Method for producing chondroitin sulfate by reverse precipitation

A technology of chondroitin sulfate and reverse precipitation, applied in the biological field, can solve problems such as dehydration, drying, crushing and other operational difficulties, failure to meet customer requirements, precipitation agglomeration and stickiness, etc., to achieve fine particles, short production process, The effect of stable quality

Active Publication Date: 2010-09-08
江苏麦德森制药有限公司
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Problems solved by technology

[0008] Because the chondroitin sulfate solution is relatively viscous, the traditional method is to add alcohol to the chondroitin sulfate solution to precipitate the chondroitin sulfate crystals during alcohol precipitation. During the dehydration and drying process, the organic solvent contained in the crystal is not easy to volatilize due to its large particle shape and hard and dense particle shape, resulting in high solvent residue (above 2%) in the product, which affects product quality and cannot meet customer requirements
Secondly, due to the viscous sedimentation and agglomeration, operations such as dehydration, drying, and crushing become difficult and waste energy

Method used

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Examples

Experimental program
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Embodiment Construction

[0024] (1) Alkaline extraction and enzymatic hydrolysis: Submerge 100Kg of raw material (cartilage) in water, adjust the pH to 8.5 with sodium hydroxide, raise the temperature to 55-60°C, keep it warm for 5 hours, then add 2Kg of alkaline protease to keep the pH at 8.5, Keep warm at 55-60°C and stir for 7 hours, heat up to boiling for about 15 minutes, filter to obtain 500L of filtrate;

[0025] (2) Alcohol precipitation: the chondroitin sulfate solution obtained in step (1) is down to room temperature, under stirring, add sodium chloride 15Kg to the solution, then add 2.5 times of the solution volume with a weight concentration of 95% alcohol 1250L , stand still for 8 hours, and filter to obtain the chondroitin sulfate precipitate;

[0026] (3) Oxidation: the chondroitin sulfate precipitation that step (2) obtains is added into 200L water and dissolves, and 6Kg sodium chloride is added in water simultaneously, makes it contain sodium chloride weight concentration to be 3%, an...

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Abstract

The invention discloses a method for producing chondroitin sulfate by reverse precipitation, comprising the following steps of: adjusting pH of the chondroitin sulfate solution obtained by oxidation to 5-6, controlling the temperature at 15-20 DEG C and slowly adding the solution in 95% alcohol which is being stirred, wherein the weight concentration of alcohol in the whole process is always maintained in 75% or above. The precipitated chondroitin sulfate is loose in texture, easy to dehydrate and dry and not necessary to be smashed and has the advantages of saving energy and production cost.The product is coloury and stable in quality. The content of chondroitin sulfate is above 98%. The product has extremely low content of organic solvent which is lower than 0.2%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an improved production process of chondroitin sulfate. Background technique [0002] Chondroitin sulfate, English name chondroitin sulfate, is a polymeric acidic mucopolysaccharide made from the cartilage of animals (pigs, cattle, horses, sheep, chickens, sharks, etc.), and its molecular structure is β-D-glucuronic acid It is a polysaccharide chain composed of repeating disaccharide units formed by β1→3 glycosidic bonds with N-acetylgalactosamine. It is a white or slightly yellow powder with strong water absorption, easily soluble in water to form a viscous liquid, insoluble in organic solvents such as alcohol, acetone, ether and chloroform. Its salts are relatively stable to heat. [0003] At present, the usual extraction method of chondroitin sulfate is the alkali-enzymatic extraction method, which mainly includes the following steps: [0004] 1) Alkaline extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/08
Inventor 刘榜惠张志斌袁红英
Owner 江苏麦德森制药有限公司
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