Process for efficiently concentrating cordycepin

A technology of Cordyceps mycelium and process, which is applied in the directions of medical preparations containing active ingredients, antibacterial drugs, plant/algae/fungus/moss ingredients, etc. Effect

Active Publication Date: 2010-07-07
NANJING ZHONGKE PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there are reports from Cordyceps mycelium to extract Cordyceps polysaccharides through water extraction and alcohol precipitation process or to extract cordycepin from them through alcohol extraction process, but both are complicated and cumbersome and the yield is low.

Method used

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  • Process for efficiently concentrating cordycepin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Get artificial Cordyceps militaris powder 10kg.

[0055] a. Ultrasonic extraction:

[0056] The ultrasonic temperature is 25°C, the extract is 30% ethanol aqueous solution, the ultrasonic time is 25min, the mass-volume ratio of artificial Cordyceps militaris to the extract is 1:20kg / L, the power of the ultrasonic equipment is 20kw, and the volume of the extraction tank is The ratio is 20kw / m 3 .

[0057] b. Membrane filtration concentration:

[0058] Take the above-mentioned ultrasonic concentrated solution of Cordyceps, control the temperature at 20°C, and perform ultrafiltration. The ultrafiltration membrane adopts a polyamide roll-type membrane with a molecular weight cut-off of 20,000-40,000 Daltons, and the pressure is controlled at 0.4-0.6MPa. Ultrafiltration is performed for 3.5 hours to obtain The concentrated solution after ultrafiltration has a volume of 40% of the original extract.

[0059] c. Freeze drying:

[0060] The concentrated solution after ultra...

Embodiment 2

[0062] Get artificial Cordyceps militaris powder 10kg.

[0063] a. Ultrasonic extraction:

[0064] The ultrasonic temperature is 25°C, the extract is 50% ethanol aqueous solution, the ultrasonic time is 50min, the mass-volume ratio of the artificial Cordyceps militaris to the extract is 1:10kg / L, the power of the ultrasonic equipment is 10kw, and the volume of the extraction tank is The ratio is 10kw / m 3 .

[0065] b. Membrane filtration concentration:

[0066] Take the above-mentioned ultrasonic concentrated solution of Cordyceps, control the temperature at 22°C, and perform ultrafiltration. The ultrafiltration membrane adopts a polyamide roll-type membrane with a molecular weight cut-off of 20,000-40,000 Daltons, and the pressure is controlled at 0.4-0.6MPa. Ultrafiltration is performed for 3.5 hours to obtain The concentrated solution after ultrafiltration has a volume of 30% of the original extract.

[0067] c. Freeze drying:

[0068] The concentrated solution after u...

Embodiment 3

[0070] Get artificial Cordyceps militaris powder 10kg.

[0071] a. Ultrasonic extraction:

[0072] The ultrasonic temperature is 20°C, the extract is 40% ethanol aqueous solution, the ultrasonic time is 75min, the mass-volume ratio of artificial Cordyceps militaris to the extract is 1:30kg / L, the power of the ultrasonic equipment is 30kw, and the volume of the extraction tank is The ratio is 30kw / m 3 .

[0073] b. Membrane filtration concentration:

[0074] Take the above-mentioned ultrasonic concentrated solution of Cordyceps, control the temperature at 24°C, and perform ultrafiltration. The ultrafiltration membrane adopts a polyamide roll-type membrane with a molecular weight cut-off of 20,000-40,000 Daltons, and the pressure is controlled at 0.4-0.6MPa. Ultrafiltration is performed for 3.5 hours to obtain The concentrated solution after ultrafiltration has a volume of 50% of the original extract.

[0075] c. Freeze drying:

[0076] The concentrated solution after ultra...

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Abstract

The invention discloses a process for efficiently concentrating cordycepin, wherein ultrasound is utilized to obtain ultrasonic concentrated solution through extraction. The conditions are as follows: the ultrasonic temperature is 20-30 DEG C; the mass percentage concentration used by the extracting liquid is ethanol water of 30-50%; the ultrasonic time is 25-75min; the mass and volume ratio of artificial cordyceps militaris to extracting liquid is 1:10-30kg/L; the volume ratio of ultrasonic power to an extraction tank is 10-30kw/m<3>; then the polyamide rolled membrane with the cut-off molecular weight of 20000-40000 Daltons is used for concentration; and the obtained concentration liquid is frozen and dried for 35-40h under the conditions that the temperature is less than or equal to 30 DEG C and the vacuum degree is 5-10Pa. In the concentration frozen powder obtained by concentrating cordycepin by the method in the invention, the main effective components are obviously improved.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a process for efficiently concentrating Cordyceps mycelium. Background technique [0002] Cordyceps sinensis, also known as Cordyceps sinensis, is a medicinal fungus of the genus Cordyceps in the family Ergotaceae. It is mainly produced in mountains and meadows at an altitude of 4000m to 5000m in Qinghai, Tibet, Sichuan, Yunnan and Guizhou. It is a complex produced by the subunit and larvae of Cordyceps sinensis sall parasitizing on the larvae of Hepfalus armoricanus oberthur, an insect of the family Batidae. [0003] As early as the Qing Dynasty, Wu Yiluo had a detailed record of Cordyceps sinensis in "Materia Medica Congxin": "Winter in the soil, the body lives like an old silkworm, with hair that can move, and the summer solstice, the hair emerges from the soil, and the whole body turns into grass." ; Its nature and flavor functions are: "sweet, flat"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61P37/04A61P29/00A61P31/04A61P31/12A61P35/00A61P39/06A61P25/00A61P11/00A61P7/02A61P9/06A61P9/10A61P9/12
Inventor 冯敏沈建钱一帆
Owner NANJING ZHONGKE PHARMA
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