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Pinecone extractive of Yunnan Pinus and preparation method and medicinal application thereof

A technology of Yunnan pine and extract, which is applied in the field of medicine, can solve the problems of no pine cone anti-HIV activity and complicated process, and achieve the effect of stable composition and drug effect, simple extraction process, and industrialization

Inactive Publication Date: 2010-07-14
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to Chinese and foreign literature search and query, except that Japanese scholars proposed that Japanese white pine pine cones have anti-HIV effects, there are no relevant reports that pine cones derived from other tree species have anti-HIV activity
In addition, Japanese scholars believe that the anti-HIV active site of the Japanese white pine cone is a macromolecular acidic polysaccharide, and its extraction method is repeated use of alkaline water extraction, which has a complicated process

Method used

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  • Pinecone extractive of Yunnan Pinus and preparation method and medicinal application thereof
  • Pinecone extractive of Yunnan Pinus and preparation method and medicinal application thereof
  • Pinecone extractive of Yunnan Pinus and preparation method and medicinal application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 : Preparation of Pine Cone Extract H-D of Pinus yunnanensis

[0025] 10 kg of dried pine cones of Huashan pine were crushed, soaked in 20 liters of 95% ethanol solution for 24 hours × 3 times, and filtered. The residue was extracted with boiling water for 2 hours x 3 times, then extracted with 1% sodium hydroxide solution at room temperature, and filtered. The filtrate was adjusted to pH 5 with acetic acid solution and filtered. The filtrate was precipitated with multiple volumes of ethanol solution and filtered. The precipitate was washed with absolute ethanol, and dried under reduced pressure at 40° C. to obtain 7.52 g of pine cone extract of Yunnan Huashan pine (sample number: H-D).

Embodiment 2

[0026] Example 2 : Preparation of Pine Cone Extract H-F of Pinus yunnanensis

[0027] 10 kg of dried pine cones of Huashan pine, crushed, soaked in 20 liters of 95% ethanol solution for 3 hours × 3 times, and filtered. The residue was extracted with boiling water for 2 hours x 3 times, then extracted with 1% sodium hydroxide solution at room temperature, and filtered. The filtrate was adjusted to pH 5 with acetic acid solution and filtered. The filtrate was precipitated with multiple volumes of ethanol solution and filtered. The filtrate was precipitated with 2 times the amount of ethanol solution and filtered. The filtrate was then precipitated with 5 times the amount of ethanol solution and filtered. The precipitate was washed successively with absolute ethanol and ether, and dried under reduced pressure at 40° C. to obtain 3.83 g of pine cone extract of Yunnan Huashan pine (sample number: H-F).

Embodiment 3

[0028] Example 3 : Preparation of Pine Cone Extract Y-E of Yunnan Pinus

[0029] 10 kg of dried pine cones of Yunnan pine, crushed, soaked in 20 liters of 95% ethanol solution for 24 hours × 3 times, and filtered. The residue was extracted with boiling water for 2 hours x 3 times, then extracted with 1% sodium hydroxide solution at room temperature, and filtered. The filtrate was adjusted to pH 5 with acetic acid solution and filtered. The filtrate was precipitated with multiple volumes of ethanol solution and filtered. The filtrate was precipitated with 2 times the amount of ethanol solution and filtered. The precipitate was washed with absolute ethanol, and dried under reduced pressure at 50° C. to obtain 3.96 g of pine cone extract of Pine yunnanensis (sample number: Y-E).

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Abstract

The invention discloses a pinecone extractive of Yunnan Pinus and a preparation method and medicinal application thereof, and relates to a preparation method of a pinecone extractive of Pinus armandi, pinus yunnanensis, Himalayan pine and / or pinus khasys of Yunnan Pinus and medicinal application thereof. The pinecone extractive of Yunnan Pinus is prepared by the following steps of: soaking crushed pinecones by an alcohol solution, extracting with hot water and then carrying out alcohol-precipitation extracting by a filtrate extracted by water containing soda at room temperature. The pinecone extractive of Yunnan Pinus can effectively restrain AIDS viruses (HIV) and can be expected to be developed into a drug for preventing and controlling aids. The results of pharmacodynamics tests show that the pinecone extractive of Yunnan Pinus has the in vitro anti-HIV-1 activity, and the therapeutic index of the pinecone extractive is larger than 50 (59.99 to 515.48).

Description

technical field [0001] The invention relates to the technical field of medicine. Specifically, the present invention relates to the pine cone extract of plants of the genus Yunnan Pinus and its medicinal use. The extract exhibits an effective effect of inhibiting HIV replication in vitro, has a high therapeutic index, and can be expected to be developed into a drug for preventing and treating AIDS. Background technique [0002] The full medical name of AIDS is "Acquired Immune Deficiency Syndrome" (Acquired Immune Deficiency Syndrome, AIDS), which is caused by human immunodeficiency virus (HIV, also known as AIDS virus). HIV virus is a virus that can attack the human immune system. It targets the most important T4 lymphocytes in the human immune system, engulfs and destroys T4 lymphocytes in large quantities, thereby destroying the human immune system and eventually causing the immune system to collapse. It makes the human body fall ill due to the loss of resistance to var...

Claims

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Application Information

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IPC IPC(8): A61K36/15A61P31/18A61K131/00
Inventor 刘光明赵昱吕永俊李好枝彭芳张桢李冬梅罗建蓉巫秀美周萍杨敏张海珠张兰胜施贵荣李春艳董光平
Owner DALI UNIV
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