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Process for extracting blood sugar reducing active saponin from cyclocarya paliurus

An extraction process and hypoglycemic technology, applied in the fields of steroids, organic chemistry, etc., can solve the problem that the extraction process has not been reported.

Inactive Publication Date: 2013-11-13
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, although the hypoglycemic effect of Cyclocarya saponin is more certain, there is no report on the extraction process of Cyclocarya saponin with hypoglycemic activity

Method used

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  • Process for extracting blood sugar reducing active saponin from cyclocarya paliurus

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1, the extraction process of Cyclocarya paliurus hypoglycemic active saponins, wherein:

[0015] Weigh 20.0 grams of dry leaves of Cyclocarya paliurus, put them in a flask, and extract once with 220 grams of ethanol solution with a volume concentration of 90%, for 3 hours each time; then centrifuge or filter the extract, and recover the filtrate under reduced pressure at 58°C Obtaining the Cyclocarya paliurus dry paste with a density of 1.15 grams / cubic centimeter after the ethanol solvent;

[0016] Take 1 g of Cyclocarya paliurus dry paste and add 3 ml of distilled water to dissolve it, transfer it to a separatory funnel, use 3 ml of petroleum ether for degreasing, and then use 3 ml of ether to divide it into an even amount. Extract 4 times, combine the water phase, and then extract 5 times with 5 ml of n-butanol according to the uniform amount, combine the n-butanol liquid, concentrate to dryness under reduced pressure; add 3 ml of distilled water to dissol...

Embodiment 2

[0017] Embodiment 2, the extraction process of Cyclocarya paliurus hypoglycemic active saponins, wherein:

[0018] Weigh 20.0 grams of dry leaves of Cyclocarya paliurus, put them in a flask, and use 230 grams of ethanol solution with a volume concentration of 90% to reflux and extract twice, each time for 2 hours; then the extract is centrifuged or filtered, and the filtrate is recovered under reduced pressure at 58°C Obtaining the Cyclocarya paliurus dry paste with a density of 1.15 grams / cubic centimeter after the ethanol solvent;

[0019] Take 1 g of Cyclocarya paliurus dry paste and add 4 ml of distilled water to dissolve it, transfer it to a separatory funnel, first use 4 ml of petroleum ether to extract it in 5 times in an even amount for degreasing, and then use 4 ml of ether to divide it in an even amount Extract 4 times, combine the water phase, and then extract 5 times with 6 ml of n-butanol according to the uniform amount, combine the n-butanol liquid, concentrate t...

Embodiment 3

[0020] Embodiment 3, the extraction process of Cyclocarya paliurus hypoglycemic active saponins, wherein:

[0021] Weigh 20.0 grams of dry leaves of Cyclocarya paliurus, put them in a flask, and use 240 grams of ethanol solution with a volume concentration of 90% to reflux extract 3 times, each time for 1 hour; then centrifuge or filter the extract, and recover the filtrate under reduced pressure at 58°C Obtaining the Cyclocarya paliurus dry paste with a density of 1.15 grams / cubic centimeter after the ethanol solvent;

[0022] Take 1 g of Cyclocarya paliurus dry paste and add 5 ml of distilled water to dissolve it, transfer it to a separatory funnel, first use 5 ml of petroleum ether to extract 5 times in an even amount for degreasing, and then use 5 ml of ether to divide it into an even amount. Extract 4 times, combine the water phase, and then extract 5 times with 7 ml of n-butanol according to the uniform amount, combine the n-butanol liquid, concentrate to dryness under r...

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Abstract

The invention relates to a process for extracting blood sugar reducing active saponin from cyclocarya paliurus. The invention adopts the technical scheme that the method comprises the following steps: putting dry cyclocarya paliurus leaves into a flask, extracting the leaves under the refluxing of 90 percent ethanol solution, then centrifuging or filtering the extract, and reclaiming the ethanol solvent under reduced pressure when the filtered solution is at 58 DEG C to obtain dry cyclocarya paliurus paste with the density of 1.15 grams per cubic centimeter; dissolving 1 gram of dry cyclocarya paliurus paste into 3 milliliters of distilled water, transferring the solution into a separating funnel, extracting by using petroleum ether to remove grease, extracting the solution by using ethyl ether, combining water phases, then extracting by using n-butanol, combining n-butanol solution, and concentrating the solution under reduced pressure till the solution is reduced; and dissolving the concentrated substance in the distilled water, filtering the solution, filling the solution on an HPD400 macroporous resin column, removing sugar impurities by using the distilled water, eluting the macroporous resin column by using 70 percent ethanol, collecting 70 percent ethanol elution, concentrating the elution under reduced pressure till the elution is dried so as to obtain hederagenin H, wherein the yield of the saponin is 1.258 percent through measurement. The yield of the hederagenin obtained by the method is higher.

Description

Technical field: [0001] The invention relates to a process for extracting blood sugar-lowering active saponins from Cyclocarya paliurus. Background technique: [0002] Cyclocarya paliurus (Batal.) Ijinsk is a plant of the genus Cyclocarya paliurus (Batal.) Ijinsk, which is one of the endangered plants under national key protection. It is a tall and fast-growing tree widely distributed in Jiangxi, Zhejiang, Hunan, Guangdong, etc. The area south of the Yangtze River (document 1 has specific reports). According to the records of "Chinese Materia Medica", it has the functions of expelling wind, reducing inflammation and relieving pain, killing parasites and relieving itching. The folks have also found that it has the effects of clearing away heat and detoxification, promoting body fluid to quench thirst, lowering blood pressure and strengthening the heart, so as to prolong life (document 2 has specific reports). Its main components are saponins, flavonoids, terpenoids, polysac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
Inventor 舒任庚闵雷张普照蔡永红
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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