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Treatment of urinary tract infections with a mixture of saponin and an antibiotic

A technique for urinary infection and antibiotics, applied to urinary system diseases, vector-borne diseases, drug delivery, etc., can solve problems such as infection

Inactive Publication Date: 2010-07-21
COLOPLAST AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, patients who use catheters to empty their bladders are also highly susceptible to infection, with a risk of catheter-related UTIs of approximately 1-5% per day of catheter use (Wazait et al., 2003)

Method used

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  • Treatment of urinary tract infections with a mixture of saponin and an antibiotic
  • Treatment of urinary tract infections with a mixture of saponin and an antibiotic
  • Treatment of urinary tract infections with a mixture of saponin and an antibiotic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Seven different strains of wild-type E. coli bacteria were isolated from UTI patients and cultured in LuriaBroth (LB) medium under static conditions for 2 days. Methods of culturing bacteria are familiar to those skilled in the art of microbiology. The 2-day-old bacteria were diluted 100-fold in fresh sterile LB, with different concentrations of gentamicin or no gentamicin (control). Gentamicin is used as a representative antibiotic agent. The bacteria were then allowed to grow for an additional 2 hours under optimal growth conditions (shaking, 37°C) and the actual concentration of bacterial cells in the LB was measured by measuring absorbance at 600 nm. Absorbance reflects the number of bacteria. The number of bacteria is expressed relative to a control preparation without gentamicin. as image 3 As shown in the figure above, the number of bacterial cells is reduced at very low concentrations of gentamicin, showing that gentamicin is very effective in inhibiting th...

Embodiment 2

[0049] Human bladder cells were cultured and then infected with bacteria. Briefly, human bladder cells (HTB-9 from the American Tissue Culture Collection) were cultured in medium and CO 2 Incubator at 37°C until the actual experiment. The cultivation of human cells is familiar to those skilled in the biological arts. Next, varying concentrations of E. coli were added to human bladder cells. Bladder cells and bacteria were allowed to incubate together for 1 or 2 hours, then most of the loosely bound and extracellular E. coli cells (human bladder cells attached to the bottom of the cell culture flask) were removed by washing the bladder cells with PBS. Human bladder cells were exposed to 100.0 μg / ml gentamicin for 2 hours. Under normal conditions, gentamicin cannot pass through any membranes. As already shown in Example 1, the gentamicin treatment will kill all bacteria if gentamicin is accessible to the bacteria. Residual gentamicin and dead bacteria were removed by washin...

Embodiment 3

[0051] The green-stained bacteria were also infected with human bladder cells as described in Example 2 above. Infected bladder cells were then treated with ethidium bromide as explained in detail by Drevets and Campbell 1991 . Ethidium bromide will turn green-stained bacteria yellow / red if it has access to the bacteria. This experimental method allows the visual detection by microscopy of green bacteria that are not stained with ethidium bromide inside the cell as well as bacteria that are stained red / yellow outside the cell. as Figure 5 As shown, only the green-stained bacteria were located inside the bladder cells (enclosed by white lines), while the yellow / red-stained bacteria were found outside or near the human bladder cells.

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Abstract

Device for treating urinary infections comprising a catheter and a container, wherein the container comprises a cholesterol-binding agent. Patients who are using a urinary catheter to empty the bladder of urine are very prone to get an infection and the risk of getting a catheter associated Urinary Tract Infections (UTI) is high. The introduction of a cholesterol-binding agent facilitates a more effective curing of the infection.

Description

field of invention [0001] The present invention discloses examples of intracellular bacteria that heal bladder cells and uses Escherichia Coli as a representative test bacterium. The present invention is not limited to the treatment of recurrent urinary tract infections caused only by E. coli (hereinafter referred to as recurrent UTI), but may include recurrent UTI caused by all types of microorganisms, such as bacteria, fungi or viruses. Background of the invention [0002] The urinary tract is one of the main sites for microbial colonization. UTI is the most common bacterial infection of all organs. The extent of this is evidenced by the number of people seeking medical care, which is estimated to be about 8 million per year in the United States. Furthermore, patients who use urinary catheters to empty their bladders of urine are also highly susceptible to infection, with a risk of catheter-associated UTIs of approximately 1-5% per day of catheter use (Wazait et al., 200...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P31/02
CPCA61K31/704A61K9/0034A61P13/02A61P31/02Y02A50/30
Inventor 瑟伦·克里斯蒂安森
Owner COLOPLAST AS
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