Control method for producing 1,3-propanediol by substrate fed-batch fermentation on basis of growth and metabolism characteristics

Abstract

The invention discloses a control method for producing 1,3-propanediol by substrate fed-batch fermentation on the basis of growth and metabolism characteristics, belonging to the technical field of biochemical engineering. The method comprises the following steps of: firstly deriving substrate fed-batch control functions coupled to growth and metabolism characteristics of thallus: logarithm growth period of F1=k1C+k2F2 (1) and stabilization period of F1=b+k3F2 (2); and then fermenting through manual control to determine values of k1, k2, k3 and b, wherein the selection of the fed-batch schemeis based on the concentration of CO2 and comprises the steps of: executing a scheme (1) when Ct+1 > Ct; and executing a scheme (2) when Ct+1 < Ct; and finally building a set of on-line detection and automatic control fermentation system so that the concentration of the substrate in the fermentation process is always 18+ / -2g / L, and the concentration of 2,3-PD is up to 82.64g / L, improved by 33.14 percent in comparison with 55.25g / L by manual control. The method realizes on-line automatic control of the concentration of the substrate in the fermentation process, solves the instability of manual control and effectively improves the concentration of 1,3-PD.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a control technology for fermentative production of 1,3-propanediol, in particular to a substrate coupled with cell growth and metabolic characteristics in the process of batch fed-batch fermentation to produce 1,3-propanediol Logistics plus automatic control method. Background technique [0002] 1,3-Propanediol (English name: 1,3-Propanediol, 1,3-PD for short) is an extremely important chemical raw material with a wide range of applications. 1,3-PD can be used as a synthetic raw material for plasticizers, detergents, preservatives and emulsifiers, and can also be used as an additive in products such as printer inks, detergents, stabilizers and fuel cell fuels to improve product performance; 1,3-PD can be used as an intermediate in medicine and organic synthesis, used in food, cosmetics and pharmaceutical industries; 1,3-PD can replace intermediates such as ethylene g...

AI Technical Summary

Problems solved by technology

However, since the concentration of glycerol is difficult to detect quickly online, the automatic control of the substrate flow during the fermentation process has always been a key problem that is difficult to solve.

Although there are scholars at home and abroad trying to solve this problem, such as German scholars A.Reimann and H.Biebl first proposed the strategy of coupling glycerin feeding and pH adjustment (Biotech Lett, 1996, 18(7): 827-832), People such as Cheng Keke of Tsinghua University adopt the control strategy of combining glycerin and alkali coupling and constant flow adding glycerin (Food and Fermentation Industry, 2004, 30 (4)), the feedback feeding control technology that Tan Tianwei of Beijing University of Chemical Technology etc. proposes (open No. CN101153292A), but these methods are all based on experience to control the glycerol concentration, and do not carry out accurate automatic control according to the characteristics of cell growth and metabolism

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