Technique for extracting L-tryptophan in fermentation liquor

Abstract

The invention pertains to the technical field of biological engineering and particularly discloses a technique for extracting L-tryptophan in fermentation liquor. The fermentation liquor is filtered by a ceramic film, filtered liquid is adsorbed by polymeric adsorbent and resolved by ammonia, and L-tryptophan product is obtained through decolorizing, filtering and drying resolved liquid sequentially. The technique is simple and environment-friendly. The L-tryptophan extracted by the technique has both high yield and stable quality.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an extraction process of L-tryptophan in a fermentation broth. Background technique [0002] In the process of traditional fermentation to produce L-tryptophan, the extraction process of L-tryptophan is as follows: figure 1 shown. The disadvantages of this technological process are: due to the low solid content of the fermentation liquid (about 20%), the plate and frame filtration speed is slow, the filtrate contains many impurities, and the plate and frame are labor-intensive and complicated to load and unload, which is easy to form a bottleneck in the production process. The effect is not ideal; due to the large amount of impurities in the solution filtered by the plate and frame, the resin adsorption equivalent is reduced, the resin utilization rate is low, the resin analysis and regeneration frequency are frequent, the resin treatment cost is high, and the...

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Problems solved by technology

The disadvantages of this technological process are: due to the low solid content of the fermentation liquid (about 20%), the plate and frame filtration speed is slow, the filtrate contains many impurities, and the plate and frame are labor-intensive and complicated to load and unload, which is easy to form a bottleneck in the production process. The effect is unsatisfactory; due to the large amount of impurities in the solution filtered by the plate and frame, the resin adsorption equivalent is reduced, the resin utilization rate is low, the resin analysis and regeneration frequency are frequent, the resin treatment cost is high, and the product quality cannot be guaranteed; now a resin volume is 1.3 tons, the analysis solution is about 7 tons, a batch of materials requires 3.5 resin columns, about 4.5 tons of resin is used, and the volume of the batch analysis solution is more than 25 tons. The volume of the analysis solution is too large, resulting in high vacuum film concentration pressure; The crude product dissolves in a small amount (the solubility is about 3%, and 300 kg of product needs to be dissolved in 10 tons of water), resulting in a large amount of dissolved liquid and a large amount of activated carbon, resulting in high pressure on the secondary concentration equipment and forming a bottleneck in the production process

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