Fermentation lactobacillus preparation

A technology for fermenting Lactobacillus and preparations, which is applied in the direction of bacteria, animal feed, additional food elements, etc., and can solve problems such as excessive strains, high cultivation costs, and unstable product quality

Active Publication Date: 2009-10-14
GUANGZHOU LEADER BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned additives need to include four kinds of lactobacilli, which require high cultivation costs, and too many strains also make the product quality unstable

Method used

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  • Fermentation lactobacillus preparation
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Inoculate Lactobacillus fermentum CGMCC No.0843 in glucose nutrient broth, its components are: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2.5g of glucose, 15g of agar, 1000ml of distilled water, and cultivate in an ordinary incubator at 37 degrees for 18 - After 24 hours, serially dilute to obtain a concentration of 10 7 -10 10 cfu / g of the culture solution.

[0048] By weight, mix 1 part of the strain solution containing Lactobacillus fermentum CGMCC No.0843 and 30 parts of calcium hydrogen phosphate to obtain the Lactobacillus fermentum preparation.

Embodiment 2

[0050] Lactobacillus fermentum CGMCC No.0843 was inoculated and cultivated in Rogosa broth, and its components included: 10g tryptone, 5g yeast extract powder, 10g glucose, 5g arabinose, 5g sucrose, 15g sodium acetate, 2g ammonium citrate, 6g potassium dihydrogen phosphate, 0.30g anhydrous magnesium sulfate, 0.12g manganese sulfate, 0.03g ferrous sulfate, 1g Tween-80, 1000ml distilled water, and adjust the pH to 5.4 with acetic acid; put it in an ordinary incubator at 37 degrees for 18-24 Hours later, serially dilute to obtain a concentration of 10 7 -10 10 cfu / g of the culture solution.

[0051] By weight, 10 parts of a strain solution containing Lactobacillus fermentum CGMCC No.0843 and 150 parts of calcium phosphate were mixed, and the calcium phosphate was micronized in advance to obtain a Lactobacillus fermentum preparation.

Embodiment 3

[0053] The Lactobacillus fermentum CGMCC No.0843 cultivated in Example 1 was placed in a fermenter for primary fermentation, and after 18-24 hours of cultivation, it was serially diluted to obtain a concentration of 10 7 -10 10 cfu / g of the culture solution. The medium components of the fermenter are 20 parts of bean cake powder, 15 parts of corn flour, 10 parts of molasses, and an appropriate amount of water is added. The ratio of carbon to nitrogen and phosphorus in the medium is 100:5:0.5.

[0054] By weight, 5 parts of strain liquid containing Lactobacillus fermentum CGMCC No.0843, 80 parts of light calcium carbonate, and 20 parts of calcium phosphate are mixed, and light calcium carbonate and calcium phosphate are all processed through micronization in advance (90% The particle size of the powder is less than 100 μm, the average particle size is less than 50 μm, and the average particle size is 50% of the particle size of the powder), and the fermented Lactobacillus pre...

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Abstract

The invention provides a fermentation lactobacillus preparation which comprises the following components by weight part: 1-10 of strain liquid of fermentation lactobacillus CGMCC No.0843 and 30-150 of mineral carrier; the optimal composition contains 5-8 of strain liquid of fermentation lactobacillus CGMCC No.0843 and 60-100 of strain liquid carrier; and the concentration of the strain liquid is 10-10cfu/g. The preparation can be used for regulating the balance of microecology in the intestine of animals, promoting the growth of animals and improving the feed conversion rate at the same time, thereby having the function of enhancing nonspecific immune function to prevent diseases; and meanwhile, by screening the prescriptions, the preparation has low cost and stable product quality.

Description

technical field [0001] The invention relates to a fermenting lactobacillus preparation, in particular to a preparation containing fermenting lactobacillus CGMCC No.0843. Background technique [0002] Since the discovery that feed antibiotics can play a growth-promoting role by affecting the initial colonization of intestinal microorganisms in the host, the application of antibiotics has produced huge economic benefits in animal husbandry. However, due to the extensive use of antibiotics, the accompanying negative effects have become increasingly prominent, manifested as the development of drug resistance by pathogenic microorganisms and drug residues in animal products, thereby causing potential harm to human health. [0003] In recent years, the mad cow disease in the UK, the dioxin incident in Belgium and the clenbuterol poisoning incident in Hong Kong have made consumers increasingly concerned about the safety of animal products. The increase in drug-resistant pathogenic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20A23K10/18A23K20/26A23K20/28
Inventor 谯仕彦潘宝海宋青龙王旭俞海峰
Owner GUANGZHOU LEADER BIO TECH
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