Monoamine oxidase diagnostic reagent (kit) and method for determining activity concentration of monoamine oxidase
A technology of monoamine oxidase and galactose oxidase, which is applied in the field of medical inspection and measurement, and can solve the problems of difficult measurement, limited practicality, and inability to apply automatic biochemical analyzer analysis, etc.
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Embodiment 1
[0039] The monoamine oxidase diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0040] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0041] Stabilizer 500mmol / L
[0042] Coenzyme 3mmol / L
[0043] Galactose oxidase 6000U / L
[0044] Galactose dehydrogenase 8000U / L
[0045] Benzylamine 5mmol / L
[0046] Galactose Hexose Dialdose 9mmol / L
[0047] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0048] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested monoamine oxidase sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 1 minute, the detection time is about 2 minutes, and t...
Embodiment 2
[0051] The monoamine oxidase diagnosis / measurement reagent of this embodiment is a double reagent, comprising:
[0052] Reagent 1
[0053] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0054] Stabilizer 50mmol / L
[0055] Coenzyme 3mmol / L
[0056] Benzylamine 5mmol / L
[0057] Galactose Hexose Dialdose 9mmol / L
[0058] Reagent 2
[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0060] Stabilizer 500mmol / L
[0061] Galactose oxidase 6000U / L
[0062] Galactose dehydrogenase 8000U / L
[0063] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0064] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested monoamine oxidase sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction directio...
Embodiment 3
[0067] The monoamine oxidase diagnosis / measurement reagent of the present embodiment is three reagents, comprising:
[0068] Reagent 1
[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0070] Stabilizer 50mmol / L
[0071] Coenzyme 3mmol / L
[0072] Benzylamine 5mmol / L
[0073] Galactose Hexose Dialdose 9mmol / L
[0074] Reagent 2
[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0076] Stabilizer 500mmol / L
[0077] Galactose dehydrogenase 8000U / L
[0078] Reagent 3
[0079] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0080] Stabilizer 500mmol / L
[0081] Galactose oxidase 6000U / L
[0082] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0083] When measuring the activity concentration of monoamine oxidase, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, ini...
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