Method for preparing feed protein from microbial fermented silkworms

A technology for microbial fermentation and feed protein, which is applied in the field of preparing feed protein by microbial fermentation of silkworm pupae, can solve the problems of unfermented product feed research, chitin-free degradation, etc., so as to enhance the physique of cultured animals, reduce feed costs, and promote animal production. Effect

Inactive Publication Date: 2012-05-23
广东丰信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Xie Jiakun etc. mentioned the application of microbial fermentation technology in silkworm chrysalis deodorization in the patent (patent application number: 96116818) of " silkworm chrysalis microbiological deodorization technology of silkworm chrysalis ", but this patent only relates to the fermentation of a kind of mycelia-producing fungus, and only Research on the odor elimination of silkworm chrysalis has not involved technologies such as compound and mixed fermentation of multiple strains, nor has there been research on the degradation of chitin, nor has there been research on the feed of fermentation products

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  • Method for preparing feed protein from microbial fermented silkworms
  • Method for preparing feed protein from microbial fermented silkworms
  • Method for preparing feed protein from microbial fermented silkworms

Examples

Experimental program
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Effect test

Embodiment 1

[0031] a. Medium preparation: LB liquid medium (adjusting pH to 7.0) consisting of 5 grams of peptone, 5 grams of yeast extract, 3 grams of sodium chloride and 1 liter of water is poured into a triangular flask to prepare Bacillus subtilis seed culture Base and liquid expansion medium; consisting of 2 grams of peptone, 5 grams of NaCl, 2 grams of glucose, 3 grams of KH 2 PO 4 , 3 grams K 2 HPO 4 , 0.2 g MgCl 2 , 0.05 g CaSO 4 , 2 grams of starch and 1 liter of water (adjusting the pH to 6.5) to form a medium, pour it into a triangular flask to make a lactic acid bacteria seed medium and a liquid expansion medium; The wort culture medium that liter of water (adjusting pH is 6.0) is poured in the triangular flask, makes yeast seed culture medium and liquid expansion medium; 30 grams of sucrose, KNO 3 2 g, MgSO 4 0.5 g, KCl 0.5 g, K 2 HPO 4 1 g, FeSO 4 ·7H 2 O0.01 gram adds the culture medium that 1 liter of water forms, pours in the triangular flask, makes Aspergillus ...

Embodiment 2

[0035] The preparation of culture medium and bacterial solution were the same as in Example 1.

[0036] c. Bacillus subtilis, Aspergillus oryzae, Aspergillus sojae seed liquid are mixed uniformly by weight 3: 1: 1, as silkworm chrysalis powder fermented bacterium liquid, be the inoculum amount inoculation of 8% of silkworm chrysalis powder gross weight with weight In the silkworm chrysalis powder, add 3% molasses and 0.05% K of the total weight of the silkworm chrysalis powder simultaneously 2 HPO 4 , mixed evenly (the initial pH value is 7.0), and fermented at 32°C for 30h. Then brewer's yeast and lactic acid bacteria seed liquid are mixed in a weight ratio of 1:1, as the fermented bacterium liquid of silkworm chrysalis powder, and inoculated in the fermented material with an inoculation amount of 3% of the total weight of silkworm chrysalis powder by weight, and the material is stirred evenly After that, it was fermented at 35°C for 24 hours, and then the fermented silkwor...

Embodiment 3

[0038] The preparation of culture medium and bacterial solution were the same as in Example 1.

[0039] c. Bacillus subtilis, Aspergillus oryzae, Aspergillus sojae seed liquid are mixed uniformly by weight 2: 1: 1, as silkworm chrysalis powder fermented bacterium liquid, be the inoculum amount inoculation of 5% of the total weight of silkworm chrysalis powder with weight In the silkworm chrysalis powder, add 3% molasses and 0.08% K of the total weight of the silkworm chrysalis powder simultaneously 2 HPO 4 , mixed evenly (the initial pH value was 7.0), and fermented at 30° C. for 36 hours. Then brewer's yeast and lactic acid bacteria seed liquid are mixed in a weight ratio of 1:2, as the fermented bacterium liquid of silkworm chrysalis powder, and inoculated in the fermented material with an inoculation amount of 2% of the total weight of silkworm chrysalis powder by weight, and the material is stirred evenly Afterwards, ferment at 33°C for 18 hours, and then dry the ferment...

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Abstract

The invention discloses a method for preparing feed protein from microbial fermented silkworms, which comprises the following steps: inoculating microbes into silkworm powder; adding a carbon source and inorganic salt; fermenting and culturing for 24 to 72 hours at a temperature between 28 and 35 DEG C; and drying to obtain the feed protein. The method improves the palatability and the nutritional value of silkworm protein in the feed application. The produced silkworm protein feed product has good quality without odor of silkworms, has sour and delicious taste without organic solvent residues, contains protein easy to digest, has low production cost and simple and convenient production process, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for preparing feed protein, in particular to a method for preparing feed protein by using microorganisms to ferment silkworm chrysalis. Background technique [0002] Silkworm chrysalis is a by-product of the silk reeling industry, and will be widely and deeply developed in various fields such as agriculture, industry, food, and medicine. my country is a big country for raising silkworms, and the output of silkworm cocoons accounts for 70% of the total cocoons in the world. Among them, the output of fresh silkworm chrysalis can reach 500,000 tons, and the output of dry silkworm chrysalis can reach 120,000 tons. It is a valuable natural resource with considerable development and utilization value. , of which the annual output of Guangdong and Guangxi is 50,000 tons. Silkworm chrysalis is a protein feed resource with high feeding value. The protein in silkworm chrysalis accounts for 66.85%, and the amino acid content is: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/16A23K10/12A23K20/147A23K40/00
Inventor 赵祥杰邝哲师叶明强罗国庆肖更生廖森泰
Owner 广东丰信生物科技有限公司
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