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Culture medium formula of edible fungus second class fungus (protospecies) and making method

The technology of a medium formula and a production method is applied in the field of medium formulation and production of secondary species (original species) of edible fungi, and can solve the problems of slow germination of bacteria, cumbersome production process and high pollution rate, and achieve fast germination of mycelium. , The production process is simple, and the quality of the bacteria is good.

Inactive Publication Date: 2010-06-09
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although grain strains have the characteristics of long storage time and rapid germination, the production process of the strains is cumbersome, and the pollution rate is high during the germination period; the original strains made of wood chips, cotton seed hulls, etc. When used for inoculation, it needs to be used in blocks, otherwise the mycelium of the strain will break and the germination of the strain will be slow, which will directly affect the quality of the tertiary species

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0012] Specific embodiment one: flat mushroom, shiitake mushroom secondary species (original species) are made

[0013] The production process of the strain is: making ceramsite soaking solution → soaking ceramsite → preparing culture material according to the formula ratio → bottling → sterilization → inoculation → germ management.

[0014] (1) Production of ceramsite soaking solution

[0015] The soaking solution formula is:

[0016] a. Potato 200g, glucose 20g, water 1000ml;

[0017] b. Potatoes 100-200g, sawdust 100-150g, glucose 20g, KH 2 PO 4 0.5~1g, MgSO 4 ·7H 2 O 0.75g, V B1 5~10mg, water 1000m1;

[0018] According to the volume of the production liquid, accurately weigh the processed potatoes according to the formula (peeled, washed, and those with sprouts must be dug out), cut into thin slices, and other raw materials. Add an appropriate amount of water into the steamer, put potatoes, etc., heat to boil, and cook until the potato chips are crispy but not ro...

specific Embodiment 2

[0029] Specific embodiment two: Bailing mushroom, Pleurotus eryngii secondary species (original species) are made

[0030] The production process of the strain is: making ceramsite soaking solution → soaking ceramsite → preparing culture material according to the formula ratio → bottling → sterilization → inoculation → germ management.

[0031] (1) Production of ceramsite soaking solution

[0032] The soaking solution formula is:

[0033] a. Potato 200g, glucose 20g, water 1000ml;

[0034] b. Potato 200g, soybean powder 20g, glucose 20g, maltose 15g, V B1 5~10mg, water 1000ml;

[0035] According to the volume of the production liquid, accurately weigh the processed potatoes according to the formula (peeled, washed, and those with sprouts must be dug out), cut into thin slices, and other raw materials. Add an appropriate amount of water into the steamer, put potatoes, etc., heat to boil, and cook until the potato chips are crispy but not rotten. Use a glass rod to gently p...

specific Embodiment 3

[0046]Specific embodiment three: Ganoderma lucidum, Flammulina velutipes secondary species (original species) are made

[0047] The production process of the strain is: making ceramsite soaking solution → soaking ceramsite → preparing culture material according to the formula ratio → bottling → sterilization → inoculation → germ management.

[0048] (1) Production of ceramsite soaking solution

[0049] The soaking solution formula is:

[0050] a. Potato 200g, glucose 20g, water 1000ml;

[0051] b. Potatoes 150-200g, sawdust 50-100g, bran 50g, glucose 20g, KH 2 PO 4 1~1.5g, MgSO 4 ·7H 2 O0.5~1g, V B1 5~10mg, water 1000ml;

[0052] According to the volume of the production liquid, accurately weigh the processed potatoes according to the formula (peeled, washed, and those with sprouts must be dug out), cut into thin slices, and other raw materials. Add an appropriate amount of water into the steamer, put potatoes, etc., heat to boil, and cook until the potato chips are ...

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Abstract

The invention relates to a culture medium formula of an edible fungus second class fungus (protospecies) and a making method. The making method takes ceramic granules as a main raw material to make the culture medium of the edible fungus second class fungus (protospecies) and comprises the following process flow of: making ceramic granule soaking solution; soaking the ceramic granules; preparing cultivation composts in proportion according to the formula; bottling; sterilizing; carrying out aseptic inoculation; carrying out burgeon management; carrying out strain examination; and using or storing the culture medium temporarily. The making method has the advantages of quick strain making, better strain quality, grain saving, high efficiency, and the like.

Description

technical field [0001] The present invention relates to the invention of a second-grade species (original species) medium formula and a preparation method in the production process of edible fungi. Background technique [0002] The strains used in the actual production of edible fungi are mycelium cultures that are graded for reproduction. According to the "National Measures for the Interim Management of Edible Fungus Strains", the strains are divided into primary, secondary, and tertiary. However, in production practice, the strains are generally divided into mother species (first-class species), original species (second-class species) and production species (tertiary species). The production of edible fungus strains is completed in the order of primary strains, secondary strains, and tertiary strains. The first-class strains are generally pure cultures obtained by spore separation or tissue separation, and transplanted on a test tube slant culture medium. The first-clas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 刘连强张志军訾惠君周永斌魏雪生王玫王文志
Owner 天津市农业科学院
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