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Preparation method of microecological preparation of lactobacillus

A technology of micro-ecological preparations and lactobacilli, applied in the direction of bacteria, animal feed, additional food elements, etc., can solve the problems of poor quality and high production costs, and achieve the effects of stable product quality, reduced production costs, and easy operation

Active Publication Date: 2009-09-16
福建大北农华有水产科技集团有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of poor quality and high production cost in current Lactobacillus microecological preparations, and to meet the comprehensive promotion and use of microecological feed additives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 Preparation of a kind of insoluble lactobacillus probiotics

[0026] (1) Expanded culture of Lactobacillus

[0027] ①First-level culture: use conventional MRS medium as the slant medium to prepare the first-level seed slant, and cultivate at 37°C for 26 hours;

[0028] ②Secondary liquid culture, using fermentation medium, 37°C, ph4.5-6.0, shaking culture at 150rpm for 18 hours;

[0029] The formula of the fermentation medium is: glucose 3.2%, yeast extract 1.7%, soybean peptone 2.1%, ammonium sulfate 2.7%, calcium carbonate 3.1%;

[0030] ③ Three-stage fermentation culture, adopt the fermentation medium used in the two-stage cultivation, and cultivate in a fermenter, the inoculum size is 1%, the liquid volume is 60%, the rotating speed is 80rpm, the air ratio is 1:0.2, the temperature is 37°C, and the pH is 4.5 —5.0, cultivated for 22 hours to obtain a fermented liquid;

[0031] At this time, the number of live bacteria in the fermentation broth was 4.5×...

Embodiment 2

[0041] Embodiment 2 Preparation of a kind of soluble lactobacillus probiotics

[0042] (1) Expanded culture of Lactobacillus

[0043] ①First-level culture: use conventional MRS medium as the slant medium to prepare the first-level seed slant, and cultivate at 37°C for 30 hours;

[0044] ②Secondary liquid culture, using fermentation medium, 37°C, ph4.5-6.0, shaking culture at 170rpm for 18 hours;

[0045] The formula of the fermentation medium is: glucose 5%, yeast extract 3%, soybean peptone 3%, ammonium sulfate 4%, calcium carbonate 5%;

[0046] ③Third-stage fermentation culture, adopt the fermentation medium used in the second-stage cultivation, and cultivate in a fermenter, the inoculum size is 0.5%, the liquid volume is 80%, the rotating speed is 120rpm, the air ratio is 1:0.5, the temperature is 37°C, and the pH is 4.5 —5.0, cultivated for 24 hours to obtain a fermented liquid;

[0047] At this time, the number of viable bacteria in the fermentation broth was 3.9×10 9...

Embodiment 3

[0057] Example 3 Preparation of a Soluble Lactobacillus Probiotics

[0058] (1) Expanded culture of Lactobacillus

[0059] ①First-level culture: use conventional MRS medium as the slant medium to prepare the first-level seed slant, and cultivate at 37°C for 28 hours;

[0060] ②Secondary liquid culture, using fermentation medium, 37°C, ph4.5-6.0, shaking culture at 180rpm for 18 hours;

[0061] The formula of the fermentation medium is: glucose 1%, yeast extract 0.05%, soybean peptone 0.05%, ammonium sulfate 0.05%, calcium carbonate 0.2%;

[0062] ③Third-level fermentation culture, using the fermentation medium used in the second-level culture, cultured in a fermenter, the inoculum size is 3%, the liquid volume is 70%, the rotation speed is 90rpm, the air ratio is 1:0.4, the temperature is 37°C, and the pH is 4.5 —5.0, cultivated for 18 hours to obtain a fermented liquid;

[0063] At this time, the number of viable bacteria in the fermentation broth was 2.8×10 9 cfu / ml;

...

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Abstract

The invention pertains to the field of microbial feed additives and relates to a preparation method of a microecological preparation of lactobacillus. The preparation method has the advantages of simple requirements on technological preparation equipment, easy and convenient operation, low production cost, stable product quality and the like and is applicable to large-scale mass production. The Prepared lactobacillus powder has higher endurance capacity than other common bacterium powder and can smoothly pass though stomach and enter the intestinal tract of animals to rapidly work. In the preparation method of the microecological preparation of lactobacillus, products produced by the production technology are pretty much the same as those produced by the currently common freeze-drying technology in respect of quality, but the production cost greatly reduces. Therefore, the preparation method is beneficial to the large-scale promotion and application of the microecological preparation of lactobacillus in the feed industry.

Description

technical field [0001] The invention belongs to the field of microbial feed additives, and relates to a preparation method of a lactobacillus microecological preparation. Background technique [0002] Microecological preparation is a new type of animal health care agent that is green, safe, free of drug stress and drug residue, and is developed by bioengineering, concentration, compound compatibility and other technologies to develop microbial safety bacteria that are beneficial to animal health and animal microecological balance. , this kind of preparation can promote the micro-ecological balance of animal gastrointestinal tract or enhance non-specific immune function, thereby preventing diseases, and has the effect of improving feed conversion rate and promoting animal growth. The development and utilization of microecological preparations in my country started relatively late, and despite the rapid development, no ideal products have been released. [0003] Most of the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20A23K10/16A23K20/00
Inventor 张玳华孙占敏白玉卿姚琨贾秋英刘宇赵军
Owner 福建大北农华有水产科技集团有限公司
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