Device and method for arranging a plurality of cells at same plane and controlling cells

Abstract

The invention discloses a device and a method for arranging a plurality of cells at the same plane and controlling the cells, comprising: a thin film with micro-hole arrays which is located at the upper surface of a base; a PDMS stamp whose lower surface is provided with a micro-groove unit and is coated on the thin film; the micro-groove unit comprises: an intermediate groove and a left-side groove and a right-side groove which are located at the left side and the right side of the intermediate groove; the middle sections of the left-side groove and the right-side groove are parallel to the intermediate groove; the both end sections except for the middle sections of the left-side groove and the right-side groove slant towards the direction away from the intermediate groove; the end of the groove is provided with a vertical through hole which is communicated with the corresponding groove; the holes of the thin film are all located in the micro-groove of the stamp; and a micro-pipeline is provided with different cell suspensions, the cell grows in the micro-pipeline and adheres to the micro-pipeline; the stamp is removed to realize the co-culture of a plurality of cells at the same plane; and then the thin film is removed to realize the free movement of the plurality of co-cultured cells at the same plane; the method is simple to operate, the used material is cheap, and the invention can be used for high-throughput screening of useful drugs for cells.

Description

technical field

[0001] The invention belongs to the device and method for arranging multiple cells on the same plane and manipulating them, belonging to the field of biotechnology. Background technique

[0002] Accurately arranging a variety of cells on the same plane not only plays a wide role in basic biology, such as the study of cell migration and cell interaction, but also provides a platform for tissue engineering and drug screening. Scientists have developed some related methods:

[0003] For example, Document 1: Chui, D.T.et al.Proc.Natl.Acad.Sci.U.S.A.2000, 97, (6), 2408-2413, the Whitesides group of Harvard University, using a PDMS microfluidic channel with a three-dimensional structure, on the same surface Array of various cells. This method first makes a PDMS "stamp" with a three-dimensional structure. After the "stamp" is combined with the substrate for culturing cells, different kinds of cells are introduced into the microfluidic channel, so that the cells wi...

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