A method for producing xylooligosaccharides from cottonseed hulls

A technology of xylooligosaccharides and cottonseed hulls, applied in the field of production of xylooligosaccharides, can solve the problems of complex composition, weak functionality, and no functionality of bifidobacteria value-added factors, and achieve short processing time and low production costs , The effect of shortening the processing time

Inactive Publication Date: 2011-12-28
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dilute acid hydrolysis products are oligosaccharides with a degree of polymerization of 2 to 15. The composition is very complex and the functionality is not strong, because the most functional xylooligosaccharides are xylobiose and xylotriose, xylotetraose to xyloheptose There is also functionality, xylo-oligosaccharides with a degree of polymerization greater than 7 no longer have the functionality of bifidobacteria growth factors

Method used

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  • A method for producing xylooligosaccharides from cottonseed hulls
  • A method for producing xylooligosaccharides from cottonseed hulls
  • A method for producing xylooligosaccharides from cottonseed hulls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: Weigh a certain amount of cottonseed hulls, soak in 0.2% (m / v) sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreat at room temperature for 24 hours, and rinse with water for 3 times, which can effectively remove the pigment substances in the raw materials .

[0029] Step 2: The wet cottonseed hulls are then treated with high-pressure steam at 180°C for 15 minutes.

[0030] Step 3: Add 1.0% (m / m) xylanase after cooling to 50°C with water (based on the dry weight of cottonseed hulls, xylanase is the commercial enzyme Novozymes Pentopan Mono BG), at a temperature of 45-50°C Under the condition of pH 4.0-pH 5.5, the enzyme was hydrolyzed for 36 hours under the condition of slow stirring or shaking.

[0031] Step 4: After the enzymolysis solution is boiled for 10 minutes to kill the enzyme, it is then filtered (filtered by a plate and frame filter) and vacuum concentrated (three-effect or four-effect vacuum concentration) to a sugar concentration o...

Embodiment 2

[0033] Step 1: Weigh a certain amount of cottonseed hulls, soak in 0.5% (m / v) sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreat at room temperature for 20 hours, and rinse with water for 5 times, which can effectively remove the pigment substances in the raw materials .

[0034] Step 2: The wet cottonseed hulls are then treated with high-pressure steam at 190°C for 7 minutes.

[0035] Step 3: Add 2.5% (m / m) xylanase after cooling to 50°C with water (based on the dry weight of cottonseed hulls, xylanase is the commercial enzyme Novozymes Pentopan Mono BG), at a temperature of 45-50°C And under the condition of pH4.0-pH5.5, after 24 hours of enzymatic hydrolysis under the condition of slow stirring or shaking.

[0036] Step 4: Boil the enzymatic solution for 10 minutes to kill enzymes, then filter and vacuum concentrate to a sugar concentration above 75% (m / m) to obtain xylooligosaccharide syrup.

Embodiment 3

[0038] Step 1: Weigh a certain amount of cottonseed hulls, soak in 0.3% (m / v) sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreat at room temperature for 24 hours, and rinse with water for 3 times, which can effectively remove the pigment substances in the raw materials .

[0039] Step 2: The wet cottonseed hulls are then treated with high-pressure steam at 185°C for 10 minutes.

[0040] Step 3: Add 1.0% (m / m) xylanase after cooling to 50°C with water (based on the dry weight of cottonseed hulls, xylanase is the commercial enzyme Novozymes Pentopan Mono BG), at a temperature of 45-50°C And under the condition of pH4.0-pH5.5, after 36 hours of enzymatic hydrolysis under the condition of slow stirring or shaking.

[0041] Step 4: Boil the enzymatic solution for 10 minutes to kill enzymes, then filter and vacuum concentrate to a sugar concentration above 75% (m / m) to obtain xylooligosaccharide syrup.

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Abstract

A method for producing xylooligosaccharides by using cottonseed hulls, using cottonseed hulls as raw materials, soaking in dilute sulfuric acid solution with a solid-to-liquid ratio of 1:10, pretreating at room temperature for 20-24 hours, and then passing through high-pressure steam at 180-190°C Treat for 7-15 minutes, add 1.0-2.5% xylanase after the water is cooled to 50°C, and enzymolyze 24-36 hours under the conditions of temperature 45-50°C and pH4.0-pH5.5 under slow stirring or shaking conditions Hour. The enzymolysis solution is boiled for 10 minutes to kill enzymes, and then filtered and concentrated in vacuum until the sugar concentration is above 75% to obtain the xylooligosaccharide syrup. The present invention is characterized in that: Cottonseed hulls with more xylose content in raw xylan are used as raw materials; pretreatment effectively removes pigment substances; high pressure treatment can significantly shorten the time; the production process of xylooligosaccharides is simple and practical, and the production cost is relatively low. Low; the xylo-oligosaccharide production process and method of the present invention have good industrial application prospects.

Description

technical field [0001] The invention relates to a production method of xylooligosaccharides, in particular to a method for producing xylooligosaccharides using cottonseed hulls as raw materials. Background technique [0002] my country is a big cotton producing country. In 2007, the country's cotton planting area was 82.65 million mu, and the cottonseed output was about 12 million tons, ranking first in the world. Based on this, it is estimated that the annual output of cottonseed hulls in my country is about 5.4 million tons. From the perspective of the utilization of cottonseed hulls in my country, except for a small amount of them used for the cultivation of edible fungi and the production of boards, a large amount of cottonseed hulls are discarded, and their biomass utilization rate is extremely low. The content of xylan in cottonseed hull is about 28%, which is abundant. Compared with other xylan-rich raw materials such as corncobs, cottonseed hulls have the characte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 丁长河洪丰陈复生李里特王罗琳
Owner HENAN UNIVERSITY OF TECHNOLOGY
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