-325R.

[0033] 3. Reagent D solution

[0034] Reagent D solution is a PCR reaction solution, and the composition of each tube is:

[0035] 2mM MgCl 2 ,

[0036] 5 μl 10× PCR buffer,

[0037] 41 μl ddH 2 O,

[0038] 2U Taq enzyme,

[0039] 10 pM MnRV-325R and MnRV-325F.

[0040] 2. The method of using this kit is as follows:

[0041] ① Homogenate of Macrobrachium japonicus tissue

[0042] Add 100 μl reagent...

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Abstract

The invention discloses a macrobrachium nipponensis reovirus gene diagnosis kit, and relates to a virogene diagnosis kit. The kit comprises the following reagents: reagent A is tissue homogenate buffer solution which is Tris-NaCl-EDTA buffer solution; reagent B is RNA extracting solution which is Trizol liquid; reagent C is macrobrachium nipponensis reovirus RNA reverse transcription reaction liquid which contains special primer MnRV-325R; reagent D is PCR reaction liquid that contains special primer MnRV 325R and MnRV 325F; reagent E liquid is positive control which is an MnRV positive DNA template. The kit can be used for rapidly, sensitively and exactly testing whether young macrobrachium nipponensis, shrimp, grown shrimp and parent shrimp carry the macrobrachium nipponensis reovirus or not.

Description

technical field [0001] The invention relates to a virus gene diagnosis kit, in particular to a macrobrachium reovirus gene diagnosis kit. Background technique [0002] River prawn (Macrobrachium nipponensede Haan), also known as freshwater prawn, scientifically known as giant river prawn, is a unique freshwater prawn in Japan and my country. It is rich in nutrition, tender and delicious, and is a popular and precious aquatic product. In the process of studying river shrimp diseases, a strain of reovirus (named MnRV, M acrobrachium n ipponensede R eo v irus). There is currently no cure for the viral disease. Therefore, the establishment of sensitive and accurate early detection technology is of great significance for disease prevention and economic loss reduction. There are no patents and reports about this virus detection technology at present. Contents of the invention [0003] The purpose of the present invention is to fill up the gap in the prior art and provide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 张叔勇周丽荣
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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