Method for detecting aspergillus by polymerase chain reaction
A technology of chain reaction and Aspergillus, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to reduce the possibility of pollution, save DNA extraction steps, and achieve good repeatability
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[0034] 1. Treatment of Aspergillus niger
[0035] 1. The standard Aspergillus niger strain was inoculated with Cha's medium and cultured at 37°C for 3 days, and the colony was picked and inoculated in LB liquid medium, shaken overnight at 37°C, and the hyphae were collected. Weigh 0.05g of mycelia into a 1.5ml plastic centrifuge tube, add 200uL 2% cellulase (prepared with 0.65mol / L NaCl), after 3 hours of oven treatment at 37°C, add 20uL of 20mg / ml proteinase K, and bathe in water at 55°C for 2 hours Finally, add 1% TritonX-100. Put the centrifuge tube into liquid nitrogen to freeze for 1 minute, insert it into boiling water for 1 minute, put it into liquid nitrogen to freeze it for 1 minute, insert it into boiling water for 1 minute, put it into liquid nitrogen and freeze it for 1 minute, and finally insert it into boiling water for 10 minutes.
[0036] 2. The standard Aspergillus niger strain was inoculated with Cha's medium and cultured at 37°C for 3 days, and the colony w...
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