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Heartleaf houttuynia herb protease inhibitor and preparation method thereof

A technology for protease inhibition and Houttuynia cordata, which is applied in the field of phytochemistry to achieve the effects of enhancing antioxidant capacity, strong protease inhibitory activity, and reducing lipid peroxidation levels

Inactive Publication Date: 2010-03-10
刘同祥 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the drug extract with protease inhibitor activity isolated from the traditional Chinese medicine Houttuynia cordata has never been reported in the existing literature.

Method used

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  • Heartleaf houttuynia herb protease inhibitor and preparation method thereof
  • Heartleaf houttuynia herb protease inhibitor and preparation method thereof
  • Heartleaf houttuynia herb protease inhibitor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1: Plant Houttuynia cordata extract

[0080] Add 0.5moL·mL to 1g of the dried whole herb of Houttuynia cordata -1 Wet with ammonia water, add 30 times the amount of deionized water, adjust the pH to 8 with 1% sodium hydroxide, boil for 1 hour, filter, concentrate the filtrate under reduced pressure to a relative density of 1.12 at 25°C, and centrifuge to obtain the extract; add 10 times the weight of After suspension in distilled water, the supernatant was extracted with chloroform, and the solvent was recovered to obtain the chloroform extract; the chloroform extract was passed through ion exchange resin, 0.5mol mL -1 Eluted with ammonia water, collected the eluent, concentrated under reduced pressure, and dried in vacuum to obtain Houttuynia cordata extract.

Embodiment 2

[0081] Embodiment 2: the method for preparing plant Houttuynia cordata extract

[0082] Add 0.2moL·mL to 0.5g of the dried whole herb of Houttuynia cordata -1 Wet with ammonia water, add 40 times the amount of deionized water, adjust the pH to 8 with 1% sodium hydroxide, boil for 0.5h, filter, concentrate the filtrate under reduced pressure to a relative density of 1.30 at 20°C, and centrifuge to obtain the extract; add 15 times the weight After suspending in distilled water, the supernatant was extracted with chloroform, and the solvent was recovered to obtain the chloroform extract; -1 Eluted with ammonia water, collected the eluent, concentrated under reduced pressure, and dried in vacuum to obtain Houttuynia cordata extract.

Embodiment 3

[0083] Embodiment 3: the method for preparing plant Houttuynia cordata extract

[0084] Add 1.0moL·mL to 1.5g of the dried whole herb of Houttuynia cordata -1 Wet with ammonia water, add 20 times the amount of deionized water, adjust the pH to 9 with 1% sodium hydroxide, boil for 1.5 hours, filter, concentrate the filtrate under reduced pressure to a relative density of 1.00 at 30°C, and centrifuge to obtain the extract; add 5 times the weight After suspending in distilled water, the supernatant was extracted with chloroform, and the solvent was recovered to obtain the chloroform extract; -1 Eluted with ammonia water, collected the eluent, concentrated under reduced pressure, and dried in vacuum to obtain Houttuynia cordata extract.

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Abstract

The invention discloses a heartleaf houttuynia herb extract with protease inhibiting effects and a preparation method thereof, and a quality control method. In the preparation process, the heartleaf houttuynia herb extract is finally obtained through the steps of decoction, filtration, concentration, extraction and the like. The heartleaf houttuynia herb extract has strong protease inhibition activity, and can reduce acute lung injuries and the lipid peroxidation level of rat lung tissues, increase the oxidation resistance and reduce the release of oxygen free radicals and inflammatory factors; besides, the heartleaf houttuynia herb extract has regulation effect on cell structure and function genes, cell signal transduction related genes, cell metabolism genes, transcription and transcription factor type genes, and protein translation synthesized type related genes of A549 cells of an alveolar epithelial cell source.

Description

technical field [0001] The present invention relates to a drug extract with protease inhibitory effect, in particular to an extract with protease inhibitory activity extracted from the plant Houttuynia cordata; the present invention also relates to a preparation method of the extract and the extract The application in medicine belongs to the field of phytochemistry. Background technique [0002] Houttuynia cordata, also known as Houttuynia cordata thunb, has the effects of clearing heat and detoxification, diuresis, reducing swelling and expulsion of pus, and is an important medicine for treating lung abscess due to phlegm heat, cough and vomiting of pus and blood. Both clinical and animal experiments have shown that the traditional Chinese medicine Houttuynia cordata has certain therapeutic effects on acute lung injury. "Compendium of Materia Medica": "Its leaves have a fishy smell, so it is called Houttuynia cordata." Houttuynia cordata has a fishy and slightly cold tast...

Claims

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Application Information

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IPC IPC(8): A61K36/78A61P11/00G01N21/82G01N30/90
Inventor 刘同祥王继峰牛建昭张宗申
Owner 刘同祥
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