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Induction method of poplar tetraploid plants

A technology of tetraploid and poplar, applied in the field of plant genetics and breeding

Active Publication Date: 2009-12-09
BEIJING FORESTRY UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of existing poplar tetraploid induction methods, and provide a new induction method for poplar tetraploid plants, which solves the effective treatment period of poplar zygote chromosome doubling It can effectively increase the accuracy and stability of physical and chemical treatment to induce zygotic chromosome doubling, improve the efficiency of tetraploid induction, and save induction reagents and experimental materials.

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  • Induction method of poplar tetraploid plants
  • Induction method of poplar tetraploid plants
  • Induction method of poplar tetraploid plants

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Embodiment 1

[0016] Taking Zheyin No. 3 poplar x Beijing poplar as an example, the infructescences of a certain period after pollination were fixed with FAA (V (70% ethanol): V (acetic acid): V (38% formaldehyde) = 90: 5: 5) , and observed the flocculent fiber development state inside the ovary, and observed the zygote development process through conventional paraffin sections. Due to certain asynchronous development, a large number of zygote development in ovules were counted, and the experimental error disappeared. A total of 213 ovules were counted. It indicated that the occurrence and development of flocculent fibers in the ovary of Poplar Zheyin 3 were closely related to the process of double fertilization and zygote development (Table 1). When the flocculent fibers in the ovary begin to wrap around the base of the ovule ( figure 1 ), or further start to wrap the middle of the ovule but not completely cover the ovule, at this time, most zygotes enter the first mitotic process.

[001...

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Abstract

The invention discloses an induction method of poplar tetraploid plants, belonging to the field of plant inheritance breeding. The method of the invention comprises the following steps: pollination is carried out on female inflorescence of poplar female parent by powder of poplar male parent to form infructescence, when flocculent fiber is formed and wraps ovule in the infructescence ovary, physicochemical treatment is carried out on the infructescence, seed selection is carried out on tetraploid plants; the inventor of the invention discovers by tests that the effective period for carrying out physicochemical treatment to induce the zygote chromosome to be reduplicated is when most zygotes of the poplar is at the first mitosis process and the flocculent fiber starts to wrap but does not completely covet the ovule, thus the induction efficiency of tetraploid plants is improved. The invention avoids verbose links such as fixing, dehydrating, waxing, embedding, slicing, pigmentation, microexamination of the ovule and the like, thus enhancing the timeliness of physicochemical treatment, saving induction reagent and experimental material and improving the precision and stability of the reduplication of zygote chromosome.

Description

technical field [0001] The invention relates to a method for inducing tetraploid plants of plants, in particular to a method for inducing tetraploid plants of poplar trees, and belongs to the field of plant genetics and breeding. Background technique [0002] The most direct and effective way to obtain poplar triploid plants is to use tetraploid plants to cross diploid plants. In some areas of northern Europe and the United States, even the bred tetraploid poplar female plants are directly planted in excellent poplar stands for free pollination, and seeds are collected and seedlings are raised on the tetraploid poplar female plants every year (Xu Weiying. . Harbin: Heilongjiang People's Publishing House, 1988). [0003] Poplar tetraploid breeding can adopt the method of hybridization between triploid and diploid (Nilsson-Ehle H. Production of forest trees with increased chromosome number and increased timber yield. Svensk Papp. Tidn., 1938, 2: 5; Bergstrom I. On the progen...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 王君康向阳鲁敏吴峰
Owner BEIJING FORESTRY UNIVERSITY
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