Technology for producing alpha-cyclodextrins by biological method

A production process and cyclodextrin technology are applied in the production process field of biological production of α-cyclodextrin, which can solve the problem of lack of α-cyclodextrin production technology, low solubility of β-cyclodextrin, lack of excellent strains, etc. The problem is that the removal process is simple, the production cost is low, and the impurities are less.

Active Publication Date: 2012-10-10
山东黄三角生物技术产业研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the three commonly used cyclodextrins, β-cyclodextrin has the smallest water solubility and is easy to obtain by fractional crystallization. Therefore, β-cyclodextrin is widely prepared and widely used in industrial production, but β-cyclodextrin Due to the low solubility of cyclodextrin, its application in drug inclusion is limited. At present, scientific and technological workers are working on improving the solubility of β-cyclodextrin, and have developed a series of high-solubility β-cyclodextrin Derivatives (Yuan Chao, Jin Zhengyu, Wang Chenguang. Modified cyclodextrin and its application, Grain and Oil, 2006, (05))
At present, the main problems of biological production of α-cyclodextrin are: one, the lack of good strains, the lack of high yield and directional production of α-cyclodextrin cyclodextrin glucosyltransferase (CGTase) on the market; two, the lack of α-cyclodextrin The corresponding production process of cyclodextrin, in view of the high solubility of α-cyclodextrin in water, it is necessary to develop a set of efficient and economical separation and extraction methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Raw material pretreatment:

[0029] The potato starch slurry is adjusted according to the concentration of 10%, and stirred for 5-15 minutes under the condition of 50-90 DEG C, so that the starch granules are fully swollen.

[0030] Enzymatic production process:

[0031] After pretreatment, set the temperature to 30°C, adjust the pH to 5.0, add α-CGTase at a rate of 10 units per gram of starch, and then add 5% decanyl alcohol by reaction volume, and fully react for 8-10 hours.

[0032] α-CGT extraction process:

[0033]Filter the reaction solution directly, wash the filter cake with distilled water 2-3 times, collect the filter cake, including the complex precipitate formed by n-decyl alcohol and α-CGT and unreacted starch; redissolve the filter cake, and then use Steam distillation to remove n-decyl alcohol, after the distillation is complete, filter the distillate to remove unreacted starch to obtain an aqueous solution of α-CGT; evaporate and concentrate the aqueou...

Embodiment 2

[0038] Raw material pretreatment:

[0039] Same as Example 1

[0040] Enzymatic production process:

[0041] After pretreatment, set the temperature to 40°C, adjust the pH to 5.0, add α-CGTase at a rate of 10 units per gram of starch, and then add 5% decanyl alcohol by reaction volume, and fully react for 8-10 hours.

[0042] α-CGT extraction process:

[0043] Same as Example 1

[0044] The results are shown in Table 1. The total conversion rate of starch is as high as 65%, the proportion of α-CGT in the product is 75%, and the proportion of β-CGT is 25%.

Embodiment 3

[0046] Raw material pretreatment:

[0047] Same as Example 1

[0048] Enzymatic production process:

[0049] After pretreatment, set the temperature to 50°C, adjust the pH to 5.0, add α-CGTase at a rate of 10 units per gram of starch, and then add 5% decanyl alcohol to react fully for 8-10 hours.

[0050] α-CGT extraction process:

[0051] Same as Example 1

[0052] The results are shown in Table 1. The total conversion rate of starch is as high as 63%, the proportion of α-CGT in the product is 72%, and the proportion of β-CGT is 28%.

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Abstract

The invention discloses technology for producing alpha-cyclodextrins (alpha-CGT) by a biological method, belonging to the technical field of cyclodextrins production. The alpha-CGT is produced by adopting alpha-cyclodextrins glycosyltransferase (alpha-CGTase) derived from paenibacillus macerans (China Center for Type Culture Collection of Microbe, CCTCCM 208063). By applying the technology to produce alpha-CGT, the total conversion of starch is as high as 71%, and in the product, alpha-CGT accounts for 80% and beta-CGT accounts for 20%. The technology has the advantages of simple flow, short production cycle, high conversion and high purity of the obtained alpha-CGT, etc.

Description

technical field [0001] The invention relates to a production process for producing α-cyclodextrin by a biological method, and belongs to the technical field of cyclodextrin production. Background technique [0002] Cyclodextrins (Cyclodextrins, usually abbreviated as CD) are a class of D-glucopyranose units connected end-to-end by alpha-1,4-glycosidic bonds generated by starch or polysaccharides under the action of cyclodextrin glucosyltransferase. The general term for cyclic compounds, common molecules with 6, 7 and 8 glucose units, are called α-, β- and γ-cyclodextrin, respectively. Because cyclodextrin can form clathrates with many guest molecules, thereby changing the physical and chemical properties of the guest molecules such as solubility, stability, etc., it is widely used in food, medicine, agriculture, textiles, environmental protection, cosmetics, biotechnology and analytical chemistry, etc. field has a wide range of applications. Among the three commonly used c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/18C12N9/10C12R1/01
Inventor 吴敬吴丹王宁陈坚陈晟
Owner 山东黄三角生物技术产业研究院有限公司
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