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Method for preparing monosaccharide by using raw material containing cellulose

A technology for cellulose and raw materials, applied in the field of preparing monosaccharides, can solve the problems of reducing the proportion of cellulose, reducing the yield of monosaccharides, low enzymatic hydrolysis efficiency, etc., to improve the yield, improve the sugar conversion rate, and improve the enzymatic hydrolysis efficiency. Effect

Active Publication Date: 2012-01-04
COFCO GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to overcome the disadvantages of low monosaccharide yield and low enzymolysis efficiency of the existing method for preparing monosaccharide from cellulose-containing raw materials, and provide a method with high monosaccharide yield and high enzymolysis efficiency. Method for preparing monosaccharides from cellulose-containing raw materials
The presumed reason may be that due to the large amount of cellulose-containing raw materials put into the steam explosion device in each batch, and the difference in the accumulation mode of each batch of raw materials or the instability of steam explosion conditions, it is impossible to ensure that all the cellulose-containing raw materials The cellulose raw material fully absorbs the water vapor within a few minutes after being steamed, therefore, there will still be most of the cellulose-containing raw materials that cannot be completely exploded by the water vapor inside because they cannot fully absorb the water vapor. As a result, the lignin sheath embedding cellulose and hemicellulose in cellulose-containing raw materials cannot be fully destroyed, reducing the proportion of cellulose in cellulose-containing raw materials that can be contacted with enzymes, thereby reducing the yield of monosaccharides

Method used

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  • Method for preparing monosaccharide by using raw material containing cellulose
  • Method for preparing monosaccharide by using raw material containing cellulose
  • Method for preparing monosaccharide by using raw material containing cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This example illustrates the method of the present invention for the preparation of monosaccharides from cellulose-containing feedstocks.

[0062] (1) Continuous steam explosion

[0063] test as figure 1 For the corn stalk raw material shown, the water content of the corn stalk measured by the above-mentioned method is 20% by weight, the raw material is cut into small sections of 1.5 cm × 0.2 cm × 0.5 cm, and the corn stalk section with a water content of 20% by weight is It is fed continuously from the feeder, and water drenched before being transported to the heat preservation pressure device, and the water drench makes the water content of the corn stalks 40% by weight. Then 2000 grams of corn stalks with a water content of 40% by weight are continuously transported to the above-mentioned heat preservation pressure device, the pressure of the heat preservation pressure device is 1.6 MPa, and the temperature is 195 ° C. Continuous transmission, the linear speed of t...

Embodiment 2

[0071] This example illustrates the method of the present invention for preparing monosaccharides from cellulose-containing feedstocks.

[0072] Monosaccharides were prepared according to the method of Example 1, except that the corn stalks with a water content of 20% by weight were water drenched to make the water content of the corn stalks 75% by weight. Then according to the conditions and methods of Example 1, 4800 grams of corn stalks with a water content of 75% by weight are continuously delivered to the heat preservation pressure device for continuous steam explosion, and the steam explosion waste is collected and washed to obtain a total of 6000 grams of solid steam explosion waste ( The water content is 80% by weight). According to the method of HPLC analysis of the steam explosion product of sulfuric acid hydrolysis described in Example 1, record 6000 grams of steam explosion product containing 520 grams of cellulose and 144.4 grams of hemicellulose.

[0073] Accord...

Embodiment 3

[0075] This example illustrates the method of the present invention for preparing monosaccharides from cellulose-containing feedstocks.

[0076] Monosaccharides were prepared according to the method of Example 1, the difference was that 1500 grams of corn stalks with a water content of 20% by weight were continuously fed from the feeder and directly transported to the heat preservation pressure device for continuous Steam explosion, the condition of steam explosion is the same as embodiment 1, obtains 2400 grams of solid steam explosion waste (water content is 50% by weight) altogether after collecting steam explosion waste and washing.

[0077] According to the method of HPLC analysis of the steam explosion product of sulfuric acid hydrolysis described in Example 1, record 2400 grams of steam explosion product containing 515.2 grams of cellulose and 142.2 grams of hemicellulose.

[0078] According to the method of Example 1, the above-mentioned steam explosion product was enz...

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Abstract

The invention discloses a method for preparing monosaccharide by using a raw material containing cellulose, which comprises the steps of subjecting the raw material containing cellulose to steam explosion and mixing an obtained product of the steam explosion with an enzyme for enzymolysis, wherein a method for the steam explosion of the raw material containing cellulose comprises the steps of continuously delivering the raw material containing cellulose to a heat-preservation pressure unit, keeping the raw material containing cellulose in the heat-preservation pressure unit for 3 to 10 minutes first and then releasing part of the raw material containing cellulose in the heat-preservation pressure unit to normal pressure every other 5 to 12 seconds; and a method for mixing the product of the steam explosion and the enzyme comprises the step of mixing part of the product of steam explosion with the enzyme first to obtain a mixture and then continuously adding the rest production of the steam explosion into the mixture for mixing, wherein the rest product of the steam explosion is added at a speed that keeps the concentration of the product of the steam explosion in the reaction system no more than 150g / L and the weight of the part, mixed with the enzyme first, of the product of the steam explosion accounts for 10 to 30 percent of the total weight of the product of the steam explosion. The method can improve the monosaccharide yield effectively.

Description

technical field [0001] The present invention relates to a method for the preparation of sugars, and more particularly to a method for the preparation of monosaccharides from cellulose-containing raw materials. Background technique [0002] The existing technology can use cellulose-containing raw materials to prepare monosaccharides, and the obtained monosaccharides are mainly glucose. Glucose can be used in the food and pharmaceutical industries, and can be used as a reducing agent in the printing and dyeing leather industry. Glucose is often used as a reducing agent in the silver process; the obtained monosaccharides also include xylose, which can be hydrogenated to produce xylitol as a non-caloric sweetener for food. The monosaccharides can also be fermented by microorganisms (such as yeast) to produce ethanol. The cellulose-containing raw materials are generally roots, stems and leaves of plants, including straws. The straw refers to the remaining roots, stems and leave...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02
Inventor 袁敬伟岳国君李春玲孙长征解成华林海龙
Owner COFCO GROUP
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