Method for synthesizing high-molecular-weight polypeptide through catalysis by using hydroborated rare earth as catalyst

A technology of rare earth borohydride and high molecular weight, which is used in the field of rare earth borohydride catalysts to catalyze the synthesis of high molecular weight polypeptides. It can solve the problems of extremely high purity requirements, cumbersome steps, and the inability to realize industrialization, and achieve high activity and narrow molecular weight distribution. Effect

Inactive Publication Date: 2010-07-14
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

3) In 2004, N. Hadjichristidis et al. [3] Using the traditional primary amine as the initiator, the living polymerization of NCA was realized by using a high vacuum system. Although this method obtains a polypeptide with a narrow molecular weight distribution, it requires extremely high purity of the NCA monomer and requires the application of a special High vacuum line to carry out the polymerization reaction, the steps are extremely cumbersome, the operation is extremely complicated, and it cannot be industrialized

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  • Method for synthesizing high-molecular-weight polypeptide through catalysis by using hydroborated rare earth as catalyst

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Embodiment 1

[0012] A method for catalytically synthesizing high-molecular-weight polypeptides using borohydride rare earth as a catalyst, characterized in that: borohydride rare earth Ln(BH 4 ) 3 As a catalyst, catalyzing the ring-opening polymerization of NCA monomers to obtain high molecular weight polypeptides; Ln in the catalyst is a rare earth element Sc, NCA monomers adopt BLG-NCA monomers, BLG-NCA monomers and Sc(BH 4 ) 3 The molar ratio was 20:1, the polymerization reaction temperature was -30°C, and the polymerization reaction time was 3 days. The yield of the obtained polypeptide is 83%, and the molecular weight is carried out in a Ubbelohde viscometer using the viscosity method, using dichloroacetic acid as a solvent, measured at 25°C, and the molecular weight is determined by the formula [η]=2.78×10 -5 m 0.87 calculate [6] , whose value is 29,000. The absolute molecular weight and molecular weight distribution of the polymer were determined by gel permeation chromatograph...

Embodiment 2

[0014] A method for catalytically synthesizing high-molecular-weight polypeptides using borohydride rare earth as a catalyst, characterized in that: borohydride rare earth Ln(BH 4 ) 3 As a catalyst, catalyzing the ring-opening polymerization of NCA monomers to obtain high molecular weight polypeptides; the Ln in the catalyst is a rare earth element Y, and the NCA monomers adopt BLG-NCA monomers, and the BLG-NCA monomers and Y(BH 4 ) 3 The molar ratio was 400:1, the polymerization temperature was 0°C, and the polymerization time was 2.5 days. The yield of the obtained polypeptide is 88%, and the molecular weight is carried out in a Ubbelohde viscometer using the viscosity method, using dichloroacetic acid as a solvent, measured at 25°C, and the molecular weight is determined by the formula [η]=2.78×10 -5 m 0.87 calculate [6] , whose value is 66,000. The absolute molecular weight and molecular weight distribution of the polymer were measured using gel permeation chromatogra...

Embodiment 3

[0016] A method for catalytically synthesizing high-molecular-weight polypeptides using borohydride rare earth as a catalyst, characterized in that: borohydride rare earth Ln(BH 4 ) 3 As a catalyst, it catalyzes the ring-opening polymerization of NCA monomers to obtain high-molecular-weight polypeptides; the Ln in the catalyst is the rare earth element La, and the NCA monomer adopts BLL-NCA monomer, and the BLL-NCA monomer and La(BH 4 ) 3 The molar ratio is 800:1, the polymerization reaction temperature is 20° C., and the polymerization reaction time is 2.0 days. The yield of the obtained polypeptide is 95%, and the molecular weight is carried out in a Ubbelohde viscometer using the viscosity method. With dichloroacetic acid as the solvent, it is measured at 25°C. The molecular weight is determined by the formula [η]=2.78×10 -5 m 0.87 calculate [6] , whose value is 79,000. The absolute molecular weight and molecular weight distribution of the polymer were measured using g...

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Abstract

The invention relates to a method for synthesizing a high-molecular-weight polypeptide through catalysis by using hydroborated rare earth as a catalyst, which is characterized by comprising the following steps: synthesizing an alpha-amino acid-N-carboxyl anhydride monomer by using amino acid and triphosgene as initial raw materials, and catalyzing the alpha-amino acid-N-carboxyl anhydride monomer to carry out ring opening polymerization by using the hydroborated rare earth Ln(BH4)3 as the catalyst to obtain the high-molecular-weight polypeptide. Compared with prior art, the invention has the following outstanding advantages: compared with other catalysts, the catalyst used by the invention is single-component and homogeneous, and the catalyst has the following characteristics: 1. the catalyst is cheap and easy to obtain and has high activity; 2. the high-molecular-weight polypeptide can be prepared by using the catalyst, and the weight average molecular weight can be higher than 120 thousand; 3. the prepared polypeptide has narrow molecular weight distribution (not more than 1.5); and 4. polypeptides in different types can be synthesized, and the copolymerization among different polypeptides also can be realized.

Description

technical field [0001] The invention relates to a method for obtaining a polypeptide by using a rare earth catalyst to catalyze the ring-opening polymerization of α-amino acid-N-carboxy anhydride (NCA) monomers, in particular to a method for catalyzing the synthesis of high-molecular weight polypeptides by using rare earth borohydride as a catalyst. Background technique [0002] Polypeptide is the most biocompatible material among many biomedical materials, and its application has attracted the attention of various research institutions around the world. There are three methods for chemical synthesis of peptides: (1) solid-phase synthesis; (2) solution coupling polymerization; (3) NCA ring-opening polymerization. Among them, the most promising method for synthesizing high molecular weight peptides is NCA ring-opening polymerization. The research reports on catalysts that catalyze NCA ring-opening polymerization to obtain peptides mainly include: 1) Traditional amine initiat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/16
Inventor 凌君
Owner ZHEJIANG UNIV
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