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A preparing method and application of malaytea scurfpea fruit total glycosides extract

A technology of glycoside extract and psoralen, which is applied in the field of medicine, can solve the problems of not yet obtaining psoralen glycosides, etc., and achieve the effect of significant immune enhancement and simple method

Inactive Publication Date: 2011-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Psoralen contains a variety of active ingredients, and the glycosides represented by psoralen and isopsoralen are one of its important active ingredients, but there is no report of obtaining psoralen in the current literature and patents

Method used

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  • A preparing method and application of malaytea scurfpea fruit total glycosides extract
  • A preparing method and application of malaytea scurfpea fruit total glycosides extract
  • A preparing method and application of malaytea scurfpea fruit total glycosides extract

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Embodiment 1

[0020] The HPLC analysis and content determination method of embodiment 1 psoralen and isopsoralen

[0021] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 250mm, 5 μ m); Adopt gradient elution, mobile phase A phase is 0.1% glacial acetic acid aqueous solution, mobile phase B phase is the acetonitrile solution containing 0.1% glacial acetic acid; Gradient The elution procedure is as follows: at 0 minutes, the mobile phase is 95% A, 5% B; at 20 minutes, the mobile phase is 70% A, 30% B; at 25 minutes, the mobile phase is 50% A , 50% B; at 30 minutes, the mobile phase was 5% A, 95% B. Solution flow rate 1.0mL·min -1 ; The detection wavelength is 246nm; the column temperature is 30°C, and the content of psoralen and isopsoralen can be determined. see figure 1 , 1 in the figure is isopsoralen, and 2 is psoralen.

Embodiment 2

[0023] Take 1 kg of psoraleae, dry at 50°C for 6 hours, add 8 times the amount of water, heat and reflux for 2 hours in a slightly boiling state, extract the extract, filter it, concentrate under reduced pressure, and precipitate with alcohol, adjust the alcohol concentration to 80%, take the supernatant, and concentrate Add ethyl acetate to extract after reaching no alcohol, separate the water layer, apply macroporous resin, wash with water, discard, and continue to elute with 30% ethanol aqueous solution. The eluate was collected, concentrated under reduced pressure until alcohol-free, and freeze-dried to obtain total psoralen glycosides. As determined by the method of Example 1, the content of psoralen in the total psoralen was 38%, and the content of isopsoralen was 42%.

Embodiment 3

[0025] Take 1 kg of psoraleae, dry at 50°C for 6 hours, add 8 times the amount of water and heat to reflux for 2 hours, then filter the extract, concentrate under reduced pressure, and precipitate with alcohol, adjust the alcohol concentration to 70%, take the supernatant, and concentrate Add ethyl acetate to extract after reaching no alcohol, separate the water layer, apply macroporous resin, wash with water, discard, and continue to elute with 50% ethanol aqueous solution. The eluate was collected, concentrated under reduced pressure until alcohol-free, and freeze-dried to obtain total psoralen glycosides. Measured according to the method of Example 1, the content of psoralen in the total psoralen is 40%, and the content of isopsoralen is 42%.

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Abstract

The present invention provides a method of preparing malaytea scurfpea fruit total glycoside extract, including: using water to extracting the malaytea scurfpea fruit, concentrating the extract and then performing alcohol deposition, using small polar organic reagent to extract the supernatant, using macroporous resin for purifying the aqueous layer after extraction to obtain the total malaytea scurfpea fruit glycoside extract, wherein, the content of the total malaytea scurfpea fruit glycosides is more than 80%, which contains 40-50% of malaytea scurfpea fruit glycosides, 40-50% of isopsoralen glycosides by HPLC content measuring. The malaytea scurfpea fruit total glycoside extract provided by the present invention is authenticated by pharmacological experiments that it can fight againstcyclophosphamide-induced leukopenia, has a significant role in immune enhancement, and can be applied in the medicament for increasing leucocyte. The inventive method obtains malaytea scurfpea fruit total glycosides from the Chinese medicine malaytea scurfpea fruit, which is simple, convenient, economic, stable, easy to operate, and is suitable for industrial mass production.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for obtaining total psoralen extract from the traditional Chinese medicine psoraleae through extraction and refining, and the medicinal use of the extract. Background technique [0002] At present, Chinese herbal medicine has a certain market all over the world. With the increase of people's awareness of health requirements and the aging of the population, sub-health status, people are more eager to return to nature, and use pure natural medicine with high degree of treatment, To prevent problems that cannot be solved by some chemically synthesized drugs, the application of natural herbal medicines is beyond the background of its original national traditional culture. It has become the goal pursued by pharmaceutical companies all over the world to seek medicines with low side effects and high quality and low price from natural medicines. The European Community has ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/487A61P7/00A61P37/04A61K131/00
Inventor 程翼宇瞿海斌吴斌
Owner ZHEJIANG UNIV
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