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Test strip for fast testing nitrate in food via enzyme method

A rapid determination and nitrate technology, applied in the field of detection, can solve the problems of few detection reports, and achieve the effect of strong specificity and convenient use.

Inactive Publication Date: 2009-09-02
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the use of biochemical methods to reduce nitrate to nitrite and apply it to the detection of nitrate content

Method used

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  • Test strip for fast testing nitrate in food via enzyme method
  • Test strip for fast testing nitrate in food via enzyme method
  • Test strip for fast testing nitrate in food via enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of embodiment 1 test strip of the present invention

[0019] Preparation of reduction zone: cut the quantitative filter paper into 0.6cm×10.0cm strips, soak in 7000.0μmol·L -1 Nitrate reductase, 10.0mmol·L -1 0.1mol L of NAD(P)H -1 After phosphate buffer (pH7.5) for 15 minutes, dry at 35°C or blow dry with cold air, and cut the test paper into test paper with a size of 0.6cm×0.8cm;

[0020] Preparation of color zone: cut quantitative filter paper into 0.6cm×10.0cm strips, soak in 4.5% N-1-naphthylethylenediamine dihydrochloric acid distilled aqueous solution for 15min, dry at 35°C or blow with cold wind Dry; then soak in an equal-volume mixed distilled aqueous solution of 7.5% p-aminobenzenesulfonic acid and 15.0% tartaric acid for 15 minutes, then dry at 35°C or blow dry with cold wind; cut the test paper into 0.6cm×0.8cm test strips;

[0021] Preparation of test paper strips: stick a color-developing zone test paper piece to one end of the p...

Embodiment 2

[0022] Embodiment 2 Reagent strip of the present invention quickly measures food method

[0023] Take a few drops of food juice (2 to 3 drops is appropriate) and drop it on the reduction area. After 1 to 2 minutes, set up a test strip, let the reduced juice flow down to the lower color development area for color reaction, and after 1 to 2 minutes, compare with the standard Color scales were compared to estimate nitrate ranges. Table 1 is the comparison between the enzymatic nitrate test strip and the national standard method (GB / T5009.33-2003) to detect the nitrate content in several fresh vegetables. Although the nitrate content in fresh vegetables detected by the enzymatic nitrate test strip method was lower than the corresponding content by the national standard method, the differences were not significant (p>0.05). The results show that the nitrate test strip method can basically meet the detection accuracy; it also shows good sensitivity to peppers and cucumbers with low...

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Abstract

The invention provides a test strip for fast testing nitrate in food via the biochemical reduction method of nitrate reductase. System of the nitrate reductase + reduced coenzyme I (namely, nicotinamide adenine dinucleotide NADP) or system of the nitrate reductase + reduced coenzyme II (namely, nicotinamide adenine dinucleotide phosphate NADPH) catalyzes the nitrate to generate nitrite via the way of reduction. The test trip is applied to fast test nitrate content in food. The test trip is convenient in use, strong in specificity and beneficial to fast testing nitrate content in food.

Description

technical field [0001] The invention belongs to the technical field of detection, and relates to a technology for detecting nitrate content, in particular to a test strip for quickly measuring nitrate in food by utilizing nitrate reductase to reduce nitrate to generate nitrite and an application thereof. Background technique [0002] At present, the significance of detecting nitrate content in food (including edible agricultural products, the same below) is very important. Nitrate itself is not harmful, but after being ingested by the human body, it can be converted into nitrite under the action of nitrate-reducing bacteria. Nitrite can make hemoglobin lose its oxygen-carrying capacity and cause poisoning; in addition, nitrite can form evidence under certain circumstances. Carcinogenic nitrosamine compounds. Now food safety and quality issues are getting more and more attention in the domestic market and export trade. Therefore, it is of great significance to study the met...

Claims

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Application Information

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IPC IPC(8): C12P3/00G01N21/78
Inventor 孙京新周光宏王世清赵向进高科罗欣张欣
Owner QINGDAO AGRI UNIV
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