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Method for targeting and killing prostate tumor cells by oncolytic I type herpes simplex viruses

A technology for herpes simplex and prostate cancer, applied in the field of recombinant viruses, can solve problems such as reducing virus replication efficiency, and achieve the effects of improving oncolytic effect, reducing risk, and improving target specificity

Active Publication Date: 2012-04-25
上海复诺健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antitumor efficacy of many of these oncolytic viruses used to treat patients still needs to be further improved
Oncolytic HSV-1 virus vectors currently used in clinical practice have deleted the gene that can cause neurotoxicity, but doing so will greatly reduce the replication efficiency of the virus

Method used

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  • Method for targeting and killing prostate tumor cells by oncolytic I type herpes simplex viruses
  • Method for targeting and killing prostate tumor cells by oncolytic I type herpes simplex viruses
  • Method for targeting and killing prostate tumor cells by oncolytic I type herpes simplex viruses

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Embodiment Construction

[0022] experimental method

[0023] HSV-1 Nucleic Acid Extraction Take wild-type HSV-1 virus F strain (purchased from Gibco Company and stored in our laboratory, its complete genome sequence and specific gene structure are shown in NCBI, its NCBI serial number is NC_001806) under the condition of MOI=1 Infect cultured Vero cells. After 24-36 hours, when most of the cells showed cytopathic changes (CPE), the cells were collected and transferred to a 15ml centrifuge tube to remove the cell culture medium by centrifugation. Add 0.3ml of cell lysate (including: 100Mm NaCl, 10Mm Tris.HCl pH8.25Mm EDTA pH8 and 0.5% SDS) to lyse the infected cells and treat with RNaseA (10mg / ml) at 65°C for 1 hour. The above lysate was first treated with an equal volume of Phenol was then extracted with an equal volume of phenol / chloroform (1:1) mixture, the DNA-containing aqueous solution was transferred to a new centrifuge tube, and 1 / 2 volume of 7.5M ammonium acetate and 2 volumes of anhydrous al...

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Abstract

The invention relates to a method for targeting and killing prostate tumor cells by oncolytic I type herpes simplex viruses, in particular to a virus treatment method for tumors. The invention also relates to a virus which can be copied in large quantities in tumor cells and has no damage to normal cells. The invention aims to prove that HSV-1 viruses can be copied in specific tissues and tumors through doubly regulating the genetic transcription (prostate specific promoters ARR2PB) and the mRNA translation (5'UTR of rFGF-2) of viral genes ICP27.

Description

technical field [0001] The invention relates to a virus treatment method for tumors. The present invention also relates to a recombinant virus capable of mass replication in tumor cells without damage to normal cells. Background technique [0002] Prostate cancer is the most common non-skin cancer in men and the second leading cause of death in men (Jemal A, Siegel R, Ward E, et al. Cancer statistics, 2008. CA Cancer JClin. 2008; 58(2): 71-96.). In its early stages, localized prostate cancer is often curable with radical prostatectomy and radiation therapy. However, there is basically no cure for locally advanced cancer and metastatic cancer (Martel CL, Gumerlock PH, Meyers FJ, LaraPN. Current strategies in the management of hormone refractory prostate cancer. Cancer Treat Rev. 2003; 29 (3 ): 171-187. Albertsen PC, Hanley JA, Fine J. 20-year outcomes following conservative management of clinically localized prostate cancer. Jama. 2005; 293(17): 2095-2101.). Although horm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01A61K35/76A61P35/00C12R1/93A61K35/763
Inventor 瞿伯荣强东
Owner 上海复诺健生物科技有限公司