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Method for preparing matrine/carboxymethyl chitosan/phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation

A technology of phosphorylated chitosan and carboxymethyl chitosan, applied in the fields of botanical equipment and methods, agriculture, biocide, etc., can solve the problems of easy decomposition of active ingredients, complex preparation ingredients, unstable to light, etc. To achieve the effect of easy preparation, favorable adhesion and penetration, and avoiding the use of organic solvents and other additives

Active Publication Date: 2012-06-06
南京善思生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with synthetic pesticides, there are still disadvantages of botanical pesticides: slow efficacy, easy decomposition of active ingredients, instability to light, and short duration
In addition, except for a few water-soluble plant-derived pesticides, most of them require artificial synthetic auxiliaries, and the composition of the preparation is complex, which is not conducive to environmental protection.

Method used

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  • Method for preparing matrine/carboxymethyl chitosan/phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation
  • Method for preparing matrine/carboxymethyl chitosan/phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation
  • Method for preparing matrine/carboxymethyl chitosan/phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of phosphorylated chitosan: The viscosity average molecular weight of 5g is 8.5×10 4 , The chitosan with a degree of deacetylation of 95% is dissolved in 250ml of 1% acetic acid solution by volume, and the temperature is raised to 60°C. Phosphorous acid solution with a mass volume concentration of 0.25g / ml is added; 15ml of mass percentage is added 37% formaldehyde solution, stirred at 70℃ for 1h; pour the reaction solution into absolute ethanol to precipitate, filter, collect the precipitate, soak and wash twice with absolute ethanol, freeze-dry at -20℃ for 24h to obtain phosphorylation Chitosan

[0024] (2) Preparation of matrine / carboxymethyl chitosan / phosphorylated chitosan nanoparticle water dispersion: 5ml of matrine with a concentration of 1.5mg / ml (the chemical structure of matrine is as figure 1 Shown) The aqueous solution is mixed with 5 ml of the carboxymethyl chitosan aqueous solution with a concentration of 0.35 mg / ml, and the viscosity average ...

Embodiment 2

[0026] (1) Preparation of phosphorylated chitosan: 10g viscosity average molecular weight is 8.5×10 4 , Chitosan with a degree of deacetylation of 95% is dissolved in 500ml of acetic acid solution with a volume percentage of 1.5%, the temperature is raised to 75℃, and a phosphorous acid solution with a mass volume concentration of 0.5g / ml is added; 6ml is added with a mass percentage of 40% formaldehyde solution, stirred and reacted at 80℃ for 5.5h; Pour the reaction solution into absolute ethanol to precipitate, filter, collect the precipitate, soak and wash 3 times with absolute ethanol, freeze-dry at -30℃ for 48h to obtain phosphoric acid Chitosan;

[0027] (2) Preparation of matrine / carboxymethyl chitosan / phosphorylated chitosan nanoparticle water dispersion: 10ml of matrine aqueous solution with a concentration of 1.0mg / ml and 15ml of carboxymethyl with a concentration of 0.5mg / ml Mix the methyl chitosan aqueous solution, the viscosity average molecular weight of the carboxy...

Embodiment 3

[0029] (1) Preparation of phosphorylated chitosan: 8g viscosity average molecular weight is 2.2×10 5 , Chitosan with a degree of deacetylation of 92% is dissolved in 350ml of 1.2% acetic acid solution, heated to 65°C, added with a phosphorous acid solution with a mass volume concentration of 0.45g / ml; added 10ml with a mass percentage of 38% formaldehyde solution, stirred for 2h at 75℃; pour the reaction solution into absolute ethanol to precipitate, filter, collect the precipitate, soak and wash twice with absolute ethanol, freeze-dry at -10℃ for 38h to obtain phosphorylation Chitosan

[0030] (2) Preparation of matrine / carboxymethyl chitosan / phosphorylated chitosan nanoparticle water dispersion: 8ml of matrine aqueous solution with a concentration of 2.0mg / ml and 10ml of carboxymethyl with a concentration of 0.25mg / ml Mix the methyl chitosan aqueous solution, the viscosity average molecular weight of the carboxymethyl chitosan is 5.3×10 5 Under stirring conditions, add the abov...

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Abstract

The invention discloses a preparation method and use of a matrine / carboxymethyl chitosan / phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation. The method comprises the following The invention discloses a preparation method and use of a matrine / carboxymethyl chitosan / phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation. The method comprises the followingsteps: (1) preparing phosphonic chitosan; and (2) preparing matrine / carboxymethyl chitosan / phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation. The method overcomes the water insteps: (1) preparing phosphonic chitosan; and (2) preparing matrine / carboxymethyl chitosan / phosphonic chitosan pesticide nanoparticle aqueous dispersion preparation. The method overcomes the water insoluble drawback of chitosan and provides a reference of the new agricultural use of the chitosan and derivatives thereof; An pesticide nanoparticle aqueous dispersion preparation is advantageous oversoluble drawback of chitosan and provides a reference of the new agricultural use of the chitosan and derivatives thereof; An pesticide nanoparticle aqueous dispersion preparation is advantageous overa simple water aqueous solution preparation in insect killing effect; after a botanical pesticide is nano-sized, the nano particles can protect and stabilize the pesticide to allow the pesticide to r a simple water aqueous solution preparation in insect killing effect; after a botanical pesticide is nano-sized, the nano particles can protect and stabilize the pesticide to allow the pesticide to release slowly in a controlled way; the preparation method is easy to implement and avoids use of organic solvent and assistant; and degradation products of the chitosan and the derivatives of the chitelease slowly in a controlled way; the preparation method is easy to implement and avoids use of organic solvent and assistant; and degradation products of the chitosan and the derivatives of the chitosan can adjust the growth of vegetables, and nitrogen and phosphor in the degradation products are fertilizers of vegetables, so the preparation of the invention is a real environmentally-friendly peosan can adjust the growth of vegetables, and nitrogen and phosphor in the degradation products are fertilizers of vegetables, so the preparation of the invention is a real environmentally-friendly pesticide.sticide.

Description

Technical field [0001] The invention belongs to the field of natural polymer modification and agricultural pesticide formulations, and particularly relates to a preparation method and application of a matrine / carboxymethyl chitosan / phosphorylated chitosan pesticide nanoparticle water dispersion formulation. Background technique [0002] Chitosan, a natural polysaccharide with cations, is favored by people because of its unique biological properties. However, chitosan can only dissolve in acidic solutions, which limits its direct application. On the one hand, as a drug carrier, acidic media can easily cause toxicity to organisms and interference with drug performance; on the other hand, chitosan molecules will precipitate out in aqueous solutions with neutral pH. This performance limits its application as a pesticide and drug carrier. Therefore, seeking a water-soluble approach to chitosan and preparing its water-soluble derivatives is the key to applying chitosan as a matrix ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N57/24A01N43/90A01N43/16A01N25/04A01N25/22A01P7/04A01P21/00C05G3/02C05G3/60
Inventor 张子勇殷旭东
Owner 南京善思生态科技有限公司