Method for preparing natural ossein

A technology of natural bone collagen and bone mud, applied in the field of extraction and purification of natural bone collagen, can solve the problems of less than 10% utilization rate, environmental pollution, etc., and achieve the effect of high extraction rate

Active Publication Date: 2009-11-04
CHINA MEAT RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] my country is the largest meat producing country in the world. Livestock and poultry bones account for 20%-30% of animal weight, and nearly 20 million tons of lives...

Method used

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  • Method for preparing natural ossein
  • Method for preparing natural ossein
  • Method for preparing natural ossein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 purifies natural bovine bone collagen powder from bovine bone

[0046]It is more common to extract natural collagen from animal skin, but it is rare to extract it from bone. It involves high fat and inorganic content, difficult extraction rate and purification rate, etc., especially bovine bone.

[0047] The present embodiment purifies natural bovine bone collagen powder from the bovine rod bone, and is prepared by the following steps:

[0048] (1) Cleaning: Use 1000g of fresh beef rod bone as raw material, and wash with water to ensure that the raw material bone meets hygienic requirements.

[0049] (2) Powerful bone breaking: Use a bone breaker to carry out strong bone breaking on the cleaned bovine stick bones. In order to ensure the smooth discharge of fresh bones, 300g of ice water is added during crushing, and the particle size after coarse crushing is ≤1mm.

[0050] (3) Fine crushing: The material after strong bone crushing is ground in two stages ...

Embodiment 2

[0063] The present embodiment purifies natural collagen powder from pig bones, and is prepared by the following steps:

[0064] (1) Cleaning: 1000g of fresh pork bone is used as raw material, and washed with water to ensure that the raw material bone meets hygienic requirements.

[0065] (2) Powerful bone breaking: Use a bone breaker to carry out strong bone breaking on the cleaned pig bones. In order to ensure the smooth discharge of fresh bones, 400g of ice water is added during crushing, and the particle size after coarse crushing is ≤1mm.

[0066] (3) Fine crushing: The material after strong bone crushing is ground in two stages with a bone mud mill. The first stage has a large gap and the second stage has a small gap. When the temperature is ≤10°C, the amount of added ice chips is 30% of the fresh bone weight. After being finely pulverized, bone paste with a particle size of 80 mesh was obtained.

[0067] (4) Degreasing and removing inorganic substances: degreasing is ...

Embodiment 3

[0075] Present embodiment purifies natural collagen powder from chicken bone, is made by following steps:

[0076] (1) Cleaning: 1000g of chicken bone is used as raw material, and washed with water to ensure that the raw material bone meets hygienic requirements.

[0077] (2) Powerful bone breaking: Use a bone breaker to perform strong bone breaking on the cleaned chicken bones. In order to ensure the smooth discharge of fresh bones, 350g of ice water is added during crushing, and the particle size after coarse crushing is ≤1mm.

[0078] (3) Fine crushing: The material after strong bone crushing is ground in two stages with a bone mud mill. The first stage has a large gap and the second stage has a small gap. When the temperature is ≤10°C, the amount of added ice chips is 35% of the fresh bone weight. After being finely pulverized, bone paste with a particle size of 100 mesh was obtained.

[0079] (4) Degreasing and removing inorganic substances: degreasing is carried out b...

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Abstract

The invention provides a method for preparing natural ossein, which adopts extraction and purification from beasts and birds bones and comprises the following steps: cleaning; crushing; degreasing and inorganic substance removal; acid soaking; enzyme hydrolysis; separation and purification; and freeze drying. The natural ossein prepared by the method can keep a complete collagen triple helix structure so that the biological activity is retained, the purity can reach more than 90 percent, and the natural ossein has higher extraction yield and can be widely applied in the fields of foods, cosmetics, biomedicine and the like.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for extracting and purifying natural collagen from livestock and poultry bones. Background technique [0002] my country is the largest meat producing country in the world. Livestock and poultry bones account for 20%-30% of animal weight, and nearly 20 million tons of livestock and poultry bones can be produced every year. Most of them are processed in primary forms, and the utilization rate is less than 10%. , causing some environmental pollution. At present, the deep processing of bones is gradually being valued by the industry. How to make full use of the bone resources of livestock and poultry is an urgent problem to be solved. [0003] Bone is rich in nutritional value. The crude protein and fat content in bone are very close to those in meat. Phosphoproteins, phospholipids, choline, chondroitin, collagen, etc. in bone marrow cannot be obtained f...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 成晓瑜陈文华裴显庆冯平
Owner CHINA MEAT RES CENT
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