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Novel detection method of nanogold labeling-aflatoxin B1

A technology of aflatoxin and detection method, which is applied in the field of AFB1 new ultra-sensitive detection system, can solve the problems of low limit standard and exacerbation

Inactive Publication Date: 2010-06-23
JIANGNAN UNIV
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Problems solved by technology

However, with the improvement of people's living standards, the hazard factors related to food safety (such as AFB 1 ) limit standards will be lower and lower, and at the same time, the intensification of international food trade barriers requires the development of more sensitive and rapid detection methods as soon as possible

Method used

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  • Novel detection method of nanogold labeling-aflatoxin B1
  • Novel detection method of nanogold labeling-aflatoxin B1
  • Novel detection method of nanogold labeling-aflatoxin B1

Examples

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Effect test

example 1

[0057] AFB in the edible peanut oil of example 1 1 detection

[0058] 1 material

[0059] 1.1 Instrument

[0060] Multiskan MK3 microplate reader (Thermo Company, USA) was used to measure the absorbance after silver enhancement; MPI-B multi-parameter chemiluminescence analysis system (Xi'an Ruimai Analytical Instrument Co., Ltd.) was used for dissolution chemiluminescence determination; DF-101S heat collection A magnetic heating stirrer (Yuhua Instrument Factory, Gongyi City, Henan Province) was used for the synthesis of gold nanoparticles; a TU-1900 UV-Vis spectrophotometer (Beijing Puyi General Instrument Co., Ltd.) was used for the determination of nano-absorption spectra; a TecnaiG220 transmission electron microscope (TEM ) (US FEI Company) for the morphological characterization of the synthesized gold nanoparticles. LH586-2 constant temperature water bath (Shanghai Jingke Industrial Co., Ltd.) is used for reaction temperature control. 5804R high-speed centrifuge (Shan...

example 2

[0091] Example 2 Chemiluminescence and enzyme-linked immunoassay (ELISA) in AFB 1 Result correlation in detection

[0092] 1 With reference to the chemiluminescence method in Example 1, different concentrations of standard AFB added in edible peanut oil were detected 1 At the same time, the ELISA method was carried out, and the correlation analysis was carried out between the two results.

[0093] AFB for control experiments 1 The enzyme-linked immunosorbent assay kit was purchased from Wuxi Institute of Microbiology Co., Ltd., and the kit consisted of a coated antibody reaction plate (48 wells); A reagent: sample diluent (1 bottle); B reagent: AFB 1 Standard solution [1 set (0, 0.1, 0.25, 0.5, 1, 2ng / mL)]; C reagent: enzyme-labeled antigen (2 / 1 / 1 bottle); D reagent: enzyme-labeled antigen diluent (1 bottle); E reagent: concentrated washing solution (1 bottle); F reagent: chromogenic substrate solution a (1 bottle); G reagent: chromogenic substrate solution b (1 bottle); H ...

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Abstract

The invention relates to a novel detection method of nanogold labeling-aflatoxin B1, which utilizes nanogold labeling-aflatoxin B1 (AFB1), combines nanogold silver enhancement, absorbance detection / digestion chemiluminescence detection technology and builds two AFB1 novel ultrasensitive detection systems. In the first method, an AFB1 antibody, gold-labelled antigen and antigen to be detected carry out competitive immunometric reaction; then, silver enhancement solution is added, and golden is taken as a core to deposit and grow silver; the absorbance is detected to determine the content of AFB1 in an object to be measured; the detection limit of the method can reach 0.01ng / mL. In the second method which is on the basis of the first method, silver is chemically dissolved out; the amount of deposited silver is detected with the chemiluminesence immunoassay to determine the content of AFB1 in the object to be measured; the detection limit of the method can reach 0.002ng / mL. The AFB1 detection technology built by the invention is accurate, sensitive and quick and has important meaning for food safety.

Description

technical field [0001] The invention relates to the use of nano-gold to label aflatoxin B 1 (AFB 1 ) artificial antigen, using the principle of nano-gold and silver enhancement, combined with absorbance detection / dissolution chemiluminescence detection technology, established AFB 1 Novel ultrasensitive detection system. The invention belongs to the technical field of biological cytology and the detection method of toxic metabolites of microbial fungi. Background technique [0002] Aflatoxin B 1 (AFB 1 ) is a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus, which is extremely toxic and carcinogenic, and can cause liver cancer, gastric cancer, kidney cancer, etc. in animals. It is the strongest chemical carcinogen found so far. It can also pass The food chain is transferred between organisms, thus not only causing economic losses to farmers and poultry and livestock producers, but also contaminating food and then posing a certain threat to human hea...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/31G01N21/76G01N33/532
Inventor 王周平李井泉段诺吴世嘉
Owner JIANGNAN UNIV
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