Staphylococcal enterotoxin C3 preparation, formulation, dosage and application thereof

A technology of Staphylococcus enterica and Staphylococcus, applied in the field of bioengineering, can solve the problems of no literature publication, changes in antigenic determinants and product activity, etc., and achieves the effects of excellent growth, stimulation of T lymphocyte responses, and improved side effects

Inactive Publication Date: 2010-09-15
时贞平
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] At present, some people have used genetic engineering to prepare Staphylococcus aureus enterotoxin superantigen. For example, Xu Mingkai et al. cloned the SEC2 gene into pET~28a for expression, but the expressed recombinant product had 36 more amino acids than the natural protein, so the antigenic determinant and product activity are more likely to change
[0018] In addition, in the current research on SEC, the main research is SEC1, SEC2, and there is no literature published on SEC3. There is only one CN 1283264C patent, which discloses the invention of SE oral preparations prepared by Staphylococcus aureus, and the preparation of SEC3 is the subject of this patent. A small part, but only defined as isolated from one of the strains of Staphylococcus aureus in CGMCC0341, CGMCC0342, CGMCC0485, CGMCC0165, CGMCC0381, CGMCC0780, CGMCC0662

Method used

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  • Staphylococcal enterotoxin C3 preparation, formulation, dosage and application thereof
  • Staphylococcal enterotoxin C3 preparation, formulation, dosage and application thereof
  • Staphylococcal enterotoxin C3 preparation, formulation, dosage and application thereof

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Embodiment Construction

[0055] 1. Preparation

[0056] The present invention will be further described below by implementing examples, and these descriptions are not to further limit content of the present invention, nor the unique route of the present invention, can be conventional, known, also can be the method that the present invention uses for the first time . Relevant persons should recognize that equivalent replacements or corresponding improvements to the technical features of the present invention still belong to the protection scope of the present invention.

[0057] Culture medium preparation

[0058] 1. Culture medium 1: take fresh pig heart, remove fascia and other accompanying tissues, wash, mince pig heart, add 0.5-3.0 times clean water after mincing, and warm at 60℃-100℃ for 0.5-3.0 hours , filter to obtain the filtrate, add 0.1-20% peptone by total weight, adjust the pH to 2.0-7.0, let it stand at low temperature, then add 0.1-1.0% activated carbon, boil, filter, adjust the pH of t...

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PUM

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Abstract

The invention comprises staphylococcal enterotoxin C3 preparation, formulation, dosage and application thereof, belonging to the field of biological engineering. Staphylococcus aureus is cultivated, separated and purified to prepare the staphylococcal enterotoxin C3 (Staphylococcal Enterotoxin C3, SEC3); SEC3 is prepared into injection and oral formulations, such as oral liquid, capsules, tablets, pellets and the like, or SEC 3 is prepared into fusion protein and then is prepared into injection formulation; the effective dose range of clinic amount of generated formulation performing drug administration through muscle and blood is more than 3ng/day; the effective dose range of clinic amount of generated formulation performing oral administration is more than 800 ng/ day. The SEC3 of the invention is suitable for treating diseases of tumor, virus and immunodeficiency.

Description

technical field [0001] The invention belongs to bioengineering, and relates to the preparation method, purification method, preparation, dosage and application of Staphylococcal Enterotoxin C3 (Staphylococcal Enterotoxin C3, SEC3) in the preparation of tumors, viruses and drugs for regulating body immunity. The preparation, dosage and application fields of SEC3 also include the range of SEC3 obtained by other methods not in the present invention. Background technique [0002] Superantigen (SAg) is a new immunological term first introduced by the Swedish scientist White in 1989. It is a protein molecule encoded by bacteria or viruses. It does not require antigen (APC) processing and is directly bound to the APC membrane in the form of a complete protein molecule. The combination of MHC-II molecules outside the antigen-binding groove leads to the activation and proliferation of T cells with specific Vβ fragments. The number of activated T cells is thousands of times or even te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02A61K39/085A61P31/12A61P35/00A61P37/02C12R1/445
Inventor 不公告发明人
Owner 时贞平
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