In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof

A technology of liquid culture medium and in vitro germination, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., to achieve stable and reliable results and easy observation and measurement

Inactive Publication Date: 2010-01-27
SHANGLUO UNIV
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However, there have been no reports at home and abroad on the in vitr

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  • In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof
  • In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof
  • In-vitro pollen germination liquid medium of balloon flower and method using same to determine pollen viability thereof

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[0027] The present invention provides a method for measuring pollen vigor by in vitro germination culture of Platycodon grandiflorum pollen. On the basis of determining the in vitro germination culture system of Platycodon grandiflorum pollen, in vitro culture of Platycodon grandiflorum pollen is used to determine the germination rate of pollen and the pollen tube Growth status, reliable and effective measurement of platycodon pollen vigor. The present invention will be further described in detail below in conjunction with the accompanying drawings, which is an explanation rather than a limitation to the present invention.

[0028] 1. Collection of Platycodon grandiflorum pollen

[0029] Platycodon grandiflorum pollen was collected from a 2-year-old Platycodon grandiflorum population in the Medicinal Botanical Garden of Shangluo Xiangju Pharmaceutical Company, Shaanxi Province in mid-July 2008. The collection method is: select healthy plants with normal development and no pests and...

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Abstract

The invention discloses an in-vitro pollen germination liquid medium of balloon flower (platycodon grandiflorum (Jacq.) A. DC) and a method using same to determine the pollen viability thereof, particularly a method for reliably and effectively determining the pollen viability of the balloon flower through the in-vitro pollen culture of the balloon flower after the in-vitro pollen germination culture system is determined. The in-vitro pollen germination liquid medium is characterized by using distilled water as the solvent with the pH value being 5.8, wherein, the distilled water comprises the following components: ME3, BK, 100g/L sucrose and 150g/L PEG (polyethylene glycol). The method comprises the following steps: evenly scattering the pollen over the in-vitro pollen germination liquid medium, and culturing at a constant temperature (30 DEG C) under the lighting condition; in-vitro culturing for 1.5h and determining the pollen germination rate and the pollen tube lengths which reflect the pollen viability, particularly, counting the pollen germination rate by the pollen germination standard that the pollen tube length is larger than the pollen particle diameter, and measuring and averaging the pollen tube lengths, wherein, the pollen germination rate reflects the proportion of the viable pollen, and the growth condition of the pollen tube of the in-vitro cultured pollen reflects the physiological condition of the pollen particles.

Description

technical field [0001] The invention belongs to the technical field of measuring plant pollen vigor, and in particular relates to a liquid culture medium for in vitro germination of bellflower pollen, and a method for measuring pollen vigor based on the liquid medium by utilizing the in vitro germination culture of bellflower pollen. Background technique [0002] Campanulaceae (Platycodon grandiflorum (Jacq.) A.DC) belongs to Campanulaceae, Platycodon genus, is a perennial dicotyledonous herbaceous plant, and is a plant with both medicine, food and ornamental functions. The root of Campanulaceae can be used as medicine, and its main active ingredient is saponin, which has a wide range of pharmacological activities such as antitussive, anti-inflammatory, lowering blood pressure, lowering blood sugar, weight loss, anti-tumor, and improving human immunity. Platycodon grandiflora tender seedlings and roots can be eaten. It is a common vegetable in Northeast my country and East A...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12Q1/06G01B5/02
Inventor 刘自刚呼天明杨亚丽薛粉丽刘凤霞
Owner SHANGLUO UNIV
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