Citrobacter sp. strain DBM and method for treating acid mine drainage (AMD) using same

Abstract

The invention discloses a citrobacter sp. strain DBM and a method for treating acid mine drainage (AMD) using same. The citrobacter sp. strain DBM of the invention, collected on December 8, 2008 in the China Center for Type Culture Collection (CCTCC) has the collection number of CCTCC No.M208248. The method for treating AMD adopts the citrobacter sp. strain DBM. The obtained citrobacter sp. strain DBM by screening belongs to facultative bacteria, grows rapidly in aerobic culture, generates hydrogen sulfide in anaerobic culture and has certain tolerance to metals. In the method, the complex of sodium lactate and heavy metals is adopted to improve the biochemical availability of AMD and heavy metals in sulphate deposits are reduced by the citrobacter sp. strain DBM; metals are converted into organic complex state after complexing the AMD by the sodium lactate, which not only provides nutrition but also lowers metal toxicity; meanwhile, pH value is regulated to 6.0 without producing metal deposits, thus ensuring that the metals are fully converted into sulfide morphology, conducing to recycling.

Description

technical field [0001] The invention relates to acid mine wastewater treatment technology. Background technique [0002] Acid mine wastewater is a unique product of waste from the mining industry and its legacy abandoned mines. It is characterized by high acidity, high sulfate, high metal ions, and the release of pollutants due to the continuous oxidation of sulfide ores has become a slow process. long process. Due to the low pH and the poisoning of heavy metals, it is difficult for aquatic organisms to survive. The traditional acid mine wastewater treatment method is mainly to add lime to neutralize the precipitation. After the reaction, a large amount of gypsum and hydroxide precipitation will cause secondary pollution and require subsequent investment. Sulfate-reducing bacteria are a class of strict anaerobic prokaryotic microorganisms that widely exist in nature. They use sulfate as an electron acceptor to consume organic acids to generate sulfide, and then hydrogen su...

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Problems solved by technology

The use of sulfate reduction biological process to treat acidic wastewater is an emerging technology. However, in the actual treatment of acidic mine wastewater, problems such as high-concentration heavy metal poisoning, carbon source limitation, and low pH are often encountered, which makes wastewater treatment difficult. difficulty

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