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Method of extracting scale collagen polypeptide by microwave-assisted enzyme

A fish scale collagen, microwave-assisted technology, applied in the direction of fermentation, can solve the problems of shortening extraction time, low extraction efficiency, and long processing time of fish scale collagen polypeptide, so as to shorten the enzymatic hydrolysis time, improve the degree of hydrolysis, and reduce the production cost. Effect

Inactive Publication Date: 2009-11-25
UNIV OF SHANGHAI FOR SCI & TECH
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Problems solved by technology

[0004] The invention discloses a method for extracting fish scale collagen polypeptides with microwave-assisted enzymes. The purpose is to solve the shortcomings of the current production of fish scale collagen polypeptides, such as long processing time and low extraction efficiency. The microwave-assisted enzyme method of the invention can not only greatly shorten the extraction time Time, and within 60min-120min can reach 70%-90% good degree of hydrolysis, which is conducive to improving production efficiency and large-scale industrial production of fish scale collagen polypeptide

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Embodiment Construction

[0017] Below in conjunction with embodiment the present invention is described in further detail, but present embodiment is not intended to limit the present invention, and every adopt similar method of the present invention and similar variation thereof, all should be included in protection scope of the present invention.

[0018] The method for microwave-assisted enzyme extraction of fish scale collagen polypeptide of the present invention is carried out according to the following steps:

[0019] A) wash the viscous substance and other impurities on the surface of the fish scales, then dry and pulverize the fish scales;

[0020] B) put the fish scales obtained in A) into a 3% hydrochloric acid solution and soak for 24 hours, and change the hydrochloric acid solution once after 12 hours to remove the calcium on the fish scales;

[0021] C) the fish scale obtained in B) is put into saturated sodium carbonate solution and soaked for 24h, wherein the saturated sodium carbonate s...

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Abstract

The invention discloses a method of extracting scale collagen polypeptide by microwave-assisted enzyme, comprising steps of cleaning sticky substance and other impurities on surface of scales, drying and crushing the scales; putting the scales into muriatic acid solution to remove calcium on the scales; putting the scales into alkali solution to remove impure protein on the scales; and drying the treated scales, putting into a microwave synthesizer, adding in enzyme to hydrolyze, wherein weight ratio between the enzyme and the scales is 2:5. Power of the adopted microwave synthesizer is 900w; the microwave time is 90min; and the microwave temperature is 65 DEG C. lastly, filtering the hydrolyzing liquid, freezing and drying the filtrated substance, thus obtaining collagen polypeptide. The method of extracting scale collagen polypeptide shortens enzymolysis time, improves hydrolyzing degree, improves production efficiency and decreases production cost.

Description

technical field [0001] The invention relates to a microwave-assisted enzyme extraction method for fish scale collagen polypeptide. Background technique [0002] Collagen polypeptide is a hydrolyzed product of collagen, and compared with collagen, its molecular weight is relatively low (the average molecular weight is around 20,000). Since Japanese scholars Oshima and others confirmed that gelatin-derived polypeptides can inhibit blood pressure in 1979, successive studies have shown that they have many physiological functions, such as inhibiting blood pressure rise, inhibiting platelet aggregation activity, etc., and these physiological functions are based on collagen. physiologically functional. The difference is that collagen polypeptide (collagen hydrolyzate) overcomes the problems of collagen, gelatin and other polymers with poor solubility and dispersibility, and also improves their digestion and absorption, and can be widely used in food and cosmetics. Therefore, coll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 王欣黄焕刘宝林王俊城宋晨曹晨
Owner UNIV OF SHANGHAI FOR SCI & TECH
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