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Separating and refining method of arabitol in fermentation liquor

The technology of arabinitol and refining method is applied in the field of separation and refining of arabitol in fermentation broth, which can solve the problems of difficult handling, complicated process and low purity, and achieve increased economic benefits, increased yield, improved yield and The effect of purity

Inactive Publication Date: 2012-05-23
FUTASTE PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

D-arabitol can be produced by fermenting D-glucose with yeast (H.Wouter Wisselink et al., 2007), and the conversion rate can reach 50%-60%, but the composition of the fermentation broth is complex, which brings difficulties to subsequent processing
In particular, among the by-products, glycerin occupies a large proportion, which increases the difficulty of decolorization and concentration of the fermentation broth
In addition, the traditional method of extracting glycerin from fermentation broth is the method of vacuum distillation, the yield of glycerin is only 60%, the purity is low, the removal of pigment is difficult, and the follow-up process is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Take 30 liters of fermented liquid containing D-arabinitol obtained by fermenting D-glucose with yeast, centrifuge in a centrifuge at 4500 rpm for 10 minutes, remove the thallus to obtain a fermented liquid without thallus, dry The substance content is 7%, the pH value is 4.5, the fermented liquid is heated to above 90°C, kept for 10 minutes, the pH value is adjusted to 2, and activated carbon is added according to 1% of the dry matter, stirred evenly, heated to 75°C, kept for 1 hour, Vacuum suction filtration to remove activated carbon; the decolorized fermentation broth is ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 7000, the feed rate is 0.5 liters / min, and the pressure is 0.1 kg for further clarification; the above-treated fermentation broth is heated at 75 ° C , using a rotary evaporator to concentrate to a refractive index of 20%, and determined by high performance liquid chromatography, the purity of arabitol is ...

Embodiment 2

[0015] Example 2: Take 30 liters of fermented liquid containing D-arabinitol obtained by fermenting D-glucose with yeast, and centrifuge it in a centrifuge at 6000 rpm for 20 minutes to remove the cells to obtain a fermented liquid without cells, dry The substance content is 7%, the pH value is 4.5, the fermented liquid is heated to above 90°C, kept for 30 minutes, the pH value is adjusted to 4, activated carbon is added according to 5% of the dry matter, stirred evenly, heated to 90°C, and kept for 3 hours, Vacuum suction filtration to remove activated carbon; the decolorized fermentation broth was ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 7000, the feed rate was 3 liters / min, and the pressure was 0.4 kg for further clarification; the above-treated fermentation broth was heated at 75°C , using a rotary evaporator to concentrate to a refractive index of 70%, and determined by high performance liquid chromatography, the purity of arabit...

Embodiment 3

[0016] Example 3: Take 30 liters of fermented liquid containing D-arabitol obtained by fermenting D-glucose with yeast, centrifuge in a centrifuge at 5000 rpm for 15 minutes, remove the cells to obtain a fermented liquid without cells, dry The substance content is 7%, the pH value is 4.5, the fermentation broth is heated to above 90°C, kept for 20 minutes, the pH value is adjusted to 3, 3% of the dry matter is added with activated carbon, stirred evenly, heated to 80°C, kept for 2 hours, Vacuum suction filtration to remove activated carbon; the decolorized fermentation broth was ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 7000, the feed rate was 2 liters / min, and the pressure was 0.25 kg for further clarification; the above-treated fermentation broth was heated at 75°C , using a rotary evaporator to concentrate to a refractive index of 45%, as determined by high performance liquid chromatography, the purity of arabitol is 75%, and the pu...

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Abstract

The invention discloses a separating and refining method of arabitol in fermentation liquor, belonging to the field of arabitol preparation. The preparation method comprises the steps of heat treatment, decolorization, ultrafiltration, chromatographic resolution and final crystallization and drying. The method improves the yield and purity of the arabitol product. Byproducts are dehydrated and concentrated to obtain crude glycerine and the yield of glycerine of the byproducts is increased, thereby increasing the economic benefits.

Description

technical field [0001] The invention relates to the field of arabitol preparation, in particular to a method for separating and refining arabitol in a fermentation broth. Background technique [0002] D-arabitol has a wide range of uses and can be used as a sweetener, food additive, activator for foaming materials, stabilizer for developing materials, and many pharmaceutical intermediates. Glycerin is also an important chemical raw material and food additive. Chemically, it can be used to make glyceryl trinitrate or alkyd resin, etc. It can be used as sweetener and humectant in food processing industry. [0003] Both arabitol and glycerol can be produced by biological fermentation. Due to similar biological metabolic pathways, the two substances often appear in the same fermentation broth, and one appears as a by-product of the other. D-arabinitol can be produced by fermenting D-glucose with yeast (H.Wouter Wisselink et al., 2007), and the conversion rate can reach 50%-60%...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C31/18C07C29/76C07C31/22B01D15/08
Inventor 王星云黄伟红杜瑞锋李强陈红军
Owner FUTASTE PHARM CO LTD