Laser-induced fluorescence analyzer with PCR-CE coupled microfluidic chip

Abstract

The invention discloses a laser-induced fluorescence analyzer with a PCR-CE coupled microfluidic chip, relating to a biomedical detection analyzer. In the structure of the laser-induced fluorescence analyzer, a machine shell (00) is internally provided with a PCR unit (10), a CE unit (20), a laser-induced fluorescence detection unit (30) and a signal collecting and processing unit (40); after mutually connecting with t, the PCR unit (10) and the CE unit (20) are connected with each other firstly and then connected with the laser-induced fluorescence detection unit (30); and the laser-induced fluorescence detection unit (30) is connected with the signal collecting and processing unit (40). The invention integrates PCR, electrophoretic separation, and laser-induced fluorescence detection into a whole, has the advantages of small size, simple structure, easy operation, low cost, stable performance, high analysis and detection sensitivity, and short disease diagnosis period, and is suitable for disease diagnosis of genes related to diseases.

Description

technical field [0001] The invention relates to a biomedical detection analyzer, in particular to a PCR-CE (polymerase chain reaction-capillary electrophoresis) combined microfluidic chip laser-induced fluorescence analyzer. Background technique [0002] The micro-total analysis unit is a brand-new field that began to rise in the early 1990s. The micro-total analysis unit involves many disciplines, such as analytical chemistry, physics, biology, medicine, pharmacy, micro-processing technology, electronics, computers, etc., through the integration and miniaturization of sample processing, separation and detection The basic functions of routine laboratories, so as to achieve the goals of low cost, high throughput, intelligence and portability. [0003] Lab-on-a-chip technology integrates the functions of the entire laboratory, from sample addition, reaction, separation, detection and other processes, on a microchip of only a few square centimeters. It has been used in chemica...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the problems of long time for processing samples, large amount of samples, large manual workload, high cost and cycle time existing in the whole process of existing disease gene diagnosis sample extraction, PCR sample amplification, electrophoresis separation, and laser-induced fluorescence detection. Long and other problems, providing a PCR-CE combined microfluidic chip laser-induced fluorescence analyzer

Images