Fluorescent nanometer composite particle, preparation method and application thereof
A technology of composite particles and fluorescent nanometers, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of toxic side effects of living cells, unstable fluorescent signals, limited analytical sensitivity, etc., to improve optical stability and prevent fluorescence leakage , to avoid the effect of bleaching
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Embodiment 1
[0029] (1) Mix 0.0416g 3-aminopropyltrimethoxysilane and 0.0150g (3.85×10 -5 mol) fluorescein isothiocyanate is dissolved in 0.5315g dehydrated alcohol (the molar ratio of 3-aminopropyltrimethoxysilane, fluorescein isothiocyanate and dehydrated alcohol is 6: 1: 300), obtains mixed liquid;
[0030] (2) Under nitrogen atmosphere, carry out coupling reaction 12h in dark condition (the equation of coupling reaction is as figure 1 shown);
[0031] (3) Add 1.95ml of tetraethyl orthosilicate (the density is 0.933g / ml, the amount of substance is 8.73×10 -3 mol), mass percent concentration is 25% ammoniacal liquor 3.85ml and dehydrated alcohol 35ml (the volume ratio of the three is 1: 2: 18), described tetraethyl orthosilicate and step (1) described isothiocyanate fluorescence The molar ratio of the element is 227:1; place it in a light-tight container, stir and react at 15°C for 24 hours to obtain a suspension;
[0032] (4) Centrifuge the suspension obtained in step (3) (rotating ...
Embodiment 2
[0035] (1) Dissolve 0.0416g 3-aminopropyltrimethoxysilane and 0.0150g fluorescein isothiocyanate in 0.5315g absolute ethanol (3-aminopropyltrimethoxysilane, fluorescein isothiocyanate and The molar ratio of absolute ethanol is 6:1:300), obtains mixed solution;
[0036] (2) Under nitrogen atmosphere, carry out coupling reaction 12h in dark condition;
[0037] (3) Add 2.50ml of tetraethyl orthosilicate (the density is 0.933g / ml, the amount of substance is 11.20×10 -3 mol), mass percentage concentration is 25% ammoniacal liquor 4.20ml and dehydrated alcohol 60ml (three volume ratios are 1: 1.68: 24), described tetraethyl orthosilicate and step (1) described isothiocyanate fluorescence The molar ratio of the element is 290:1; place it in a light-tight container, stir and react at 40°C for 24 hours to obtain a suspension;
[0038] (4) Centrifuge the suspension obtained in step (3) (rotating speed: 8000 rm / min), wash twice with absolute ethanol and distilled water respectively, to...
Embodiment 3
[0041] (1) Dissolve 0.0416g 3-aminopropyltrimethoxysilane and 0.0150g fluorescein isothiocyanate in 0.5315g absolute ethanol (3-aminopropyltrimethoxysilane, fluorescein isothiocyanate and The molar ratio of absolute ethanol is 6:1:300), obtains mixed solution;
[0042] (2) Under an argon atmosphere, carry out the coupling reaction in dark conditions for 12 hours;
[0043] (3) Add 2.30ml of tetraethyl orthosilicate (the density is 0.933g / ml, the amount of substance is 10.30×10 -3mol), mass percent concentration is 25% ammoniacal liquor 3.50ml and dehydrated alcohol 48ml (the volume ratio of the three is 1: 1.52: 20.9), described tetraethyl orthosilicate and step (1) described isothiocyanate fluorescence The molar ratio of the element is 267:1; place it in a closed light-proof container, stir and react at 40°C for 24 hours to obtain a suspension;
[0044] (4) Centrifuge the suspension obtained in step (3) (rotating speed: 8000 rm / min), wash twice with absolute ethanol and dist...
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