Curcumol hapten and artificial antigen as well as preparation method thereof

Abstract

The invention provides curcumol hapten and artificial antigen as well as a preparation method thereof. The structural formula of the prepared curcumol hapten is shown as a right formula (I), and the structural formula of the artificial antigen is shown as a right formula (II). The preparation method comprises the following steps: taking curcumol as a raw material, and utilizing succinic anhydrideto be reacted with the curcumol to generate curcumol hemisuccinate (Cur-HS) so as to obtain artificial haptens; preparing the curcumol haptens into aqueous pyridine; and then combing the aqueous pyridine and bovine serum albumin to generate the artificial antigen of the curcumol. The invention leads the hapten and the artificial antigen of the curcumol to be synthesized, and a prepared product can be used for studying a sample containing a curcumol composition. The invention has simple synthesis procedure, mild needed experimental conditions, easy availability of the product, higher yield andeffectiveness, and can be carried out at room temperature, be completely used for immunoassay, provide convenience for future study of persons and satisfy the needs to the study at home and abroad.

Description

technical field The invention relates to a curcumol hapten, an artificial antigen and a preparation method thereof, belonging to the technical field of immunology. Background technique Immunoassay is an analysis method based on the specific recognition and binding reaction of antigen-antibody antigen-antibody. It can not only be used for the determination of macromolecular substances such as proteins and enzymes, but also widely used for the determination of small molecular weight drugs, especially for those that are difficult to detect by chromatography. This method has become an indispensable basic detection technology in drug analysis. Immunoassays not only provide the basis for clinical diagnosis, but also can be applied in the research of endocrinology, virology, pharmacology, hematology and oncology, and also in the fields of agricultural science, food science, environmental protection, ecology and forensic science. Progress has also been made. Immunoassay has the ch...

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Problems solved by technology

In particular, it has a unique role in immunoassay detection. However, curcumol is a small molecular natural compound with a small relative molecular mass. It only has reactogenicity but not immunogenicity. Therefore, it cannot be directly detected by immunological methods. Molecular proteins, etc. are coupled to form complexes before they can become complete antigens

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