Batch-type groundwater denitrifying method
A groundwater and denitrification technology, applied in chemical instruments and methods, contaminated groundwater/leachate treatment, water pollutants, etc. Pollution, good application prospects, the effect of avoiding pollution
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Embodiment 1
[0040] See attached figure 1 , a kind of intermittent groundwater denitrification denitrification method, comprises the following steps:
[0041] (1) Enrichment culture of denitrifying bacteria
[0042] Add KNO separately to each liter of distilled water 3 2000mg / L, C 2 h 5 OH 1500mg / L, CaCl 2 350mg / L, MgSO 4 ·7H 2 O 300mg / L, 0.5mL of 0.2mol / L phosphate buffer solution of pH 7.2 constitutes denitrification bacteria enrichment culture solution; Add activated sludge from urban domestic sewage treatment plant into denitrification bacteria culture solution to carry out denitrification Enrichment culture of nitrifying bacteria; ordinary stirring reactor with intermittent operation is used for stirring reaction for 20 hours a day, static sedimentation for 4 hours, and the supernatant is discharged by siphon method, and then fresh denitrifying bacteria culture solution is added for the next round of operation. Run for 70 days to get denitrifying bacteria suspension.
[0043...
Embodiment 2
[0052] A kind of intermittent groundwater denitrification denitrification method, basic steps are the same as embodiment 1, difference is (see attached figure 1 ):
[0053] Pour out the simulated groundwater 1 in the middle chamber 11 of the reactor in Example 1 and the ethanol aqueous solution 2 in the left chamber 12 and the right chamber 13, and add 900mL in the middle chamber 11 In the simulated groundwater 1, 900mL of distilled water and 60mg of 95% ethanol (the COD of ethanol aqueous solution 2) are respectively added in the left chamber 12 and the right chamber 13 Cr Concentration is about 132mg / L), repeat each step operation of embodiment 1.
[0054] After 36 hours of reaction, the NO in the intermediate chamber 11 3 - -N and COD Mn The concentrations are 8.9mg / L and 2.9mg / L respectively, that is, nitrate nitrogen and COD Mn All meet the requirements of China's "Drinking Water Sanitation Standard" (GB5749-2006). At the same time, the NO in the left chamber 12 an...
Embodiment 3
[0056] See attached figure 2 , an intermittent groundwater denitrification denitrification method, its specific operation steps are:
[0057] Steps (1) to (3) are the same as in Example 1.
[0058] (4) Construction of immobilized cell membrane bioreactor
[0059] In step (3), the surface of each plate-shaped fixed cell 3 side obtained in step (3) is covered with a microporous filter membrane 4 with a pore size of 0.45 μm, and then the plate-shaped fixed cells 3 and the microporous filter membrane 4 are fixed with flange 9 and bolts fixed together figure 2 In the shown reactor housing 5, a flat plate-shaped fixed cell bioreactor is obtained.
[0060] (5) Groundwater denitrification and denitrification
[0061] use NaNO 3 Prepare with distilled water The simulated groundwater 1, in figure 2 900mL simulated groundwater is respectively added in the left side chamber 12 and the right side chamber 13 of the shown reactor, and 900mL distilled water and 130mg 95% ethanol ar...
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