Extraction method of the tobacco thin slice production of paper process
A technology of tobacco flakes and papermaking, which is applied in the fields of tobacco, tobacco preparation, and tobacco treatment. It can solve the problems of no application of acid protease, heavy irritation, and poor quality of flakes, and achieve obvious sweetness and reduced irritation of tobacco. , The effect of reducing the miscellaneous gas
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Embodiment 1
[0045](1) liquid submerged fermentation prepares acid protease: 100 liters of fermenters, dress 60 liters of culture medium, sterilize at 121 ℃ for 30 minutes, cool, inoculate 1.0 * 10 spores, in 250 rev / mins, in control ventilation (V Wind / V solution·time): 0-24 hours 1: 0.4, 24-60 hours 1: 0.7, control the culture temperature at 30°C, and ferment for 60 hours. The composition of the culture medium is in parts by weight: 5.0 parts of bean cake powder, 2.0 parts of bran, 0.3 parts of NH4Cl, 0.03 parts of Na2HPO, 0.06 parts of KH2PO and 100 parts of water.
[0046] (2) Post-treatment of acid protease fermented liquid: the fermented liquid is vacuum-concentrated at a pressure of 0.8 × 104Pa and a temperature of 40°C, concentrated to 10% of the original volume, and then vacuumed at a pressure of 0.7 × 104Pa and a temperature of 40°C drying, drying to a moisture content of ≤5%, and grinding at low temperature to obtain an acidic protease preparation.
[0047] (3) Weigh 800 kg of ...
Embodiment 2
[0050] (1) Preparation of acid protease by liquid submerged fermentation: 100 liters of fermenter, 50 liters of medium, sterilized at 121°C for 30 minutes, cooled, inoculated with 2.0×10 12 spores, at 200 rpm, under controlled ventilation (V 风 / V 液 · Time): 0-24 hours 1:0.3, 24-72 hours 1:0.6, control the culture temperature at 35° C., and ferment for 72 hours. The composition of the medium is in parts by weight: 5.0 parts of bean cake powder, 3.0 parts of bran, NH 4 0.4 parts of Cl, Na 2 HPO 4 0.03 parts, KH 2 PO 4 0.08 parts and 100 parts of water.
[0051] (2) post-treatment of acidic protease fermented liquid: the fermented liquid is treated at a pressure of 1.0×10 4 Pa, under the condition of 50℃, carry out vacuum concentration, concentrate to 15% of the original volume, and then under the pressure of 0.6×10 4 Pa, vacuum drying under the condition of 35° C., drying to a moisture content of ≤5%, and grinding at low temperature to obtain an acidic protease preparati...
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