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Vegetable proteins extracted from P.octandra, preparation method and application thereof

A technology of plant protein and extraction method, which is applied in the field of pharmaceuticals, can solve the problems of no treatment method, etc., and achieve the effect of definite curative effect and clear ingredients

Inactive Publication Date: 2009-09-30
林俊生 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous emergence of various vaccines, some viral diseases have been effectively prevented, but there is no effective treatment.

Method used

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  • Vegetable proteins extracted from P.octandra, preparation method and application thereof
  • Vegetable proteins extracted from P.octandra, preparation method and application thereof
  • Vegetable proteins extracted from P.octandra, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Extract crude vegetable protein, fine protein and protamine from ink grass (P.octandra)

[0037] 1). Homogenate: Collect and weigh 1 kg of the spring leaves of Mocao, add 2 liters of 10mM phosphate buffer pH6.5, mash it with T50 Basic ULTRA-TURRAX to form a homogenate, filter and keep the filtrate for later use.

[0038] 2). Ammonium sulfate two-step precipitation: a). Take 1.95 liters of the filtrate obtained in step 1) and add 1.05 liters of ammonium sulfate saturated solution slowly and uniformly in proportion to make it 35% ammonium sulfate saturation; stand at 10°C for 1 Hour. Centrifuge precipitation (20°C, 30 minutes, 3000g centrifugal force) to retain the supernatant; b). Add solid ammonium sulfate to the obtained supernatant under stirring to make it 100% ammonium sulfate saturation, and continue stirring at 20°C for 15 hours. Precipitate by centrifugation (20°C, 30 minutes, 3000g centrifugal force), carefully discard the supernatant and retain t...

Embodiment 2

[0049] Embodiment 2: Extract crude vegetable protein, fine protein and protamine from ink grass (P.octandra)

[0050] 1). Homogenate: Collect and weigh 1 kg of the spring leaves of Mocao, add 2 liters of 8mM phosphate buffer pH5, mash with T50Basic ULTRA-TURRAX to form a homogenate, filter and keep the filtrate for later use.

[0051] 2). Ammonium sulfate two-step precipitation: a). Take 1.95 liters of the filtrate obtained in step 1) and slowly add saturated ammonium sulfate solution in proportion to the saturated ammonium sulfate solution at a constant speed in a stirring state to make the saturation of ammonium sulfate 30%; stand at 4°C for 0.5 hours. Centrifuge precipitation (4°C, 30 minutes, 3000g centrifugal force) to retain the supernatant; b). Add solid ammonium sulfate to the obtained supernatant under stirring to make it 100% ammonium sulfate saturation, and continue stirring at 4°C for 2 hours. Precipitate by centrifugation (4°C, 30 minutes, 3000g centrifugal force)...

Embodiment 3

[0062] Embodiment 3: Extract crude vegetable protein, fine protein and protamine from Chinese ink grass (P.octandra)

[0063] 1). Homogenate: Collect and weigh 1 kg of the spring leaves of Mocao, add 2 liters of 12mM phosphate buffer pH8, mash it with T50Basic ULTRA-TURRAX to form a homogenate, filter and keep the filtrate for later use.

[0064] 2). Ammonium sulfate two-step precipitation: a). Take 1.95 liters of the filtrate obtained in step 1) and add the saturated ammonium sulfate solution slowly and uniformly in proportion to 40% ammonium sulfate saturation under stirring state; stand at 20°C for 2 hours. Centrifuge precipitation (20°C, 30 minutes, 3000g centrifugal force) to retain the supernatant; b). Add solid ammonium sulfate to the obtained supernatant under stirring to make it 100% ammonium sulfate saturation, and continue stirring at 20°C for 18 hours. Precipitate by centrifugation (20°C, 30 minutes, 3000g centrifugal force), carefully discard the supernatant and r...

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Abstract

The invention discloses vegetable proteins extracted from P.octandra, wherein the molecular weights of the vegetable proteins are between 27 and 38 kDa. The invention also discloses a method for extracting the vegetable proteins, which comprises the following steps that leaves of the P.octandra are prepared into a homogenate, the filtration is performed, and different stages of extracts are selected to prepare corresponding preparations according to different administration routes through ammonium sulfate two-step precipitation, dialysis and chromatographic purification. The invention also discloses an anti-virus medicament containing the vegetable proteins. The anti-virus medicament has clear main constituents and exact therapeutic effect, and is particularly suitable for the prevention and treatment of viral diseases.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a plant protein extracted from P.octandra, a preparation method thereof, an antiviral drug containing the same and an application thereof. Background of the invention [0002] Inkgrass (P.octandra) is a perennial herbaceous South Pacific plant, usually about 1 meter high and about 1 meter wide. Stems are smooth, green or red. The leaves are 5-16 cm long, oval in shape, with pointed tips. Small white flowers arranged in tight clusters. Ripe fruit is dark purple, about 5mm in diameter, arranged in tight clusters. So far, there is no report about preparing antiviral drugs from Mocao. [0003] Viral diseases are the main infectious diseases popular at home and abroad, especially in recent years, it is well known that SARS caused by coronavirus and bird flu caused by influenza virus have brought serious harm to animals and humans. The continuous emergence of various vaccines has effective...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/30C07K1/16A61K38/16A61K9/00A61K9/08A61K9/12A61K9/14A61P31/12
Inventor 林俊生许瑞安刁勇
Owner 林俊生
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