Collagen-silver nanoparticle composite gel, preparation method and application

A technology of silver nanoparticles and collagen, applied in the field of preparation, collagen-silver nanoparticle composite gel, can solve the problems of biological toxicity and safety hazards, cumbersome hydrogel steps, poor mechanical properties of the gel, etc., and achieve low cellularity Effects of toxicity, maintenance of triple helix structure and biological activity, and high mechanical strength

Active Publication Date: 2021-09-07
胶原蛋白(武汉)生物科技有限公司
View PDF18 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] High concentrations of collagen can spontaneously form a gel, but the gel has poor mechanical properties and is easily degraded. Therefore, chemical cross-linking agents are commonly used to cross-link collagen to improve the mechanical properties of collagen gels. However, cross-linking agents The addition of will cause potential biological toxicity safety hazards
Among them, the Chinese patent CN101543643B uses collagen and 3-(methacrylamide) propyl-dimethyl (3-sulfopropyl)amine as raw materials, and uses a chemical cross-linking agent carbodiimide (EDC) to cross-link the collagen to obtain Collagen-based composite corneal substitutes, the residue of chemical reagents such as cross-linking agents in this method will lead to greater toxicity; Chinese patent CN103554527B uses glycosaminoglycans to oxidize to form aldehyde groups that are mixed with collagen, and the aldehydes in glycosaminoglycans The cross-linking reaction between the amino group and the amino group in the collagen is carried out to obtain the collagen hydrogel. The preparation process involves glycosaminoglycan aldydride and hydrazide. The preparation steps of the hydrogel are tedious, the production cycle is long, and the The use of chemical reagents can easily cause cytotoxic reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen-silver nanoparticle composite gel, preparation method and application
  • Collagen-silver nanoparticle composite gel, preparation method and application
  • Collagen-silver nanoparticle composite gel, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation and Characterization of Example 1 Collagen-Silver Nanoparticle Composite Gel

[0042] Collagen solution configuration: Dissolve the solid collagen (type I, II or III) with water or PBS buffer solution, configure it into a collagen solution, and dilute it with water or PBS buffer solution before use.

[0043] AgNO 3 Solution configuration: AgNO 3 The solid is dissolved in water and configured to a concentration of AgNO 3 Solution, diluted with water or PBS buffer solution immediately before use.

[0044] 1. Preparation of type I collagen-silver nanoparticles composite gel

[0045] Taking yak type I collagen as an example, the collagen-silver nanoparticle composite gel was prepared. The specific preparation process was as follows: at room temperature, collagen solution was added to a 12-well plate, and a visible light source (400nm3 Solution, reaction 2hrs. When preparing a sample for rheological performance testing, the composite material is made in a mol...

Embodiment 2

[0055] Example 2 Determination of rheological properties of collagen-silver nanoparticle composite gel

[0056] Prepare the type I collagen-silver nanoparticle composite gel of uniform thickness 1cm * 1cm * 1mm according to Example 1, then place it on the sample stage of the rheometer, fix the scanning frequency of the rheometer, and test the gel Storage modulus G'(Pa) and loss modulus G"(Pa) that vary with strain.

[0057] The results of type I collagen-silver nanoparticles composite gel are as follows: Image 6 As shown, the mechanical properties of the collagen solution without adding Ag ions are poor, only reaching 75Pa. With the increase of the concentration of silver ions, the storage modulus and loss modulus of the type I collagen-silver nanoparticle composite gel are obvious. increased. When the silver ion is 10mg / mL, the storage modulus of the type I collagen-silver nanoparticle composite gel reaches 666Pa; when the silver ion concentration is 25mg / mL, the storage m...

Embodiment 3

[0059] Example 3 Cell Adhesion of Collagen-Silver Nanoparticle Composite Gel

[0060] Take a 24-well plate that has not been treated with TC, put it into slides, add 10 mg / mL BSA solution, 0.01 mg / mL collagen gel, and 1 mg / mL type I collagen to the wells, and turn on the ultra-clean bench for ventilation. Material is allowed to air dry. Add 1 mL of PBS to each well to wash away impurities on the sample surface. Digest the well-growing HFF-1 cells with 1 mL of trypsin, shake them left and right for about 1 minute, and observe the cell shape under a microscope. If the cell shape becomes round and a small amount of cells swim, add 1 mL of FBS to stop the digestion, and use a pipette to stop the digestion. Aspirate the solution, add 2 mL of DMEM solution to blow it down from the bottom of the bottle, and dilute with DMEM to form a cell suspension. Use a hemocytometer to count the number of cells, and add 1 mL of cell suspension in a 96-well plate at 100,000 cells per well in 5% ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Storage modulusaaaaaaaaaa
Storage modulusaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to collagen-silver nanoparticle composite gel, a preparation method and application. The collagen-silver nanoparticle composite gel provided by the invention is obtained by reaction of collagen and a silver ion solution, and the collagen-silver nanoparticle composite gel not only has a complete triple helix structure, but also ensures the original activity of the collagen; the material has relatively high mechanical strength and good antibacterial ability, and has good application potential in clinical medicine; meanwhile, the preparation method of the collagen-silver nanoparticle composite gel is simple, does not involve toxic chemical reagents, and is green and environment-friendly.

Description

[0001] This application claims the priority of the earlier Chinese application with the application date of August 26, 2020, the application number CN202010873027.7, and the title of the invention "a collagen-silver nanoparticle composite gel, preparation method and application", The entire content of this prior application is incorporated in the present application. technical field [0002] The invention belongs to the technical field of biomedical biomaterials, and in particular relates to a collagen-silver nanoparticle composite gel, a preparation method and an application. Background technique [0003] Hydrogels can remain in the lesion for a long time and realize the controlled release of effective molecules, thereby exerting moisturizing, antibacterial, anti-inflammatory and other properties, so they play an important role in the fields of medical dressings and cosmetics. Collagen is widely used in biomedical materials due to its good bioactivity, biocompatibility, bio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08J3/075C08L89/00C08K3/08A61K47/02A61K47/42A61L27/04A61L27/24A61L27/50A61L27/52A61L27/54A61L27/56A61L27/60
CPCC08J3/075A61L27/24A61L27/047A61L27/50A61L27/52A61L27/54A61L27/56A61L27/60A61L27/507A61K47/42A61K47/02C08J2389/00C08K2003/0806C08K2201/011A61L2400/12A61L2300/252A61L2300/404A61L2430/02A61L2430/16A61L2400/04
Inventor 肖建喜付彩虹王莉莉马建瑞田静
Owner 胶原蛋白(武汉)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap