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Culture medium for tissue culture of asexual propagation of butterfly orchid

A technique of asexual reproduction and tissue culture, applied to plant cells, etc., to achieve the effects of inhibiting pollution, facilitating survival, and reducing the concentration of toxins

Active Publication Date: 2011-08-31
浙江传化生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Culture medium for tissue culture of asexual propagation of butterfly orchid

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Experimental program
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Effect test

Embodiment 1

[0030] Phalaenopsis asexual propagation tissue culture medium composition is as follows:

[0031] NH 4 NO 3 : 1650mg / L KNO 3 : 1900mg / L

[0032] CaCl 2 ·H 2 O: 440mg / L MgSO 4 ·7H 2 O: 370mg / L

[0033] K H 2 PO 4 : 170mg / L KI: 0.83mg / L

[0034] h 3 BO 3 : 6.2mg / L MnSO 4 4H 2 O: 22.3mg / L

[0035] ZnSO 4 ·7H 2 O: 8.6 mg / L Na 2 MoO 4 ·5H 2 O: 0.25mg / L

[0036] CuSO 4 ·5H 2 O: 0.025mg / L CoCl 2 ·6H 2 O: 0.025mg / L

[0037] FeSO 4 ·H 2 O: 27.8 mg / L Na 2 EDTA·2H 2 O: 37.3mg / L

[0038] Inositol: 100.0mg / L Niacin: 0.5mg / L

[0039] Pyridoxine hydrochloride: 0.5mg / L Thiamine hydrochloride: 0.1mg / L

[0040] Glycine: 2.0mg / L

[0041] On the basis of the above MS medium, it also added: starch grafted acrylonitrile superabsorbent resin: 0.5g / L and nano Ag 2 O: 1mg / L.

[0042] The auxiliary agents are as follows: sucrose: 3%; agar: 6.5g / L; banana juice: 150g / L; 6-benzylpurine: 3mg / L; activated carbon: 0.5g / L.

Embodiment 2

[0044] Phalaenopsis asexual propagation tissue culture medium composition is as follows:

[0045] NH 4 NO 3 : 1650mg / L KNO 3 : 1900mg / L

[0046] CaCl 2 ·H 2 O: 440mg / L MgSO 4 ·7H 2 O: 370mg / L

[0047] K H 2 PO 4 : 170mg / L KI: 0.83mg / L

[0048] h 3 BO 3 : 6.2mg / L MnSO 4 4H 2 O: 22.3mg / L

[0049] ZnSO 4 ·7H 2 O: 8.6 mg / L Na 2 MoO 4 ·5H 2 O: 0.25mg / L

[0050] CuSO 4 ·5H 2O: 0.025mg / L CoCl 2 ·6H 2 O: 0.025mg / L

[0051] FeSO 4 ·H 2 O: 27.8 mg / L Na 2 EDTA·2H 2 O: 37.3mg / L

[0052] Inositol: 100.0mg / L Niacin: 0.5mg / L

[0053] Pyridoxine hydrochloride: 0.5mg / L Thiamine hydrochloride: 0.1mg / L

[0054] Glycine: 2.0mg / L

[0055] Also added on the basis of the above MS medium: sodium polyacrylate: 1.0g / L and nano Ag 2 O: 1mg / L.

[0056] The auxiliary agents are as follows: sucrose: 3%; agar: 6.0g / L; banana juice: 150g / L; 6-benzylpurine: 3mg / L; activated carbon: 0.5g / L.

Embodiment 3

[0058] Phalaenopsis asexual propagation tissue culture medium composition is as follows:

[0059] NH 4 NO 3 : 1650mg / L KNO 3 : 1900mg / L

[0060] CaCl 2 ·H 2 O: 440mg / L MgSO 4 ·7H 2 O: 370mg / L

[0061] K H 2 PO 4 : 170mg / L KI: 0.83mg / L

[0062] h 3 BO 3 : 6.2mg / L MnSO 4 4H 2 O: 22.3mg / L

[0063] ZnSO 4 ·7H 2 O: 8.6 mg / L Na 2 MoO 4 ·5H 2 O: 0.25mg / L

[0064] CuSO 4 ·5H 2 O: 0.025mg / L CoCl 2 ·6H 2 O: 0.025mg / L

[0065] FeSO 4 ·H 2 O: 27.8 mg / L Na 2 EDTA·2H 2 O: 37.3mg / L

[0066] Inositol: 100.0mg / L Niacin: 0.5mg / L

[0067] Pyridoxine hydrochloride: 0.5mg / L Thiamine hydrochloride: 0.1mg / L

[0068] Glycine: 2.0mg / L

[0069] On the basis of the above MS medium, it also added: polyacrylamide: 0.8g / L and nano Ag 2 O: 1mg / L.

[0070] The auxiliary agents are as follows: sucrose: 3%; agar: 6.2g / L; banana juice: 150g / L; 6-benzylpurine: 3mg / L; activated carbon: 0.5g / L.

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Abstract

The invention discloses a culture medium for tissue culture of asexual propagation of butterfly orchid. A super absorbent resin at a concentration of between 0.01 and 1g / L and an antimicrobial, namely nano-Ag2O at a concentration of between 1 and 5mg / L are added based on an MS culture medium; and sucrose, agar, banana juice, and a cytokinin, namely 6-benzylpurine are also added. The culture medium adopts the super absorbent resin to partially substitute the agar, which can ensure that the culture medium achieves the proper coagulability, has proper support effect on a culture, can also ensurethat nutrient substances are easy to diffuse in the absorbent resin, and improve the absorption efficiency of nutrients; metabolites generated by the culture during the culture are easy to permeate and diffuse through the super absorbent resin, and the concentration of toxin on the contact surface of the culture is reduced; and added nano-Ag2O particles help to decompose the metabolites of the culture, and effectively inhibit the pollution caused by endophytes of explants.

Description

technical field [0001] The invention relates to a tissue culture medium, in particular to a phalaenopsis asexual reproduction tissue culture medium. Background technique [0002] Phalaenopsis is one of the most widely cultivated species of Cattleya in the world. It has the reputation of "Queen of Cattleya". Phalaenopsis has large flowers, long flowering period, bright colors and endless changes. It is the most popular ornamental Cattleya species. [0003] Phalaenopsis is relatively single-plant, and ramets are rarely produced during cultivation. Tissue culture vegetative propagation is one of methods commonly used in current Phalaenopsis industrial production at present, namely so-called clonal propagation, which has the advantages of fast propagation, large quantity and pure seedling varieties. It can be said that the Phalaenopsis sold in today's flower market are basically cultivated with tissue culture seedlings propagated by tissue culture. The phalaenopsis peduncle si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 倪惠珠荣松王猛
Owner 浙江传化生物技术有限公司