Application of icaritin in preparation of anti-angiogenic medicaments

An anti-angiogenesis and icariin technology, which can be used in drug combinations, anti-tumor drugs, and pharmaceutical formulations, and can solve problems such as unreported uses

Inactive Publication Date: 2010-09-22
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preliminary studies of the present invention have shown that icariin is a natural anti-inflammatory drug, and there is no report about the use of icariin in anti-angiogenesis

Method used

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  • Application of icaritin in preparation of anti-angiogenic medicaments
  • Application of icaritin in preparation of anti-angiogenic medicaments
  • Application of icaritin in preparation of anti-angiogenic medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Study on the Impact of Icaritin on HUVEC Migration Experiment

[0023] 1. Cell line

[0024] Human umbilical vein endothelial cell line (HUVEC) was purchased from Shanghai Cell Bank, Chinese Academy of Sciences. DMEM medium containing 10% fetal bovine serum was incubated at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, replace the medium for 48 hours, when the cell growth reaches saturation, digest with 0.25% trypsin + 0.02% EDTA for passage, once every 2-3 days, the experiment uses logarithmic growth phase cells.

[0025] 2. Experimental drugs

[0026] Icaritin and ginsenoside Rb1 were dissolved in DMSO and diluted to corresponding concentrations with DMEM medium.

[0027] 3. Detection of HUVEC cell proliferation

[0028] Adjust the concentration of HUVEC cell suspension to 1×10 5 / mL, added to 96-well culture plate, 100 μL per well, placed at 37°C 5% CO 2 Incubator culture 6h. Aspirate the old cult...

Embodiment 2

[0039] Example 2 Experiment of Icaritin Anti-Angiogenesis of Chicken Embryo Chorioallantoic Membrane (CAM)

[0040] Materials and Methods

[0041] 1. Experimental drug:

[0042] Same as Example 1

[0043] 2. Breeding eggs:

[0044] Select eggs with clean surface, homogeneous shell, 50g-65g weight, standard shape, uniform air chamber and pores, and fertilization rate >90% as the test objects. Wash twice with warm water (40-50°C) before incubation, soak with 1:1000 bromogeramine solution and wipe dry. Place the eggs in an incubator (37.0±0.5°C) to incubate, with the blunt end facing upwards, and place them on a 6-well culture plate at an angle of 45°C. A wet tray is built into the incubator to keep the relative humidity at 65-70%. Turn the eggs twice a day during incubation to prevent the embryos from sticking and to promote the movement of the amnion. Use the transmission light to check the embryonic development every day, identify the signs of embryonic development, and e...

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Abstract

The invention belongs to the field of traditional Chinese medicaments and relates to new medicinal application of a Chinese medicinal active ingredient icaritin, in particular to the application of the icaritin in preparation of anti-angiogenic medicaments. In the invention, the influence of the icaritin on cell proliferation and migration of human umbilical vein endothelial cells is observed through vitro experiments, the influence of the icaritin on the angiogenesis of a chick chorioallantoic membrane is observed through vivo experiments, and results demonstrate that the icaritin can effectively resist the angiogenesis and the anti-angiogenesis is not realized by cytotoxicity. The icaritin can be used as a medicinal active ingredient to be prepared, together with a pharmaceutically acceptable carrier, into anti-angiogenic medicinal compositions.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medical application of icariin, an effective component of traditional Chinese medicine, and in particular to the application of icariin in preparing anti-angiogenesis drugs. Background technique [0002] Angiogenesis refers to the complex biological process in which vascular endothelial cells differentiate and migrate from the existing vascular system to form new microvessels. Studies have shown that there are a variety of growth factors, extracellular matrix components, proteolytic enzymes, and cell adhesion molecules. participated in the process. After Folkman first proposed that tumor growth and metastasis are vascular-dependent in 1971, the regulatory mechanism of tumor angiogenesis and the significance of anti-angiogenesis in tumor therapy have received widespread attention. Studies have shown that when the tumor grows to 1-2 mm, new blood vessels must be pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00A61P43/00
Inventor 董竞成吴金峰吕祥杜娟徐长青刘宝君曹玉雪
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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