Pasty biological pesticide composite and preparation method thereof
A technology for biological pesticides and compositions, which is applied in the fields of botanical equipment and methods, chemicals used for biological control, biocides, etc., can solve problems such as insufficient storage time and insufficient stability of preparation quality, and is beneficial to environmental protection. , The effect of stable product quality and long storage time
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Embodiment 1
[0043] Embodiment 1, Trichoderma liquid medium formula: sugar beet liquid 0~20.0g, (NH 4 ) 2 SO 4 0~2.0g, MgSO 4 ·7H 2 O 0~0.2g, KH 2 PO 4 0~2.0g, 0~10.0g sucrose;
[0044] Biopesticide composition in paste form and preparation method thereof: the ingredients of the invention include: Trichoderma hyphae, Trichoderma chlamydospores, hydrochloric acid, potato starch and copper ions.
[0045] Adopt Trichoderma liquid culture medium formula 1: sugar beet liquid 20.0g, (NH 4 ) 2 SO 4 0g, MgSO 4 ·7H 2 O0.2g, KH 2 PO 4 2.0g, sucrose 0g;
[0046] Trichoderma was inoculated by 5% inoculum, at 26 ° C, after 36 hours of shake cultivation at 150 rpm / min, filtered, the mycelium after filtering was taken out, the pH of the mycelia was adjusted to 3.5 with hydrochloric acid, and a small amount of copper ion inhibitor was added, and then Add 10% potato starch and stir evenly to prepare the bio-pesticide in paste form. The preparation process needs to be operated under sterile c...
Embodiment 2
[0047] Example 2 is basically the same as Example 1, but inoculated into a sterile liquid medium with an inoculum size of 2%; shake cultured at 28°C for 48h, after the filtered mycelium was taken out, the pH of the mycelia was adjusted to 3.0, add a small amount of copper ion inhibitor, and then add 8% potato starch.
[0048] Trichoderma liquid medium formula is: sugar beet liquid 20.0g, (NH 4 ) 2 SO 4 0g, MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 2.0g, 0g of sucrose.
Embodiment 3
[0049]Example 3 is basically the same as Example 1, but is inoculated into a sterile liquid medium with an inoculum size of 10%; at 27° C., shake culture for 40 h, after the filtered mycelium is taken out, adjust the pH of the mycelia with hydrochloric acid To 4.0, add a small amount of copper ion inhibitor, and then add 5% potato starch.
[0050] Trichoderma liquid medium formula is: sugar beet liquid 20.0g, (NH 4 ) 2 SO 4 0g, MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 2.0g, 5.0g of sucrose.
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