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Miniaturized total analysis device formed by six-channel microfluidic chip and quartz crystal microbalance

A technology of microfluidic chips and quartz crystals, which is applied in the direction of instruments, photoplate-making processes on patterned surfaces, and material inspection products, etc., can solve the problems of no sample processing function, limited instrument practical functions, low analysis efficiency, etc., and achieve repair Low cost, low maintenance, same migration time effect

Inactive Publication Date: 2010-09-08
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the detectors used in microfluidic chips are mainly laser-induced fluorescence (LIF) and mass spectrometry (MS). These detection methods still need to further reduce the size of the detection device and reduce the cost. Low analytical efficiency and high cost
[0003] In 2002, Hiroyuki et al. combined microfluidic chip and QCM to realize the detection of non-labeled biomolecules (Anal.Chem.2002, 74, 3592-3598); Thomas et al. used microfluidic chip and QCM to combine Antigen-antibody analysis (Lab Chip, 2008, 8, 1648-1657). Although the above method combines the microfluidic chip and the quartz crystal microbalance to realize the detection of biomolecules, it has no sample processing function, and once Microflow can only be detected by one quartz crystal microbalance (QCM), and the detection of multiple substances in the same sample requires multiple measurements, which limits the practical function of the instrument

Method used

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  • Miniaturized total analysis device formed by six-channel microfluidic chip and quartz crystal microbalance
  • Miniaturized total analysis device formed by six-channel microfluidic chip and quartz crystal microbalance
  • Miniaturized total analysis device formed by six-channel microfluidic chip and quartz crystal microbalance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] See figure 2 with image 3The micro-total analysis device composed of a six-channel microfluidic chip with a pump valve structure and a quartz crystal microbalance is shown, including a six-channel microfluidic chip 9 with a pump valve structure, an upper clip 8 (organic glass material) and Lower clip 10 (organic glass material), quartz crystal microbalance 13, quartz crystal microbalance carrying box 15; between the upper clip 8 and the lower clip there is a six-channel microfluidic chip 9 with a pump valve structure.

[0041] See figure 1 with figure 2 . The six-channel microfluidic chip with a pump-valve structure is made of polydimethylsiloxane with six sample outlets sandwiched (sealed) between the upper substrate 1 (glass material) and the lower substrate 3 (glass material). Siloxane (PDMS) film 2 is made of; six sample outlets 6 are opened on the lower substrate, and gas channels and gas inlet and outlet ports 7 are etched on the upper surface of the lower ...

Embodiment 2

[0056] The micro-total analysis device and related external equipment composed of the six-channel microfluidic chip with a pump valve structure and the quartz crystal microbalance of Example 1 are used.

[0057] Pre-heated 60 ° C volume ratio of 3:1 mass concentration of 98% H 2 SO 4 with a mass concentration of 30% H 2 o 2 The QCM cleaned by the mixed solution was soaked in HS(CH 2 ) 6 In SH and ethanol solution, HS(CH 2 ) 6 SH is adsorbed on the QCM via the Au-SH bond, washed twice with water, N 2 Blow dry (provided by an air tube connected to the air intake device above the edge of the QCM); then modify the HS (CH 2 ) 6 The SH QCM was immersed in a solution containing gold nanoparticles with a diameter of 12 nm, which were adsorbed on HS (CH 2 ) 6 SH on, secondary water flushing, N 2 Blow dry; then fix the processed QCM in the quartz crystal microbalance carrying box, record the oscillation frequency of the six QCM at this time, record it as oscillation frequency...

Embodiment 3

[0062]Using the six-channel microfluidic chip with a pump valve structure in Example 1 and the micro-total analysis device and related exogenous equipment composed of a quartz crystal microbalance, and after hybridization of DNA Oligo-1 and DNA Oligo-2 in Example 2 QCM with an adsorption time of 90 min as a probe.

[0063] Fix the QCM with DNA Oligo-1 and DNA Oligo-2 probes whose adsorption time is 90min in the quartz crystal microbalance carrying box, record the oscillation frequency of the six QCMs at this time, record it as oscillation frequency 3, and make its center coincide with The sample tubes of the six-channel microfluidic chip with a pump valve structure are precisely connected, and the air inlet and outlet channels 11 of the six-channel microfluidic chip 9 with a pump valve structure are connected to the vacuum pump controlled by the solenoid valve and the external source equipment of the gas storage tank , add a concentration of 2×10 particles with a particle size...

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Abstract

The invention relates to a miniaturized total analysis device formed by a six-channel microfluidic chip and a quartz crystal microbalance. The device comprises the six-channel microfluidic chip with a pump valve structure, an upper clip, a lower clip, the quartz crystal microbalance and a bearing box of the quartz crystal microbalance, wherein the microfluidic chip is positioned between the upperclip and the lower chip, and is communicated with an outside source device through the design of clips; the six-channel microfluidic chip with a pump valve structure is in a sandwich structure, and the lower substrate is provided with an air passage and six sample outlets; the upper substrate is provided with the sample inlets and six branches of liquid passages which are in annular symmetrical distribution around the sample inlets and are respectively provided with three micro valves; the other end of each branch of the liquid passage is respectively communicated with the six sample outlets on the lower substrate through six sample outlets on a polydimethylsiloxane film; and sample outlet tubes of the six sample outlet of the lower substrate respectively correspond to the six quartz crystal microbalances. The miniaturized total analysis device can process and detect the sample and obtain multiple groups of data at the same time.

Description

technical field [0001] The invention relates to a micro-total analysis device using a six-channel microfluidic chip with a pump valve structure for sample processing and a quartz crystal microbalance as a transducer, in particular to a six-channel microfluidic chip with a pump valve structure and A micro total analysis device composed of a quartz crystal microbalance. Background technique [0002] Quartz crystal microbalance (QCM) is a sensor that detects trace substances based on the change of electrical signal caused by the quality of the substance. It has the advantages of high detection sensitivity, easy to adjust selectivity, low cost, especially timely detection without sample marking, etc. . It is a broad-spectrum detector, which can be used to test the immobilization of biological sample probes, target recognition and hybridization between probes and targets, and can also be used for protein recognition. Although the QCM sensor has many advantages, it is necessary ...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N27/26G03F7/00
Inventor 李兴长李少华韩建华张建平江龙张雅坤
Owner INST OF CHEM CHINESE ACAD OF SCI
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