Organic phosphorus degrading enzyme preparation and preparation method thereof

An organophosphorus and enzyme-degrading technology, applied in the field of microorganisms, can solve problems such as toxicity and achieve better results

Inactive Publication Date: 2012-07-25
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, some organophosphates are highly toxic to bees, fish

Method used

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  • Organic phosphorus degrading enzyme preparation and preparation method thereof
  • Organic phosphorus degrading enzyme preparation and preparation method thereof
  • Organic phosphorus degrading enzyme preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Fermentation of seed liquid: Beef extract peptone medium was sterilized and inoculated with Methylobacterium at an inoculum size of 10% by volume, and cultured at 30° C. and 180 r / min for 60 h.

[0014] 2) Fermentation tank fermentation: move the seed liquid into the seed tank according to the inoculation amount of 8% by volume, and carry out fermentation and cultivation. The speed is 300 rpm, and the fermentation time is 60 hours.

[0015] 3) Centrifuge the fermentation broth at 4°C, 7000r / min. for 30min, discard the supernatant, and add 1 / 40 of the fermentation broth volume pH7.5, 0.05mol / L of EDTA and 1 / 15 of the fermentation broth volume to the bacteria sludge Gently shake the sucrose solution to make the bacteria fully suspend, put it in ice bath for 60min, centrifuge at 7000r / min for 30min at 4°C, discard the hypertonic solution, add 1 / 40 of the volume of the fermentation broth to the sludge, and gently Shake well, put in an ice bath for 60 minutes, centrifuge...

Embodiment 2

[0023] The difference from Example 1 is:

[0024] 1) Seed liquid fermentation: Beef extract peptone medium was sterilized and inoculated with Methylobacterium at an inoculum size of 5% by volume, cultured at 35° C. and 180 r / min for 48 hours.

[0025] 2) Fermentation tank fermentation: move the seed liquid into the seed tank according to the inoculation amount of 5% by volume, and carry out fermentation and cultivation. The conditions are: tank temperature 25°C, tank pressure 0.02MPa, ventilation rate 1:1.0v / v / min, stirring The speed is 300 rpm, and the fermentation time is 48 hours.

[0026] 3) Centrifuge the fermentation broth at 4°C, 6000r / min for 40min. Discard the supernatant, add 1 / 30 of the fermentation broth volume pH7.5, 0.05mol / L of EDTA and 1 / 10 of the fermentation broth volume to the sludge Gently shake the sucrose solution to make the bacteria fully suspended, ice bath for 30min, centrifuge at 4°C, 6000r / min for 40min, discard the hypertonic solution, add 1 / 30 of...

Embodiment 3

[0034] The difference from Example 1 is:

[0035] 1) Fermentation of seed liquid: Beef extract peptone medium was sterilized and inoculated with Methylobacterium at an inoculum size of 5% by volume, cultured at 32° C. and 180 r / min for 52 hours.

[0036] 2) Fermentation tank fermentation: transfer the seed liquid into the seed tank according to the inoculation amount of 5% by volume, and carry out fermentation and cultivation. The speed is 300 rpm, and the fermentation time is 52 hours.

[0037] 3) Centrifuge the fermentation broth at 4°C, 8000r / min for 35min. Discard the supernatant, add 1 / 30 of the fermentation broth volume pH7.5, 0.05mol / L of EDTA and 1 / 10 of the fermentation broth volume to the sludge Gently shake the sucrose solution to make the bacteria fully suspended, ice bath for 50min, centrifuge at 8000r / min for 35min at 4°C, discard the hypertonic solution, add 1 / 30 of the fermentation liquid volume of ice water to the bacterial sludge, and gently Shake well, put...

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Abstract

The invention relates to microbe technology, in particular to a degrading enzyme preparation for degrading residual organic phosphorus on vegetables and fruits and a preparation method thereof. The invention particularly relates to organic phosphorus degrading enzyme preparation obtained by fermenting Methylobacterium. The preparation process comprises the following steps: after fermenting the inoculated strain, centrifugally collecting thalli, and breaking thalli to obtain the organic phosphorus degrading enzyme preparation. The organic phosphorus pesticide degrading enzyme preparation can degrade residual organic phosphorus pesticides on vegetables and fruits.

Description

technical field [0001] The invention relates to microbial technology, in particular to an enzyme preparation for degrading residual organophosphorus on vegetables and fruits and a preparation method thereof. Background technique [0002] Organophosphorus pesticides are the main category of pesticides and play an important role in agricultural production. However, the problems of killing non-target organisms and polluting the environment cannot be ignored. Low doses of organophosphorus residues in vegetables, fruits and other foods can cause chronic toxicity to humans and induce polyneuropathy and stroke. In addition, some organophosphates are highly toxic to bees and fish. The residual toxicity of organophosphorus pesticides has attracted more and more attention. The degradation of organic phosphorus in the environment mainly includes three forms: photochemical degradation, chemical degradation, and microbial degradation. At present, the use of microorganisms and their m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02A62D101/04
Inventor 张惠文蔡颖慧苏振成李旭
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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