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Experimental animal rat cerebellar ataxia detection model

A technique for ataxia and experimental animals, applied in diagnostic recording/measurement, medical science, sensors, etc., can solve problems such as behavioral and physiological function impairment

Inactive Publication Date: 2010-09-01
成都军区昆明总医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the standard literature on animal models of rat cerebellar ataxia has not been reported, but referring to the standards of animal models of Parkinson's disease, that is, "useful PD animal models are those that can be measured, have impairment of behavioral and physiological functions, and are accompanied by anatomical and biochemical changes", the characteristics of pathological damage designed by this model are relatively in line with or relatively close to this standard

Method used

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  • Experimental animal rat cerebellar ataxia detection model
  • Experimental animal rat cerebellar ataxia detection model
  • Experimental animal rat cerebellar ataxia detection model

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Validity test of rat cerebellar ataxia detection model

[0018] Local anesthesia with 3% sodium pentobarbital (20mg / kg), the rat prone position, the head is fixed on the KW-II mouse brain stereotactic model. After disinfection, the occipital skin was cut along the sagittal line, the skull was exposed, and the bone surface was cleaned with hydrogen peroxide. Refer to the rat brain anatomical location map and the rat brain three-dimensional location map, take the intersection of the sagittal suture and the herringbone suture as the coordinate 0 point, 4.4mm backward, 2mm left or right side, drill a small hole with a diameter of 1mm, The 5μl microsyringe is fixed on the stereotactic model. The tip of the injection needle has a diameter of 20μ, vertically downwards 3-4mm, and a bolus speed of 0.25μl / min. Slowly inject 1μl of KA (10μg / μl) into the left and right hemispheres and vermis of the rat cerebellum After the symptoms of ataxia have stabilized (mostly 12 hours after inj...

Embodiment 2

[0024] Pathologically confirmed

[0025] Pathology confirmed that the KA injection site was accurate. Most of the neurons at the injection site were edema, necrosis, and loss. Under light microscope, rats in the experimental group showed focal necrosis and dissolution of brain tissue, loss of a large number of neurons, brain tissue edema, granular cell nucleus condensation, nuclear fragmentation, red neuronal pyknosis, cell body shrinkage, deformation and other changes ( Picture 9 ). Under the electron microscope, the granular cells of the cerebellar fastigial nucleus in the experimental group appeared edema and large vacuoles were formed in the cytoplasm; the granular cells shrank, their size decreased, their density increased, and the cell morphology was irregular. Purkinje cell endoplasmic reticulum expands. The chromatin of some neurons is concentrated and edge-collected, showing early and mid-term apoptosis changes. The myelin sheath of the myelinated nerve fiber is sepa...

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Abstract

The invention discloses an experimental animal rat cerebellar ataxia detection model, which is characterized by detecting the changes of induced ataxia after cerebellar injury through an experimental animal rat cerebellar ataxia detector via the establishment of the experimental animal rat cerebellar ataxia detection model. The detector is formed by connecting a camera lucida, a camera obscura, a balance rod, a water pool, an electric stimulus pole piece, an adjustable transformer, a switch, a power supply and wires, wherein a plastic pot is arranged between the camera lucida and the camera obscura; the balance rod is arranged above the water pool and is connected with the camera lucida and the camera obscura; and the electric stimulus pole piece in the camera lucida is successively connected with the switch, the adjustable transformer and the power supply via the wires. The detection model is simple, easy to manufacture and operate, objective and sensitive, and has repeatability, scientificalness, reliability and practicability on orientated, quantitive and qualitative researches on rat ataxia.

Description

Technical field [0001] The invention relates to a scientific, objective, quantitative, localized and qualitative model for detecting ataxia in experimental animals, in particular for the detection of cerebellar ataxia in SD rats. Background technique [0002] Ataxia is the cerebellum, proprioception, and vestibular dysfunction that lead to clumsy and uncoordinated movements. It involves limbs, trunk and throat muscles and can cause posture, gait and language disorders. The cerebellum, spinal cord, vestibule and extrapyramidal system jointly participate in the completion of fine and coordinated movements. The cerebellum plays an important role in performing delicate movements. The cerebellum is located in the posterior fossa, the midline is the vermis, and both sides are the cerebellar hemispheres. The cerebellum is an important movement regulation center. The cerebellum maintains body balance, regulates muscle tension, and coordinates body movements. Ataxia is the main symptom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00A61B19/00A61B90/00
Inventor 刘诗翔
Owner 成都军区昆明总医院
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