Process for extracting blood sugar reducing active saponin from cyclocarya paliurus

Abstract

The invention relates to a process for extracting blood sugar reducing active saponin from cyclocarya paliurus. The invention adopts the technical scheme that the method comprises the following steps: putting dry cyclocarya paliurus leaves into a flask, extracting the leaves under the refluxing of 90 percent ethanol solution, then centrifuging or filtering the extract, and reclaiming the ethanol solvent under reduced pressure when the filtered solution is at 58 DEG C to obtain dry cyclocarya paliurus paste with the density of 1.15 grams per cubic centimeter; dissolving 1 gram of dry cyclocarya paliurus paste into 3 milliliters of distilled water, transferring the solution into a separating funnel, extracting by using petroleum ether to remove grease, extracting the solution by using ethyl ether, combining water phases, then extracting by using n-butanol, combining n-butanol solution, and concentrating the solution under reduced pressure till the solution is reduced; and dissolving the concentrated substance in the distilled water, filtering the solution, filling the solution on an HPD400 macroporous resin column, removing sugar impurities by using the distilled water, eluting the macroporous resin column by using 70 percent ethanol, collecting 70 percent ethanol elution, concentrating the elution under reduced pressure till the elution is dried so as to obtain hederagenin H, wherein the yield of the saponin is 1.258 percent through measurement. The yield of the hederagenin obtained by the method is higher.

Description

Technical field:

[0001] The invention relates to a process for extracting blood sugar-lowering active saponins from Cyclocarya paliurus. Background technique:

[0002] Cyclocarya paliurus (Batal.) Ijinsk is a plant of the genus Cyclocarya paliurus (Batal.) Ijinsk, which is one of the endangered plants under national key protection. It is a tall and fast-growing tree widely distributed in Jiangxi, Zhejiang, Hunan, Guangdong, etc. The area south of the Yangtze River (document 1 has specific reports). According to the records of "Chinese Materia Medica", it has the functions of expelling wind, reducing inflammation and relieving pain, killing parasites and relieving itching. The folks have also found that it has the effects of clearing away heat and detoxification, promoting body fluid to quench thirst, lowering blood pressure and strengthening the heart, so as to prolong life (document 2 has specific reports). Its main components are saponins, flavonoids, terpenoids, polysac...

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