Technique for extracting L-tryptophan in fermentation liquor

An extraction process and tryptophan technology, applied in the field of bioengineering, can solve the problems of high pressure of secondary concentration equipment, slow plate and frame filtration, and high pressure of vacuum film concentration, so as to ensure product quality and content, and process environmental pollution. Small, product throughput reduction effect

Inactive Publication Date: 2009-10-28
河南孟成生物药业股份有限公司
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this technological process are: due to the low solid content of the fermentation liquid (about 20%), the plate and frame filtration speed is slow, the filtrate contains many impurities, and the plate and frame are labor-intensive and complicated to load and unload, which is easy to form a bottleneck in the production process. The effect is unsatisfactory; due to the large amount of impurities in the solution filtered by the plate and frame, the resin adsorption equivalent is reduced, the resin utilization rate is low, the resin analysis and regeneration frequency are frequent, the resin treatment cost is high, and the product quality cannot be guaranteed; now a resin volume is 1.3 tons, the analysis solution is about 7 tons, a batch of materials requires 3.5 resin columns, about 4.5 tons of resin is used, and the volume of the batch analysis solution is more than 25 tons. The volume of the analysis solution is too large, resulting in high vacuum film concentration pressure; The crude product dissolves in a small amount (the solubility is about 3%, and 300 kg of product needs to be dissolved in 10 tons of water), resulting in a large amount of dissolved liquid and a large amount of activated carbon, resulting in high pressure on the secondary concentration equipment and forming a bottleneck in the production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Technique for extracting L-tryptophan in fermentation liquor
  • Technique for extracting L-tryptophan in fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Such as figure 2 The process flow diagram for the extraction of L-tryptophan in the fermented liquid of the present invention shown, fermented liquid 1000ml (fermented liquid specific gravity 0.98, solid matter percentage content 18%, wherein L-tryptophan 250g) passes through Al 2 o 3 The ceramic membrane with a mass percentage of 99% is used for filtration, the pore size of the ceramic membrane is 0.1 μm, and the filtration flux is 10l / m 2 h, the solid-liquid mixture filtered by ceramic membrane (the main component is biological protein, solid content 35%) is filtered by plate and frame, dried and used as protein feed. Adsorption, adsorption flow 3m 3 / h, the batch adsorption time is 8h, and then analyze with 0.5mol / l ammonia water, the analysis flow rate is twice the volume of the resin per hour, and the analysis solution is sent to the basic anion resin D315 column for decolorization, and the decolorization solution is first filtered by nanofiltration Membrane (t...

Embodiment 2

[0026] The difference from Example 1 is that the saturated solution obtained after ultrafiltration is adjusted to a pH of 2.0 with sulfuric acid with a mass concentration of 20% to induce crystallization of L-tryptophan, and then dried to obtain 235.5 g of L-tryptophan product, the product The yield is 94.2%, and the content is 98.6%.

Embodiment 3

[0028] Such as figure 2 The extraction process flow diagram of L-tryptophan in the fermented liquid of the present invention shown, fermented liquid 1000ml (fermented liquid specific gravity 0.98, solid matter percentage content 20%, wherein L-tryptophan 250g) passes through Al 2 o 3 The ceramic membrane with a mass percentage of 99% is used for filtration. The pore size of the ceramic membrane is 0.3 μm, and the filtration flux is 30l / m 2 h, the solid-liquid mixture filtered by ceramic membrane (the main component is biological protein, the solid content is 40%) is filtered by plate and frame, dried and used as protein feed, and the filtered clear liquid is used as strong acid cation adsorption resin JK008 column Adsorption, adsorption flow 3.5m 3 / h, the batch adsorption time is 10h, and then analyze with 1mol / l ammonia water, the analysis flow rate is 2.5 times the volume of the resin per hour, and the analysis solution is sent to the basic anion resin D305 column for de...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention pertains to the technical field of biological engineering and particularly discloses a technique for extracting L-tryptophan in fermentation liquor. The fermentation liquor is filtered by a ceramic film, filtered liquid is adsorbed by polymeric adsorbent and resolved by ammonia, and L-tryptophan product is obtained through decolorizing, filtering and drying resolved liquid sequentially. The technique is simple and environment-friendly. The L-tryptophan extracted by the technique has both high yield and stable quality.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an extraction process of L-tryptophan in a fermentation broth. Background technique [0002] In the process of traditional fermentation to produce L-tryptophan, the extraction process of L-tryptophan is as follows: figure 1 shown. The disadvantages of this technological process are: due to the low solid content of the fermentation liquid (about 20%), the plate and frame filtration speed is slow, the filtrate contains many impurities, and the plate and frame are labor-intensive and complicated to load and unload, which is easy to form a bottleneck in the production process. The effect is not ideal; due to the large amount of impurities in the solution filtered by the plate and frame, the resin adsorption equivalent is reduced, the resin utilization rate is low, the resin analysis and regeneration frequency are frequent, the resin treatment cost is high, and the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D209/20B01D71/02B01D61/14A23K1/00A23K10/12A23K20/142A23K20/147
Inventor 孟军虎王进全武涛
Owner 河南孟成生物药业股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap