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Method for immobilizing lipase

A technology of lipase and fabric finishing agent, applied in the direction of being fixed on/in an organic carrier, can solve the problems of weak adsorption, complicated method, weak force, etc., to reduce inactivation phenomenon and simple treatment method , conditions require mild effects

Inactive Publication Date: 2013-08-14
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of inorganic material itself has poor biocompatibility, and the process of immobilization can easily cause changes in protein conformation, and it is easy to introduce new factors that affect the conformation of lipase during its modification, and lipase such as The activity of biomacromolecules is often based on their specific three-dimensional structure, so this kind of porous nanomaterials still needs to be further improved
[0005] In the immobilization method, the common adsorption method is simple to operate but the force is not strong, and the adsorption is not firm; the covalent method has a strong force, but it will lead to the inactivation of some sites of lipase, resulting in a decrease in lipase activity; cross-linking Involves multiple chemical steps making it expensive
[0006] Although there are many existing methods for immobilizing lipase, due to certain shortcomings, some carriers have high costs, some lipases are not firmly combined with carriers, and some methods are complicated, etc., so it is difficult to find a method. Ideal method for immobilizing lipase

Method used

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  • Method for immobilizing lipase
  • Method for immobilizing lipase
  • Method for immobilizing lipase

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Preparation of Immobilized Lipase Using Amino Silicone Oil Polymer AS2-8040 Surface Finishing Carrier

[0030] Fabric finishing agent amino silicone oil polymer AS2-8040, Dow Corning (USA) company.

[0031] Specific steps are as follows:

[0032] 1) Weigh a certain volume of amino silicone oil polymer and dissolve it in n-hexane to prepare a solution with a concentration of 0.5% (v / v);

[0033] 2) According to the bath ratio of silk quality: finishing solution volume = 1:20 (g / ml), soak the woven cloth cut into small pieces in the amino silicone oil solution, vibrate at 120 rpm for 1 hour at 35° C. Dry at ℃ for 30 minutes;

[0034] 3) After modification, the base is arranged and immobilized in a lipase solution at 10°C for 3 hours;

[0035] 4) The immobilized lipase can be dried in a cool and ventilated place.

Embodiment 2

[0036] Example 2 Preparation of Immobilized Lipase Using the Surface Finishing Carrier of Amino Silicone Oil Microemulsion C220A

[0037] Amino silicone oil microemulsion C220A, fabric finishing agent, Academy of Textile Sciences, China.

[0038] Specific steps are as follows:

[0039] 1) According to the fabric cloth quality: the bath ratio of finishing liquid volume=1: 20 (g / ml), the fabric cloth cut into small pieces is soaked in the microemulsion after deionized water dilution, and the consumption of microemulsion C220A is fabric cloth 5% of mass;

[0040] 2) Shake at 35°C and 120rpm for 1 hour, dry the finished fabric at 70°C for 25 minutes, and then bake at 100°C for 1 minute;

[0041] 3) After the modification, the base arrangement was immobilized in a lipase solution at 25°C for 3 hours;

[0042] 4) The immobilized lipase can be freeze-dried.

[0043] The advantages and improvements of the conventional method of the present invention are illustrated by comparison b...

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Abstract

The invention relates to a method for immobilizing lipase. The method comprises that the following steps: using a membrane material such as textile fabric or non-woven fabric and the like as a base fabric carrier; carrying out modified treatment on the surface of the base fabric carrier through a fabric finishing agent to form a hydrophobic surface; soaking the modified base fabric in a lipase solution or paste of lipase; and carrying out drying treatment on the soaked base fabric realize the immobilization of the lipase. The hydrophobic surface of the base fabric after the modified treatmentis favorable for realizing the immobilization of active conformation of the lipase, and effectively reduces the inactivation phenomena of the lipase during immobilizing. The method has the advantagesof high activity of the immobilized lipase, high stability of the immobilized lipase, large selection range of the base fabric, simple and convenient operation, mild condition requirement, easy realization, and the like.

Description

technical field [0001] The invention relates to a method for immobilizing lipase, in particular to a method for immobilizing lipase on a water-insoluble carrier by physical or chemical means to form a reusable immobilized lipase. Background technique [0002] With the deepening of lipase research and the development of lipase engineering, the application of lipase is becoming more and more extensive. The lipase is immobilized on the water-insoluble carrier by physical or chemical methods to form a reusable lipase, which is called immobilized lipase. Immobilized lipase not only maintains the catalytic properties of lipase, but also overcomes the instability of free lipase. It has significant advantages such as repeated or continuous use, and easy separation from reaction products. It is widely used in medicine, light industry, food, etc. industry. [0003] Immobilized lipase improves the stability of industrial lipase and prolongs the service life of lipase, so the immobili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/02
Inventor 谭天伟陈必强程媛媛
Owner BEIJING UNIV OF CHEM TECH