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Method for screening strains

A strain screening and strain technology, applied in biochemical equipment and methods, microbial measurement/inspection, separation of microorganisms, etc., can solve the problem of single species, application limitation of PCB physical treatment method, and increase the difficulty of subsequent sorting process of pulverization process and other problems to achieve the effect of stable strain performance

Inactive Publication Date: 2009-09-23
SHANGHAI SECOND POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, to achieve such a fine crushing particle size will inevitably increase the difficulty of the crushing process itself and the subsequent sorting process
This also indicates that the application of PCB physical processing method will be limited
[0005] In 2007, a Chinese patent application disclosed "a comprehensive resource treatment method for waste circuit boards" (200610097382.x), although it involves the use of bacterial strains to leach Cu from waste printed circuit boards (PCBs), but pays attention to the comparison of types of PCBs single

Method used

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  • Method for screening strains
  • Method for screening strains
  • Method for screening strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 strain screening

[0041] 1 sample collection

[0042] Samples were collected from a copper mining area in my country, such as mine waste water and its sediment, the mud-water mixture at the bottom of the abandoned ore pile, the soil in the green belt at the entrance of the flotation workshop in the mining area, and the ore sludge deposited in the flotation liquid discharged from the flotation workshop. In the laboratory, the pH value of these samples and the content of various metal elements contained in them were firstly measured, and the samples with high metal content (Cu>3.0g·kg -1 ;Pb>0.5g·kg -1 ;Cd>0.2g·kg -1 ;Cr>0.5g·kg -1 ;Sn>0.3g·kg -1 ;Zn>1.5g·kg -1 ) and a sample with a lower pH value (PH1.5-2.5), perform the following operations from the sample to screen the target bacterial species.

[0043] 2 screening method

[0044] The improved Leathen liquid medium is pre-configured, and the medium consists of (units are g / L); A liquid, (NH 4 ) 2 ...

Embodiment 2

[0049] Moderate thermophilic T.f bacteria B leaching Cu on copper clad laminate particles with high metal content

[0050] 2.1 Granule preparation

[0051] Preparation of copper-clad laminate particles: The crushed PCB particles on a pulverizer (Xiaoma Machinery Shanghai Co., Ltd., model GSC) pass through a standard sieve. The selected standard sieve has a sieve size of 0.50mm and 1.00mm. Particles are subpackaged, and fully mixed before subpackaging to ensure the uniformity of the sample, with 0.50-1.00mm as the test particle.

[0052] 2.2 Experimental design

[0053] The liquid culture medium of T.f bacterium is the improved Leatheren medium composition (g / L): A liquid, (NH 4 ) 2 SO 4 0.15,K 2 HPO 4 0.05, MgSO 4 ·7H 2 O 0.05, KCl 0.05, Ca(NO 3 ) 2 0.01, pH value 2.5, sterilized by moist heat; liquid B, ferrous sulfate 15, filter sterilized; add liquid B after cooling liquid A, mix well and set aside. Divide 45ml of the above-mentioned culture medium A into 150ml...

Embodiment 3

[0060] Experiment on the leaching of metals from copper-clad boards containing green oil by moderately thermophilic T.f bacteria C

[0061] 3.1 Granule preparation

[0062] Preparation of copper-clad laminate particles containing green oil: The PCB particles pulverized on a pulverizer pass through a standard sieve, and the particles with a higher metal content of 0.50-1.00mm are selected as the test particles.

[0063] 3.2 Experimental design

[0064] Same as 2.2.

[0065] After culturing for 7 days, samples were taken to analyze the Cu content in the solution.

[0066] 3.3 Determination method

[0067] Same as 2.3

[0068] 3.4 Results

[0069] The leaching rate of Cu on copper-clad boards containing green oil inoculated with moderate thermophilic T. bacterium C increased with the prolongation of culture time. By the seventh day, the Cu leaching rate on copper-clad boards containing green oil reached 92%. The results show that the green oil on the PCB will not poison the...

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Abstract

The invention provides a method for screening strains, comprising the following steps: 1. collecting samples with Cu more than or equal to 3.0g.kg<-1> and pH being 1.5-2.5 in a metal ore field; 2. carrying out accumulation culture on the strains in the samples and separating the same; 3. domesticating the strains separated in step 2 and separating to obtain the finally survived strains. The strains screened by the method of the invention can efficiently leach metals such as Cu, Pb, Zn, Ni and the like out of different PCB grains of various natures.

Description

technical field [0001] The invention relates to a biotechnology method, in particular to a strain screening method. Background technique [0002] With the rapid development of information science and technology, the output of solid waste from electronic products has increased rapidly, and electronic waste is becoming a big problem that plagues the world. People usually refer to household appliances and electronic products such as televisions, refrigerators, washing machines, air conditioners, computers, mobile phones, game consoles, radios, tape recorders that are eliminated or scrapped in daily life as waste electrical and electronic equipment (WEEE, referred to as waste electronic equipment). things). In Europe, 6 million tons of WEEE were generated in 1998, increasing at a rate of at least 3-5% per year. In the United States, by 2004, 315 million computers will enter the end of life. In my country, at least 5 million TVs, 4 million refrigerators, and 6 million washing ...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12Q1/02
Inventor 白建峰王景伟徐金球张承龙
Owner SHANGHAI SECOND POLYTECHNIC UNIVERSITY