Ganoderma lucidum polysaccharide liquid fermentation preparation method using rice wine vinasse as raw material

A technology of Ganoderma lucidum polysaccharide and liquid fermentation, applied in the field of fermentation engineering, can solve the problems of great influence and high cost, and achieve the effects of increasing value, reducing blood sugar, and preventing environmental pollution

Inactive Publication Date: 2010-09-22
湛江三角威力神酿酒集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificially cultivated Ganoderma lucidum generally takes three months to harvest, and it is greatly affected by the climate, and the cost is also high
There are also many reports on the production of Ganoderma lucidum polysaccharides by liquid fermentation, and its fermentation and extraction processes are relatively mature, but there are no reports on the production of Ganoderma lucidum polysaccharides using rice distiller's grains as a low-cost raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Seed culture: put 100ml of seed medium into a 500ml Erlenmeyer flask, sterilize at 121°C for 15 minutes, cool to room temperature, and then insert the activated bacteria that grow on the slope into the seed medium. The seed medium is (unit is g / L): glucose 20, yeast powder 2, KH 2 PO 4 2. MgSO 4 1. The initial pH is the natural pH; the culture conditions are: the shaker rotates at 150 revolutions per minute, and cultures are carried out at 28°C for 6 days.

[0013] The microorganisms used to produce Ganoderma lucidum polysaccharides are strains of the genus Ganoderma, such as Ganoderma lucidum, Ganoderma lucidum and other ganoderma strains. In this example, the strain preserved by the China Microorganism Collection and Management Center with the number CGMCC No. 5.26 was used.

[0014] (2) Liquid fermentation culture: put 100ml of liquid fermentation medium into a 500ml Erlenmeyer flask, sterilize at 121°C for 15 minutes, cool to room temperature, then insert t...

Embodiment 2

[0017] Other conditions are the same as in Example 1, the liquid fermentation medium in the change step (2) is (in g / L): glucose 20, rice distiller's grains 500, soybean meal powder 2, KH 2 PO 4 1. MgSO 4 0.5, Vitamin B1 0.1 The initial pH is the natural pH. Finally, 0.32 g of ganoderma polysaccharide was obtained.

Embodiment 3

[0019] Other conditions are the same as in Example 1, the liquid fermentation medium in the change step (2) is (in g / L): sucrose 18, rice distiller's grains 500, KH 2 PO 4 1. MgSO 4 0.5, the initial pH is the natural pH. Finally, 0.20 g of ganoderma polysaccharide was obtained.

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PUM

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Abstract

The invention discloses a Ganoderma lucidum polysaccharide liquid fermentation preparation method using rice wine vinasse as a raw material. In the method, rice wine vinasse serving as a low cost raw material is fully used to produce the Ganoderma lucidum polysaccharide by sterilization, adding a carbon source and nutrients such as vitamins, inoculating a strain and performing liquid fermentation; and the polysaccharide is extracted by an alcohol precipitation method. The method of the invention can change wastes into valuable materials, prevent the rice wine vinasse from polluting environments, produce the Ganoderma lucidum polysaccharide and greatly improve the value of the rice wine vinasse.

Description

technical field [0001] The invention relates to a method for preparing ganoderma lucidum polysaccharide by liquid-state fermentation using rice distiller's grains as a raw material, and belongs to the technical field of fermentation engineering. Background technique [0002] The production of rice wine in my country is large, and about 0.1t of wet distiller's grains can be produced for every 1t of rice wine produced. Because rice distiller's grains are rich in protein and low in price, they are currently mainly used in the production of feed. However, the production of feed consumes a lot of energy and costs. Therefore, many manufacturers treat rice distiller's grains as waste, which not only causes waste but also pollutes the environment. The solid content of rice distiller's grains is 5-6%. The dry basis of rice distiller's grains contains 47.7% protein, 8.7% fat, and 25% fiber. It also contains rich mineral elements and can be used as a liquid fermentation medium for ga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 叶志中叶盛权余以刚
Owner 湛江三角威力神酿酒集团有限公司
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