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Method for extracting selenium protein from selenium-enriched green tea

A technology of selenium-enriched green tea and an extraction method, which is applied in the field of extraction of selenium-enriched green tea selenoproteins, can solve the problems of malnutrition of selenium and the like, and achieve the effect of rich content

Inactive Publication Date: 2010-01-20
余芳
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AI Technical Summary

Problems solved by technology

Solve the problem of selenium malnutrition in the population

Method used

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Experimental program
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Embodiment

[0007] The selenium-enriched green tea powder was leached with 40% ethanol at a solid-to-liquid ratio of 1:12 for 10 minutes, and repeated three times to remove tea polyphenols. Suction filtration, discard the filtrate to obtain tea dregs. Then, the treated selenium-enriched green tea dregs were used to extract the selenoproteins in the selenium-enriched tea according to the solid-liquid ratio of 1:30 with 0.1M NaOH solution for 16 hours at a temperature of 40°C. The extraction process is to first carry out cell wall breaking (800W, 180s), and then magnetically stir after extraction, centrifuge (10000rpm, 20min); take the supernatant after centrifugation, slowly add ammonium sulfate to 50% saturation to precipitate selenoproteins, 4 Stand still at ℃ for 12 hours, centrifuge (10000rpm, 20min), dissolve the precipitate with the extract solution, dialyze for 24 hours, and freeze-dry at -40℃ to obtain selenium-enriched protein.

[0008] Beneficial effects achieved:

[0009] 1. S...

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Abstract

The invention provides a method for extracting selenium protein from selenium-enriched green tea, which comprises the following steps: extracting selenium-enriched green tea powder by 40 percent of ethanol for three times repeatedly; removing tea polyphenols to obtain selenium-enriched green tea leaves; extracting the selenium-enriched green tea leaves with high protein and selenium for 16 hours at the temperature of 40 DEG by 0.1 M of NaOH solution, the ratio of liquid to material of the NaOH solution is 1:30, which are the best conditions for extracting the selenium protein. Selenium protein extractive in the selenium-enriched green tea selenium protein extracted by the method at least contains 17 kinds of amino acids, and furthermore the selenium protein extractive contains rich essential amino acids.

Description

Technical field: [0001] The invention provides a method for extracting selenium-enriched green tea selenoprotein. Background technique: [0002] Selenium is one of the essential trace elements for humans and animals. Its important role in cancer prevention, anti-cancer, prevention and treatment of cardiovascular diseases, Keshan disease and Kaschin-Beck disease has been recognized by the world. Selenium can significantly reduce the risk of breast cancer. , skin cancer, colon cancer, liver cancer and other cancers, and has special physiological functions such as relieving heavy metal poisoning. American scientists have proved that selenium deficiency in the body is a common feature of AIDS patients. "Selenium chemical protection" has become the focus of research by many scientists in the world. [0003] The protein content in tea leaves accounts for 15% to 30% of the dry weight of tea, of which 82.05% is gluten, 13.6% is prolamin, 3.47% is albumin, and about 0.87% is globul...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/14
Inventor 余芳
Owner 余芳
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