Method for detecting chemical or biological substance
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A chemical and biochemical technology, applied to the improvement of detection of chemical or biochemical substances, using light scattering probes and chemical detectors to detect chemical or biochemical substances, can solve the application limitations of Raman scattering detectors, pull Weak Mann scattering signal and other problems, to achieve the effect of short test period, easy operation and portability
Active Publication Date: 2010-01-20
北京光生能优科技有限公司
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Conventional Raman scattering detectors are currently limited in application due to technical difficulties
A major limitation in the application of Raman spectroscopy is that the Raman scattering signals obtained from micro-trace amounts of chemicals are very weak
Numerous efforts have been made to enhance the intensity of the Raman scattering signal, however, these effort
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[0057] see Figure 1A , a light scattering probe 100 includes a probe 110 and a detector 105 . The detector 105 includes a nanoscale surface structure. The nanoscale surface structure may include a plurality of nanopillars 108, such as Figure 1BAs shown, multiple nanopores, or other nanometer-sized surface structures. In some embodiments, nanoscale surface structures can be fabricated by coating the surface of the detector 105 with a colloidal suspension solution containing nanoparticles, as described below. The solution can then be evaporated and the nanoparticles deposited onto the surface.
[0058] Taking a nanosurface including nanopillars 108 as an example, a liquid sample can be introduced onto the nanopillars 108 of the detector 105 . The fluid sample may include bodily fluids of a patient or illicit drug user for disease diagnosis or determination of drug use. Examples of bodily fluids may include, but are not limited to, blood, saliva, urine, serum, tears, sweat...
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Abstract
The invention pertains to the field of micro-trace chemical substance detection, relates to a method for detecting chemical or biological substance. The method includes making the sample solution in contact with the nano-scale surface structure in the probe, wherein the nano-scale surface structure includes a plurality of columns on the substrate, a plurality of pores in the substrate, or a plurality of nano-particles in the sample solution; irradiating the sample solution on the nano-scale surface structure of the probe with a laser beam; obtaining the Raman spectrum from the scattered light by a spectrum analyzer; recognizing spectrum signal in the Raman spectrum and whether special chemical or biological substances is included in the sample solution. The sample solution can be sample solution from food, or body fluid from human body. The invention provides a simple and non-invasive detection method, which is easy for operation, applicable in counter-terrorism, criminal investigation, medical diagnosis, disease prevention, industrial process monitoring, environment cleaning and monitoring, counterfeit goods detection, product identification, detection of methanol and ethanol content in alcohol, quality control of food and medicament or the like.
Description
[0001] This application claims priority from US Patent Application No. 12 / 176,383, US Patent Application No. 12 / 246,616 and US Patent Application No. 12 / 403,522. Among them, US 12 / 403,522 patent application is a continuation-in-part (CIP) application of pending US 12 / 176,383 patent application and US 12 / 246,616 patent application. The title of US12 / 246,616 patent application is "system and method applied to food safety detection", and the application date is October 7, 2008; Diagnosing Diseases with Instruments", the filing date was July 20, 2008. U.S. Patent Application No. 12 / 176,383 is part of commonly assigned U.S. Patent Application No. 11 / 681,157 (now issued as U.S. Patent No. 7,428,046), filed March 1, 2007, entitled "Trace Chemical Optical Probes" Continuation of patent application. US Patent Application No. 11 / 681,157 is a continuation of commonly assigned US Patent Application No. 10 / 987,842 (now issued as US Patent No. 7,242,469). US Patent Application No. US10 / 987...
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